Posted by admin on Feb 28, 2021 in |
Tuberculosis (TB) is one of the leading infectious diseases and health burdens in the world. One-third of the world’s population, including 40% from India, is estimated to be infected with tuberculosis. Current studies have indicated the urgent need for the development of new, safe, and efficacious drugs to help reduce the global burden of tuberculosis. Novel antimycobacterial scaffolds from natural products have recently been reported. Natural products of plant biodiversity have received considerable attention as potential anti-TB agents since they are a proven template for the development of new molecules against tuberculosis. We have selected eleven medicinal plants on the basis of four criteria to choose a plant for antituberculosis activity. The plants were having antituberculosis effect, hepatoprotective effect, immune-modulatory action, and having the ability to enhance bioavailability. All selected eleven plants were reviewed in ancient literature and research article-based review. So here, our objective is to study pharmacognostic, physochemical and cytotoxicity screening of eleven selected medicinal plants. There are three different extracts of eleven selected medicinal plants that...
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Posted by admin on Feb 28, 2021 in |
Drynaria quercifolia rhizome (DQR) ethnomedicinal information shows the traditional usage of this rhizome for the treatment of body and joint pain, diarrhoea, typhoid, cholera, and skin diseases. The fresh juice of rhizome is still being consumed as an energy drink by tribes and localities of Tamil Nadu and Kerala. However, no investigations have been carried out to claim the anti-arthritic activity of DQR to date. In-vitro studies on anti-arthritic effect of the crude extract were performed. In-vitro anti-arthritic activities were evaluated by using elastase, PLA2, hyaluronidase, and proteinase and assays like protein denaturation inhibition, membrane lysis, and NO scavenging activity. Based on the promising in-vitro results, the use of DQR as an anti-arthritic agent may be agreed upon, and further establishment towards the safety and efficacy is conceded. Acute toxicity study revealed NOAEL (No Observed Adverse Effect Level) up to 2000 mg/kg body weight. Oral administration of 1000 mg/kg body weight (crude extract) for 14 days led to the well tolerance level in albino...
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Posted by admin on Feb 28, 2021 in |
Objective: Stability of In-vitro Diagnostics (IVDs) reagents was performed to check its quality standards, performance, and linearity. IVDs like Calcium reagent containing Arsenazo III were studied by Accelerated stability studies by considering temperature, pH, and light parameters. Materials and Methods: Stability data were obtained by using different instruments like UV spectrophotometer, Biochemistry analyzer, pH meter, HPLC, and HPTLC. This gives information about the degradation that occurred during storage, transportation, etc. Results: Calcium reagent containing Arsenazo III degrade 90.81% when placed at 42 °C by UV spectrophotometer analysis. The stability conditions’ effect on actual serum concentration was measured by taking reagent performance on a biochemistry analyzer. The linearity of reagents decreases at 42 °C and at normal temperature, linearity does not change. HPLC spectra gave degradation of reagent, which was analyzed by its retention time, peak height, and % area. Arsenazo III produces 91.25% remains undecomposed in 3 months when exposed to light. The shelf life of the calcium reagent was found to be 85.36. HPTLC spectra gave degradation of...
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Posted by admin on Feb 28, 2021 in |
Myrica esculenta Buch – Ham. ex D. Don has been used worldwide for the treatment of various ailments. The present work is an attempt to relate the presence of phytochemicals in leaves of M. esculenta with its antibacterial potential using seven different solvents for preparation of extracts. The preliminary phytochemical screening confirmed the presence of alkaloids, flavonoids, carbohydrates, tannins, vitamin C, fats and oils, terpenoids, and phenols. The total phenolic and flavonoid content in the examined plant extract was found to be 97.66 ± 0.16 mg GA/g and 51.3 ± 0.32 mg RE/g of leaf extract, respectively. The maximum zone of inhibition was recorded by aqueous extract at 22.66 mm against Clostridium perfringens followed by 15.66 mm against Shigella boydii. On comparing the antibacterial effect of various extracts, the methanolic and ethanolic extracts are not significantly different from each other in terms of inhibitory effect against the microbes under study. All the bacterial strains were found to be sensitive to at least two extracts of the plant, but the...
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Posted by admin on Feb 28, 2021 in |
Combretum mucronatum Schumach & Thonn is a scandent shrub whose leaves are widely used by Ghanaians and other West-African herbalists for the treatment of various infectious and non-infectious diseases. It is used to treat wounds, coughs, dysentery, worm infestation, neurological disorders and bacterial infections. Some coughs are as a result of mycobacterial infection. Despite the success gained in minimizing antibiotic resistance over the past decades, there still remains a need for new antibiotics. Due to the constant development of multi-drug-resistant bacteria to existing antibiotics, particularly those directed against multidrug-resistant Gram-negative bacteria such as the tuberculous bacteria, new antibiotics are needed. Folklorically used antimicrobial herbs are good sources of new antibiotics. In that regard, the aim of this study was to assess C. mucronatum leaves for its possible mycobactericidal activity and investigate its extracts phytochemically. The macroscopic, microscopic, and physicochemical characteristics were determined as preliminary means of quality assessment of the plant materials. Phytochemically, C. mucronatum is rich in a wide variety of tannins and flavonoids, and their corresponding TLC...
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