Posted by admin on Feb 28, 2021 in |
The effect of constant lighting on the level of some hematological parameters and their daily dynamics was studied in male Wistar rats at the age of 6 months. It was found that staying in constant light for 3 weeks leads to a rise in hematocrit level, mainly due to an increase in the number of red blood cells, while a decrease in the oxygen-transporting properties of these cells is noted. An analysis of the diurnal dynamics of the studied parameters, it was found that the keeping of animals under constant illumination destroys the circadian rhythms of HGB, MCV, RDWc, PCT, MPV, PDWc, and MCHC. The destruction of the circadian rhythms found in control is most likely due to both a decrease in the level of melatonin in constant light and a violation of its own circadian rhythm in the absence of a pacemaker, which role is played by the alternation of light and...
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Posted by admin on Jan 31, 2021 in |
The present UV Spectrophotometric and RP-HPLC method was simple, accurate, precise, specific, and sensitive. Spectrophotometric method was developed in water and very useful for analysis of bulk drug and injection formulation. RP-HPLC method was developed in Phosphate buffer (pH 3.0 adjusted with orthophosporic acid) and acetonitrile (70:30) using column HiQsil C18 column (250 × 4.6 mm; 5 μm). Flow Rate: 1.0 ml / min. The method was validated for the determination of granisetron hydrochloride in bulk and parenteral dosage form. The standard solution of granisetron hydrochloride in water showed maximum absorption at 301 nm with correlation, slope, and intercept 0.9998, 0.04198, and 0.01255, respectively and the percentage recovery of the formulation was 99.965% by UV spectrophotometry. By RP-HPLC, the % RSD of intraday and interday precision was 0.82% and 1.40 %, respectively. The % means recovery amount of Granisetron hydrochloride in 80 %, 100%, 120% was 94.3%, 94.9%, 95.2%, respectively, which reflect that the method was free from the interference of the impurities and other additives during the estimation...
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Posted by admin on Jan 31, 2021 in |
The present study was designed to evaluate the anticancer potential as well as cytotoxity of Chloroform extract of Thulasivettilacv of Piper betle L. is an evergreen perennial creeper belonging to family Piperaceae, and known to possess numerous medicinal properties. Current study focuses on evaluating antiproliferative potentialities of Betel leaves. The antiproliferative effect was tested against two cancer cell lines HeLa and HT 29, while cytotoxity was tested against in a normal myoblast cell line, L6 by MTT assay. Five different concentration of the chloroform extract were used for this study. Qualitative phytochemical analysis of leaf extracts showed the presence of tannins, alkaloids, phenols, flavonoids and glycosides. HeLa cell lines exhibited less antiproliferative activity 25.8 µg/ml ± 0.05% viability at 100µg/ml of extract by MTT assay and the IC50 value was found to be 45.19 µg/ml. In case of HT 29 colon cancer cell line, chloroform extract of plant showed 30.32 ± 0.04% µg/ml viability compared with the L6 normal cell line was24.00 ± 0.08% µg/ml. The IC50 values were...
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Posted by admin on Jan 31, 2021 in |
Fatty acid vesicles are novel vesicles incorporating fatty acids which enhance the brain targeting efficiency when compared to conventional liposomes. This study’s objective was to prepare, characterize, and evaluate fatty acid vesicles for brain targeting of a water-soluble drug, atomoxetine HCl through nasal route. The atomoxetine HCl loaded fatty acid vesicles were prepared by using the reverse phase evaporation method using Phospholipon 90 H, oleic acid, and ethanol. Prepared formulations were evaluated for parameters like particle size (152.9 nm), drug entrapment (90.4%), zeta potential (-9.41 mv), and in-vitro release was found to be 98% up to 4 h. SEM results showed spherical shaped vesicles. The ability of the prepared atomoxetine HCl loaded fatty acid vesicles to deliver atomoxetine HCl through the nasal route was studied by ex-vivo studies using goat nasal mucosa, which showed sustained release of 51% of the drug up to 4...
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Posted by admin on Jan 31, 2021 in |
The removal of metals by the use of microbes is known as bioleaching in terms of oxidation or degradation of metal from complex raw materials or depots. Such microbial oxidation or bio-removal occurs in terms of sulphur degradation and oxidation (for example, sulphur oxidation, iron oxidation etc.). This process is thus known as bioleaching, which is related to the all metal oxidation processes like sulphur oxidation or iron oxidation. These microbes are mild, moderately thermophilic, iron-mineral, sulphur oxidizing bacteria, and extremely thermophilic. The iron bacterium occurs in nature in different genera viz. Thiobacillus ferroxidans, Leptospirillum ferroxidans, Ferrovum myxofaciens and Acidiphilium or Acidithiobacillus. These microbes are the source of enzymes and metabolites which are of industrial significance. The commercial exploitation of such features of iron bacterial consortia may be utilized in water treatment for the removal of iron. The use of such microbes via fermentation technology may be fruitful in the removal of iron from water. Few studies have been done in this aspect. The hypothesis of this concept, although...
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