Posted by admin on Mar 31, 2021 in |
Three simple and economical UV-spectrophotometric methods have been developed and validated for simultaneous estimation of ciprofloxacin (CIP) and metronidazole (MET) in a tablet dosage form using distilled water as a green solvent. The proposed methods were; simultaneous equation method (method A), Q-absorbance ratio method (method B), and area under curve method (method C). λmax of CIP & MET in distilled water were found to be 271 nm and 320 nm, respectively. The isoabsorptive point was observed at 290 nm. The linearity was obtained in the concentration range of 1-9 μg/ml, and 2-18 μg/ml for CIP and MET respectively by methods A, B & C. Validation parameters were carried out. All three methods were found to be linear, accurate, precise, and specific. Good results were achieved using distilled water as solvent due to its greater solubility, reproducible readings with maximum absorbance. Among the three methods, method C was found to be the most sensitive. Hence, this method can be recommended for the routine analysis of this drug...
Read More
Posted by admin on Mar 31, 2021 in |
Hypertension is now defined on the basis of systolic (SBP) and diastolic (DBP) blood pressure levels and classified into stages on the basis of the degree of elevation. The generally recognized cut point for hypertension is an average office BP of 140/90 mmHg or greater, which has been obtained by a recommended standard technique with an accurate manometer and has been confirmed on at least one other occasion. Hypertension is the main cause of high blood pressure (BP), and BP is a major public health issue in India and is rampant among both urban and rural populations. It is the key modifiable risk factor for many diseases. Prescription-event monitoring or PEM involves a methodology in which the safety of new medicines are studied that are used by general practitioners (GPs). PEM is an observational cohort form of pharmacovigilance that is non-interventional, in the sense that nothing happens to interfere with the doctor’s decision regarding which drug to prescribe for each individual patient. In this study, we focused on the...
Read More
Posted by admin on Mar 31, 2021 in |
Nanoemulsionis a special case of emulsion. It is nano-sized emulsion used as a carrier. It has the ability to deliver the drug to the target site. It is a good vehicle for the controlled delivery of the drug. It is prepared by the high energy and low energy emulsification method. Emulsifier plays an important role in stabilizing the nanoemulsion. It is anisotropic and thermo-dynamically stable system of oil, water and surfactant-co-surfactant (Smix) in an appropriate proportion. Nano-emulsion has a droplet size in between 20-200 nm. NE has the ability to encapsulate both the lipophilic and hydrophilic drug in o/w and w/o systems, respectively. Hence, improved solubility, as well as enhanced bio-availability of the drug, can be achieved through nanoemulsion. For stabilizing o/w and w/o type NE, both hydrophilic and lipophilic surfactants are used at once. The present review article is highlighted on various aspects of formulation-development, patents, and clinical trials of NE and its wider application to the different...
Read More
Posted by admin on Mar 31, 2021 in |
Marfan syndrome (MFS) is a common dominant inherited disorder that affects connective tissue, which is associated with mutation of the gene FBN1. The protein encoded by this gene contributes to the final structure of microfibrils. Single nucleotide polymorphisms (SNPs) of this gene links to variations in gene expression phenotypically among patients. Therefore SNPs would be the main target for identification and analysis, which may help in further diagnosis of such life-threatening disorder. In this study, various computational methods have been used to analyze the genetic variations and identify non-synonymous or amino acid-changing SNPs (nsSNP). It can quicken to evaluate a considerable outcome of a mutation before literally doing the lab work. In total, 475 high-risk nsSNPs have been identified using the NCBI SNPs database. Among these nsSNPs, residues are assigned to predict deleterious or disease-related nsSNP. The conservation of functional amino acid residues and secondary and tertiary structure predictions were also reported using various tools. Swiss-Pdb Viewer allows changing amino acid side chains, causing an artificial mutation to the...
Read More
Posted by admin on Mar 31, 2021 in |
Leucas aspera is the medicinal plant commonly known as ‘Thumbai’ found all over India. It is being used in traditional medicine to treat cough, cold, diarrhea, inflammatory diseases, and paste of the plant that has been used to treat the wound to enhance the wound healing process. The plant is reported to have anti-inflammatory, analgesic, antidiarrheal, antimicrobial, antioxidant, and insecticidal activities. The current study has been taken up to evaluate the aqueous extract of L. aspera leaves (LaLE) for its proteolytic activity and its role in the blood coagulation cascade. The concentration of protein in LaLE was estimated to be 1 mg/ml. The proteolytic activity was determined with a specific activity of 2.40 U/mg/ml. The protein banding profile was analyzed on SDS-PAGE. LaLE hydrolyzed fibrinogen, fibrin, and collagen (I and IV) in a dose-dependent manner. LaLE increased the clotting time in prothrombin time (PT) and recalcification time (RT) by 3.87- and 5.43-folds, respectively, indicating its potentiality as an anticoagulant protease. LaLE inhibited ADP-induced platelet aggregation by 2.38-folds, suggesting its...
Read More
