Posted by admin on Oct 31, 2022 in |
A simple, novel, precise, and cost-effective reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated to simultaneously estimate ezetimibe and bempedoic acid in the marketed tablet dosage form. The chromatographic separation was carried out on a Prontosil C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of Acetonitrile: water (60:40 v/v). The flow rate was 1.0 mL/min with detection at 225 nm using UV detector. The retention time for bempedoic acid was 4.7 min, and for ezetimibe 5.7 min. Ezetimibe showed a linear response in the concentration range of 20-60 µg/mL. Bempedoic acid showed a linear response in 180-540 µg/mL concentration range. The correlation coefficient (‘r²’ value) for ezetimibe and bempedoic acid was 0.9982 and 0.9998, respectively. The results of analysis have been validated as per different validation parameters. The percentage recoveries obtained for ezetimibe and bempedoic acid range from...
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Posted by admin on Oct 31, 2022 in |
Streblus asper Lour is traditionally used for anti-diarrheal and anthelmintic activity. The present study aims to prove the antidepressant activity in a different extract of leaves of S. asper Lin animal models. The extracts of Sterblus asper L. significantly reduced the immobility time in the forced swimming test (FST), notable at 400 mg/kg when compared with the control group. The immobility time in the tail suspension test (TST) using mice markedly reduced after acute 14 days of treatment with Despiramine Hydrochloride (30 mg/kg). Administration of extracts of Streblus asper L. at dosages of 100, 200, and 400 mg/kg significantly reduced the immobility time. The extracts of Streblus asper L. (100mg/kg) shown that the same locomotion effect as the control group. However, 200 mg/kg and 400 mg/kg doses of Streblus asper L. significantly increase locomotion, rearing, and defecation. The result obtained from the forced swimming test (FST), tail suspension test (TST), and measurement of locomotor activity test (MLAT) indicate that the ethanolic extract of the leaves of S. asper L....
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Posted by admin on Oct 31, 2022 in |
The proposed study aimed to develop and validate a method for the simultaneous determination of emtricitabine, bictegravir, and tenofovir alafenamide in pharmaceutical dosage form using ultra-ultra dosage performance liquid chromatography (UPLC). The drugs were separated using HSS C18 (100 x 1.8mm, 1.7m) column with a mobile phase composition consisting of 0.1% orthophosphoric acid (pH 2.2) and acetonitrile in the ratio 70:30%v/v at a flow rate of 0.3ml/min. The column temperature was maintained at 30°C, and a detection wavelength of 260nm was used. The retention times were found to be 0.62mins for emtricitabine, 0.89 min for bictegravir and 1.75 min for tenofovir alafenamide. The developed method was validated in accordance with the international conference on harmonization (ICH) guidelines and found to be accurate, precise, specific, and robust. The method obeyed Beer’s law in the 50 – 300µg/ml concentration range for emtricitabine, 12.5 – 75µg/ml for bictegravir and 6.25 – 37.5µg/ml for tenofovir alafenamide, with a correlation coefficient of 0.999. The developed method can be used for the routine quantitative analysis...
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Posted by admin on Oct 31, 2022 in |
Epilepsy is a chronic non-communicable disease that affects 50 million people worldwide. AEDs play a vital role in controlling seizures but are not a permanent solution. For a large percentage of Persons with Epilepsy, seizures happen even with the intake of AEDs. Apasmāra is more or less compared to epilepsy. The majority of studies conducted on Apasmāra were śamana therapies and suggested śodhana therapy for better results. A non-randomized controlled trial on the Add-on effect of Selected Ayurvedic Treatment Protocol in Focal Seizures Evolving to Generalized seizures was conducted to assess and compare the selected protocol’s add-on effect, including śodhana and śamana in secondary generalized seizures. In the trial group, 10 diagnosed subjects with secondarily generalized seizures on Anti-Epileptic Drugs were included and given the intervention. In control, group 10 diagnosed subjects with secondarily generalized seizures on Anti-Epileptic Drugs were observed for the same period. The treatment’s effect was assessed using the Epilepsy severity assessment chart. Assessments were done on 0th day, 15th day, 30th day, 45th day, 60th...
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Posted by admin on Oct 31, 2022 in |
The Petasis boron–Mannich process, also referred to as the Petasis reaction, combines boronic acid, an amine, and a carbonyl derivative in a multi component coupling process. Recent progress on petasis reaction is discussed in this review. The various merits of petasis reaction over the other multi-component coupling reactions are explain here. Noncanonical substrates are used to explore the expansion of a variety of petasis reactions, including two–component, three–component, and four–component reactions, processes, and products. In this review, Microwave–assisted reactions are also explored. The optimal conditions are involved in the microwave heating process. The conditions are successfully applied for petasis reaction. Different solvents are used in petasis boron-mannich reactions, such as glycerol and water. Both solvents are suitable for the reaction and give favorable yield. The reactivity along with numerous synthetic applications of the Petasis reaction are given in this review. The natural product synthesis are given by Petasis boron-Mannich reaction in which loline alkaloid and sialic acid synthesis are...
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