Posted by admin on Sep 30, 2022 in |
The present study aimed to study the effect of the drug Xymedon and its conjugate with L-ascorbic acid exhibiting hepatoprotective activity on the apoptosis of rat liver cells against the background of the influence of hepatotoxic agent tetrachloromethane. Though the general effects of Xymedon and its conjugates with various biogenic acids have been studied, the molecular markers affected by the compounds have not yet been established. Experimental methods of investigation included modeling of toxic liver damage by oral administration of carbon tetrachloride to laboratory Wistar rats. To establish the molecular mechanisms of the antiapoptotic effect involved in the hepatoprotective activity of Xymedon and its conjugate with L-ascorbic acid, the level of early apoptosis markers Akt, BAD, BCL-2, p53, Active Caspase-8 Active Caspase-9 in rat liver homogenates was determined by multiplex analysis method using the MagPix system («MerkMillipore», USA). The study showed that Xymedon and its derivative with L-ascorbic acid exhibited an antiapoptotic effect, significantly reducing the number of early apoptosis markers BAD, Active Caspase-9. Also, the derivative with L-ascorbic...
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Posted by admin on Sep 30, 2022 in |
The aim of the present research work was the in-vitro evaluation of the antibacterial potential of polyherbal hand wash formulation against some skin pathogens. Herbal remedies are widely used in healthcare around the World. Herbal medicines have been widely used as effective preventative and treatment options for various ailments. There are a variety of hand washes available in the market many of them have some adverse effects such as itching, dermatitis, irritation etc. An attempt has been made to produce a polyherbal hand wash to prevent these adverse effects of synthetic handwash formulations. For the preparation of polyherbal hand wash the hydroalcoholic leaves extracts of Moringa oleifera (Drumstick tree), Calotropis gigantea (Giant milkweed), Azadirachta indica (Neem) and lemon juice were used. Two hand wash formulations were prepared, and the formulations were also tested for physical attributes such as pH, viscosity, and appearance. The antibacterial activity of formulated herbal hand wash was tested against skin pathogens such as Escherichia coli, Staphylococcus aureus by the streak-plate method. The results obtained were compared with...
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Posted by admin on Sep 30, 2022 in |
The aim of the present In-silico studies is to find out the potential protein target and find out the 3D structure of crustin antimicrobial protein in Portunus trituberculatus. Crustin are small cationic proteins assumed to be antimicrobial effectors against mainly Gram-positive bacteria. The swimming crab Portunus trituber-culatus is a commercially important crab species in East Asia countries. Gonadal development is a physiological process of great significance to reproduction and commercial seed production for P. trituberculatus. The nucleotide and protein sequence of crustins protein from P. trituberculatus is retrieved from the NCBI database in Fasta format. Further, the primary and functional analysis of crustin protein was done through protparam and helix turn helix tools. The retrieved protein sequence was applied to an advanced protein modeling server Cph to deliver the 3D structure. After modeling, the protein’s three Dimensional structure was viewed with Discovery studio software’s help. Our method enables the production of new antimicrobial peptides as potential next-generation...
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Posted by admin on Sep 30, 2022 in |
The current study aimed at investigating the phyto-chemical constituents, in-vitro antioxidant activity and in-vivo acute oral toxicity of Banana inflorescence (blossom) of Musa acuminate. All the experiments were conducted using standard procedures. The results of the phytochemical screening revealed the presence of different types of secondary metabolites like alkaloids, phenols, saponins, flavonoids, steroids, tannins, and glycosides. The acute toxicity study was conducted in Wister albino rats. The Banana inflorescence was administered as a single dose of 2000 mg/kg body weight and observed the behavioral changes and mortality rates. In this study, no visible behavioral changes and mortality observed up to 2000 mg/kg of the Wister albino rats. The antioxidant activity of Banana inflorescence was evaluated by DPPH free radical scavenging assay. Rutin was used as a reference compound. The IC50 value of Banana inflorescence was found to be 46.47372 % and the standard rutin was 27.69024%. We conclude that Banana inflorescence extract did not exhibit any toxic effects on mice. This extract also possesses many phytochemicals and significant antioxidant activity. Therefore, Banana...
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Posted by admin on Sep 30, 2022 in |
Alzheimer’s disease, the most common form of dementia, is higher in elderly women than men. Escape latency time of Group 3 (Donepezil) and Group 6 (AMEE and Estradiol) is less than group 2 (Scopolamine and letrozole). As shown by the results, the decrease in AChE level in Group 3 (Donepezil) and Group 6 (AMEE and Estradiol) indicates restoration of Ach level in the brain. Increased GSH level was seen in Group 3 (Donepezil) and Group 6 (AMEE and Estradiol16 indicating antioxidant property. The architecture of the neurons in the hippocampus histopathological studies was seen when the combined treatment with estrogen and AMEE was given confirming that our given combination has the potential to improve memory in female rats, particularly those who were estrogen-deficient as in our experimental design. Based on our experiments, we can conclude that Aegle marmelos ethanolic extract, along with estradiol, can improve memory and can be used for further studies in this...
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