Posted by admin on Jul 31, 2022 in |
Objectives: To investigate and compare the analgesic effect of fluoxetine (FLU), duloxetine (DUL) and venlafaxine (VEN) in animal models of chronic pain. Methods: Chronic pain was induced in albino rats using spared nerve injury and vincristine. Rats were divided into Control group/ Normal Saline (NS5), DUL group, FLU group, and VEN group. In spared nerve injury, sural branch of sciatic nerve was kept intact while another two branches, common peroneal and tibial nerve ligated and cut. In the vincristine-induced neuropathic pain model, vincristine was administered along with test drugs for 5 days and then 2 days gap and then for 5 days. Mechanical allodynia and hyperalgesia developed in animals were indicative of pain. Pain assessment was done by using von frey filaments by measuring paw withdrawal threshold (PWT) on day 7 in spared nerve injury and on 7 and 14 days of vincristine administration. Results: In spared nerve injury, DUL and FLU showed a significant increase in PWT compared to the control group. Venlafaxine had no analgesic action in...
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Posted by admin on Jul 31, 2022 in |
Molecular Docking is an effective and competent tool for in-silico screening. It is playing an important and ever-increasing role in rational drug design. The main application lies in this structure-based virtual screening is the identification of new active compounds towards a particular target protein. In present investigation, few N1-chloroacetyl-7-substituted- 4-methyl-1, 5-benzodiazepine-2-one and 7-Substituted-4-methyl-1,5-benzodiazepin-2-one are designed and docked at active site of cavity 1# of GABA-A receptor-associated protein (1KJT) to identify their hypothetical binding mode. As a target protein, we used the X-ray crystal structure of mammalian GABA-A receptor-associated protein from the Protein Data Bank. The Molecular Design Suite was used in this study to conduct docking investigations and conformational analyses. The comparative docking experiments of designed compounds with known GABA agonist, Clobazam was carried out. The dock scores calculated for Clobazam was -5.2598. Among the designed compounds, following conformation were found to have lower dock scores as indicated in bracket; N1-chloroacetyl-7-bromo-4-methyl-1,5-benzodiazepine-2-one, Conformer_C4 (-4.7869) and 7-chloro-4-methyl-1,5-benzodiazepin-2-one, Conformer_C13 (-5.0485) and said to have better affinity for active site of GABA-A receptor-associated...
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Posted by admin on Jul 31, 2022 in |
Worldwide antimicrobial resistance is a serious problem and differs from region to region. This study has designed the determination of the incidence and sensitivity pattern of Escherichia coli (E. coli) and discusses the general issues related to antimicrobial resistance. Prospective study was carried out between JAN to April 2020. Samples of urine, blood, pus and miscellaneous (fluids, swabs and sputum) were collected from indoor patients. The present antimicrobial susceptibility of E. coli, the study was performed in the Department of Microbiology Narayana Super-specialty Gurugram, Total of 315 samples were tested out, 97 samples showed growth on culture and out of 97 samples, 40 E. coli have isolated and tested antibiotics sensitivity, it has been demonstrated that different samples showing a significant amount of antimicrobials Sensitivity and resistance to different antibiotics (Table 2). Antimicrobials, namely Meropenem have shown sensitivity (65%), imipenem (60%), Colistin (70%). Thus, there is a need for the implementation of strong antimicrobial stewardship program and stop misuse of antibiotics with an instant effect. Our region’s present study...
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Posted by admin on Jul 31, 2022 in |
Background: Syzygium cumini (SC) usually acknowledged as Jamun. All components of the Jamun may keep used medicinally because of a wide range of diseases like cough, diabetes, dysentery and inflammation. Objectives: Present investigational on the anti-diabetic activity of the methanolic extract seeds of SC in STZ-induced Type I Diabetes in rats. Methods: Extracted SC was to be evaluated the toxicity as OECD 425 guidelines, and biochemical, hematological then gross pathological evaluation has been assessed. Type I diabetes has been triggered in Wistar rats via STZ 65mg/kg/b.w. I.P. During the experiment, Rat’s body weight and blood glucose level were monitored. At the end of the study, animals amongst complete groups were sacrificed, and biochemical parameters; Lipid profile, C-Peptide, HbA1c, Serum insulin, pancreatic insulin, and histology on the pancreas were performed. SC was also screened for pro-inflammatory cytokines viz., IL-1β, IL-6 and TNF-α had been performed via ELISA. Furthermore, Antioxidant Enzymes as SOD, CAT, LPO, and GSH were also performed. Results: The observed extract SC was proven safe among the toxicity...
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Posted by admin on Jul 31, 2022 in |
Iron deficiency anaemia afflicts a large proportion of the human population, compromising productivity and quality of life. Iron supplements are widely prescribed for treating anaemia. However, poor compliance due to side effects, particularly gastrointestinal side effects, remain a hurdle in alleviating the problem, leading to unabated high prevalence. Thus, a fresh look at novel approaches to supplementation is essential. Iron toxicity is attributed to the Fenton Reaction and generation of reactive oxygen species leading to oxidative stress. Several studies suggest that the FR and ROS caused by excess iron, results in chronic inflammation, leading to metabolic syndrome, diabetes mellitus, atherosclerosis, cancer, neurodegenerative disorders. Iron chelates have been developed as treatment modalities to reduce free radical generation, reduce GI side effects, and enhance absorption. Classical Fe chelators, developed to counteract iron overload/ toxicity, cannot be used in IDA, due to severe side effects. In Ayurveda, iron formulations are prepared using phytomolecules that chelate iron and/or reduce OS. These phytomolecules are metabolized by the gut microbiota, which plays an important role...
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