Posted by admin on May 31, 2023 in |
Antibacterial activity of methanolic extracts of different parts (leaves, flowers, green pods and roots) of Moringa oleifera plant were evaluated against gram positive bacteria Bacillus subtilis (Bs), Staphylococcus aureus (Sa) and gram negative bacteria Klebsiella pneumonia (Kp), Escherichia coli (Ec), Pseudomonas aeruginosa (Pa). The activities were performed by the agar well diffusion and cup plate method, with methanol as the negative and tetracycline as a positive control. Secondary metabolites extracted from all the parts of M. oleifera were found active against bacteria. Furthermore, the antimicrobial activity of the mentioned plant extracts was also examined against various gram-positive and gram-negative bacterial strains. It was observed that against K. pneumonia, leaves extracts (17.6 mm) of M. oleifera possess higher antibacterial activity as compared to others, flower extract (19.2mm) against B. subtilis, leaves extracts (22.6 mm) more effective against E. coli, pod extracts (22.5 mm) against S. aureus and leaf extract (21.9 mm) showed against P. aeruginosa as compared to others. The results reveal that M. oleifera might be a potential source...
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Posted by admin on May 31, 2023 in |
Curcumin is a phytochemical isolated from Curcuma longa (Zingiberaceae) and has been used by ancient people for a long time to treat various ailments. Piperine is the main bioactive constituent that has been isolated from Piper nigrum L. Piperine increases the bioavailability of curcumin. Several analytical methods have been developed for the detection of curcumin and Piperine like high-Performance Liquid Chromatography (HPLC), high-performance thin layer chromatography (HPTLC), ultraviolet (UV) spectroscopic methods, Ultra-performance liquid chromatography (UPLC), and hyphenated methods. Numerous analytical techniques have been developed to quantify curcumin and piperine; most focus on accomplishing this separately. Only a few techniques have been created to determine curcumin and piperine in the combined dosage form simultaneously. The main purpose of this review is to describe a brief overview of the method development for Curcumin and Piperine in pharmaceutical dosage form, biological fluids, and Herbal...
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Posted by admin on May 31, 2023 in |
Endophytes constitute a remarkably multifarious group of microorganisms ubiquitous in plants and maintain an imperceptible association with their hosts for at least a part of their life cycle. Their enormous biological diversity, coupled with their capability to biosynthesize bioactive secondary metabolites, has provided the impetus for a number of investigations on endophytes. There is a need to search new ecological niches for the potential of natural bioactive agents for different pharmaceutical, agriculture, and industrial application; these should be renewable, eco-friendly, and easily obtainable natural products discovery in the search for new drugs and is the most potent source for the discovery of novel bioactive compounds. Therefore, a large number of bioactive compounds are isolated from plants, bacteria, fungi, and many other organisms. Endophytic fungi, the most promising of these, have been a source of various such bioactive compounds. Many of these compounds are being used for the treatment of a number of diseases. This review emphasis on the biology of fungal endophytes, their discovery, isolation, identification by morphological and...
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Posted by admin on May 31, 2023 in |
The continuous search to search for a naturally occurring medicinal compound that could be a suitable adjunct to existing therapies for treating one of the growing global health emergencies in diabetes is a never-ending one. Naringenin [2, 3-dihydro-5, 7-dihydroxy- 2 -(4-hydroxyphenyl) -4H-1-benzopyran-4-one], a naturally occurring flavonoid abundantly present in grapes, grapefruit, and citrus fruits have been found to confer different pharmacological activities. Thus, keeping its potential medicinal properties in mind, our aim of the study was an in-depth study of intraperitoneal treatment of naringenin, especially at the levels of oxidants, lipidemic activities, and anti-apoptotic activity in tissues such as the heart and brain, so that it could be better evaluated as a potential therapeutic agent. Our study demonstrates that naringenin treatment reduced hyperglycemia-inducedoxidative stress by activating various intracellular anti-oxidant enzymes and preventing apoptosis by initiating the translocation of Bcl-2 to the mitochondria, partially affecting the release of mitochondrial cytochrome c to the cytoplasm and preventing the activation of caspase-3 and caspase-9, thus preventing cell death. The transmission electron microscopic...
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Posted by admin on May 31, 2023 in |
Leukemia is a disease of cancer that affects blood-forming tissues i.e., bone marrow and lymph nodes, which is associated with uncontrolled growth of WBCs. These cells are unable to fight against infection and weaken the ability of bone marrow to make RBCs and platelets. When leukemic cells overproduce, they interfere in circulatory cells’ functioning, i.e., RBCs and platelets. Along this, leukemia’s pathogenesis is unique in relation to different malignant growths which ordinarily start in major organs and then spread in bone marrow. Different types of leukemia are discovered based on different classification systems with the two major leukemia types – acute and chronic. The work includes the 50-year history of leukemia-lymphoma cell lines, which is considered a key model system in biomedicine. The work involves finding suitable protein receptors for the available potent drugs. Protein receptors are the target sites where drug molecules bind, activate and start their response. FLT3 tyrosine kinase protein is taken as a receptor to study docking concerning ligands like Abacavir, Gabapentin, etc. Autodock and...
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