Posted by admin on Oct 31, 2021 in |
Actino bacterium Streptomyces sp. NLKPB45, which was isolated from mangrove soil sediments of Nellore coastal regions of Andhra Pradesh, India, was used in shake-flask fermentation to explore the different carbon and nitrogen substrates for antimicrobial crude compound. Careful experimentation of ranging each variable in the fermentation medium found that glucose and peptone as preferred carbon and nitrogen sources at 1% (w/v) concentration each, at pH of 7 and 28 °C incubation temperature for 8 days of incubation time. Response surface methodology-based statistical optimization of the process variables with their interconnection was analyzed using a central composite design. A second-order quadratic polynomial equation yielded a complacent fit for experimental with respect to crude compound concentration. Analysis of variance statistics showed that significant-high R2 value for model and adjusted and predicted; R2 values were also shown decent agreement with observed and predicted results. The results have confirmed very good interaction among five process variables at optimized values of 7.51318 pH, 28.734 °C incubation temperature, 8.18409 days incubation time, 1.05185 g/L glucose...
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Posted by admin on Oct 31, 2021 in |
Background: Aloe barbadensis (AB) is a member of the Liliaceae family, traditionally used for thousands of years in some parts of India and well known for its anti-inflammatory, antimicrobial, and wound healing properties. The purpose of the study is to evaluate the antioxidant, anti-angiogenic and antiproliferative effect of AB methanolic root extract on A549 lung cancer cell line. Methods: The Antioxidant activity of AB methanolic root extract (ABMRE) was tested using DPPH. The antiproliferative effect against A549-lung cancer cell lines was tested following MTT assay and their anti-migratory activity was tested by performing wound healing or scratch assay. Antiangiogenic property of root extract was determined by measuring the expression of VEGF protein in lung cancer cell by immunoblot technique and in-vivo CAM assay. Results: The ABMRE inhibited DPPH radical up to 50% at the concentration of 60 ± 0.04 µg/ml having high phenol content of 247.50 ± 21.39 mg GAE/g. The cytotoxic effect of ABMRE against A549 cells and their IC50 values were 7.429 ± 0.559, 5.370 ± 0.693,...
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Posted by admin on Oct 31, 2021 in |
The increasing microorganism resistance to common antimicrobial drugs constitutes a major public health issue worldwide and particularly in Gabon. The objective of this study was to conduct an ethnobotanical survey among merchants of Peyrie market in Libreville (Gabon) to identify plants or a combination of plants used to treat malaria in traditional medicine. Information was collected by interviewing merchants of the market using a structured questionnaire. Plant samples were identified and authenticated at National Herbarium at the Institute of Pharmacopeia and Traditional Medicine in Libreville, Gabon. A literature review of the collected species was done. Twenty-nine (29) plants were identified, and some of them are often used in combination. Decoction, maceration, and herbal teas were the different methods of preparation. Barks, leaves, and fruits were the most plant parts used. Overall, some of these plants have already been investigated for their antimalarial properties, such as Momordica foetida (Cucurbitaceae) and Enantia chlorantha (Annonaceae). The data also highlighted several chemical compounds in the plants which can be responsible for the antiplasmodial activity described,...
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Posted by admin on Sep 30, 2021 in |
Heterocyclic compounds represent the important structural key in pharmaceutical medicinal chemistry. In literature, five-membered heterocycles are reported to be a core moiety of various pharmaceutical drugs. Oxadiazole or furadiazole is a five-membered heterocyclic nucleus and is considered to be derived from furan by replacement of two methane (-CH=) groups by pyridine type nitrogen. Oxadiazole is a versatile lead compound for designing potent bioactive agents. 1, 3, 4-oxadiazole having verities of potential biological activities can be synthesized by various methods. Hence, nowadays, researchers have developed innovative methods for the synthesis of 1, 3, 4-oxadiazole derivatives and their medicinal applications. The activities include anticancer, antimicrobial, anti-inflammatory, anti-HIV, anti-tubercular, anti-diabetic, antifungal, etc. In this review article, we have summarized various methods for synthesis of derivatives of 1, 3, 4 – oxadiazole nucleus and evaluation of various biological activities. The information in the present article may be useful to many researchers, which leads to the exploration of new therapeutic species for...
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Posted by admin on Sep 30, 2021 in |
Cow urine is considered a Sanjeevani–a medicine capable of curing any disease according to ancient manuscripts from Vedic times. The present study was conducted to validate the medicinal properties of cow urine in a scientific manner. The cow urine sample was procured from Indian indigenous cow Bos indicus from Bombay Panjrapole, Bhuleshwar, Mumbai, and commercial gaumutra from the local market. Various treatments were given to the freshly collected cow urine viz. Photoactivated urine (PA), Cow urine distillate (DIS) and Crude cow urine sample (CRU) was used after filtration and centrifugation. In-vitro assays for antimicrobial (agar cup method), antioxidant (DPPH assay), anti-hyperglycemic (α-amylase inhibition) and anti-inflammatory (protein denaturation inhibition bioassay) properties of cow urine were carried out. Results confirmed antimicrobial (19 mm zone of clearance), antioxidant (157 µg /ml), anti-hyperglycemic (2.08 µg /ml) and anti inflammatory (23.72µg /ml) and comparable to Chloramphenicol, Ascorbic acid, Acarbose, and Ibuprofen used as standard respectively. The study demonstrates various medicinal properties of cow urine using in-vitro...
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