Posted by admin on Feb 29, 2020 in |
Periodontitis is a group of inflammatory pathological conditions involving periodontal ligaments and supporting alveolar bone which may result in the degeneration of supporting structures of teeth ultimately leading to tooth loss. Present research work is focused on the formulation, optimization and evaluation of chitosan microspheres and chitosan- hydroxyapatite biocomposite microspheres to have controlled release of herbal drug quercetin, of which anti-microbial, anti-inflammatory and wound healing properties are well proven. Mucoadhesive chitosan would retain the drug-loaded microspheres at the affected site and hydroxyapatite used in its combination would improve mechanical strength, osteoconductivity and sustain the release of the drug. Microspheres were formulated by solvent-free precipitation technique using the alkaline solution (NaOH solution). These microspheres were optimized using 32 factorial design using design expert software. Optimized chitosan microspheres (QC5) and chitosan-hydroxyapatite microspheres (QCH5) were characterized for surface morphology by SEM and were found to be spherical with a particle size of 5.56 µm and 7.22 µm and entrapment efficiency of 80.74 and 82.97% respectively. The entrapment of drug in microspheres was...
Read More
Posted by admin on Feb 29, 2020 in |
The present study was carried out to evaluate phytochemical composition and pharmacological activities of leaf extract of Lusia zeylanica by using different solvents. The solvents like n-hexane, ethyl acetate and methanolic were used to extract dried leaf material of L. zeylanica. These extracts were analyzed for phytochemical constituents with GC-MS analysis and antimicrobial activity tested against four bacterial strains and three fungal strains by using the agar diffusion method. In-vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenols, saponins, tannins, and terpenoids. GC-MS analysis determines the presence of 11 compounds in ethyl acetate, 7 compounds in methanolic extracts and a total of 7 unknown compounds respectively. A significant cancer cell growth inhibition was observed for two extracts with IC50 values ranging between 18.36 µg/ml to 67.914 µg/ml. Our result shows this plant is a promising source of phytocompounds with potential antimicrobial and anticancer...
Read More
Posted by admin on Jan 31, 2020 in |
Bacteria have proved remarkably developing resistant to a known antibiotic; however, Medicinal plants have manifested notably useful for the control of bacterial infections. This study aimed to isolate and identify the multidrug-resistant bacteria from wound infection, then examine the antibacterial activity of different concentration of the ethanolic extracts of Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis plants against it. One hundred specimens collected from wound infection, isolation and identification done according to standard methods. Antibiotics susceptibility test performed using the Kirby-Bauer disc diffusion method for: Cotrimoxazole (25 μg) Ciprofloxacin (5 μg), Gentamicin (10 μg), Imipenem (10 μg), Erythromycin (15 μg), Tetracycline (30 μg) Methacillin (5 μg), Ceftazidime (30 μg). 94 isolates showed bacterial growth, 40 (42.6%) isolates were Gram-positive bacteria (Staphylococcus aureus) and 54 sample Gram-negative bacteria; 15 (27.8%) Pseudomonas aeruginosa, 13 (24.1%) Proteus spp, 12 (22.2%) Escherichia coli, 10 (18.5%) Klebsiella pneumonia, 4 (7.4%) Citrobacter freundii. Ethanolic extracts from Sudanese medicinal plants namely Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis, screened for their antimicrobial properties against isolated multidrug-resistant...
Read More
Posted by admin on Jan 31, 2020 in |
Mupirocin is a topical antibiotic which is very effective against staphylococci, streptococci and Methicillin-Resistant Staphylococcus aureus (MRSA) infection. Because of its high effectiveness against MRSA infection, it is frequently used in clinical practice for decolonization of MRSA. Due to the increased use of mupirocin, increased resistance rates have been reported. Essential oil is a natural antimicrobial agent which kills the bacteria without promoting the acquisition of resistance. Hence, the combination of mupirocin with essential oils would be an ideal solution for preventing mupirocin resistance. Therefore, in this study we have investigated the antibacterial effect of mupirocin in combination with 3 essential oils (cinnamon, eugenol, and eucalyptus). Zone of inhibition test using disk diffusion method and Minimum Inhibitory Concentration (MIC) test using broth dilution method was carried out to assess the antibacterial activity against S. aureus. In this study, we have checked the type of interaction (synergistic, antagonistic, indifference, additive effect). From the results, it was observed that all the essential oil showed potential synergistic antibacterial effect against S. aureus....
Read More
Posted by admin on Jan 31, 2020 in |
Plants are a valuable source of secondary metabolites. Many phytochemicals are obtained from the plant sources that are used as valuable medicinal agents. Chalcones are one of the important secondary metabolites obtained from many edible plants like foods, vegetables, tea, spices and natural foodstuffs. Chalcones considered as obligate intermediated in flavonoid biosynthesis but they do not accumulate to an appreciable degree in most plants. Chalcones are a group of plant-derived polyphenolic compounds belonging to the flavonoids family that possess a wide variety of cytoprotective and modulatory functions, which may have therapeutic potential for multiple diseases. The largest number of natural chalcones has been isolated from species of the Leguminosae, Asteraceae and Moraceae families. Chalcone accumulating plants have often been used in traditional medicine and These chalcones and their derivatives have shown important antimicrobial, antifungal, anti-mycobacterial, antimalarial, antiviral, anti-inflammatory, antioxidant, anti-tumor, antileishmanial, anticancer properties. The purpose of this review is to summarize the various naturally occurring chalcone compounds which have been isolated from different plants and to describe the recent...
Read More
