Posted by admin on Mar 1, 2016 in |
Vesicovaginal fistula (VVF) is an abnormal fistulous tract that extends between the bladder and the vagina – thus allowing the continuous involuntary discharge of urine into the vaginal vault of the affected patient. This study evaluated presumptively the prevalence and antibiogram of pathogenic bacteria isolated from pre- and post-surgery VVF patients. Urine samples were collected in duplicates from the VVF patients; and these were bacteriologically analyzed using cysteine lactose electrolyte deficient (CLED) agar, MacConkey agar and blood agar. Susceptibility studies were carried out on the isolated pathogens using the disk diffusion method. Klebsiella pneumoniae, Escherichia coli, S. pyogenes and S. aureus were the most isolated bacteria from the urine samples of pre- and post-surgery VVF patients. The organisms showed highest levels of resistance to ceftazidime, cotrimoxazole, amoxicillin, nitrofurantoin, cefuroxime, amoxicillin-clavulanic acid and tetracycline. With the exception of some S. aureus isolates, all the organisms including K. pneumoniae, E. coli and S. aureus were completely resistant to imipenem. However, gentamicin, ciprofloxacin, and ofloxacin showed appreciable levels of antimicrobial efficacy against...
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Posted by admin on Jan 31, 2016 in |
Zingiber cernuum belongs to family Zingiberaceae is a perennial herb, also called as Curved stem ginger. The rhizomes of Zingiber cernuum is steam distilled and the composition of essential oil was studied. The essential oil obtained was yellow in colour and the yield was about 0.007%. The chemical profile of the oil was obtained from their GC-MS analysis. The major components present in the oil are trans-Caryophyllene, α-Humulene and δ-3-Carene. The antimicrobial activity of the essential oil was studied using disc diffusion method. The essential oil was tested against four pathogenic stains of gram positive and gram negative bacteria. The essential oil of Zingiber cernuum show considerable antibacterial activity. The oil was found to be active against both gram positive and gram negative bacteria. The anti bacterial activity is mainly due to the major components present in the oil or due to the synergistic effect of the major and minor...
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Posted by admin on Jan 31, 2016 in |
The aim of the present study is to probe the antimicrobial activities of methanol leaf extract of Leucas sp. Antifungal susceptibilities of certain clinically isolated dermatophytes to methanol extracts of Leucas aspera and Leucas zeylanica leaves were performed using agar well diffusion method. The result obtained shows that all the extracts expressed remarkable antifungal activity with zone of inhibition ranging from 5 to 10mm. Maximum inhibition zone was recorded with Penicillium sp. (10 mm) while minimum inhibition zone of was recorded for Candida tropicalis (3 mm). From the study it is evident that Leucas aspera and Leucas zeylanica possess potential antidermatophytic activity and further study on these plants may lead to explore novel bioactive...
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Posted by admin on Jan 31, 2016 in |
Various parts of the plant Butea monosperma have been used traditionally for many of the diseases like anti-inflammatory, antimicrobial, anthelmintic, antidiabetic, diuretic, analgesic, antitumor, anticancer, astringent activities. Among the various parts of the Butea monosperma first choice is selection of leaves in our current research work because only scanty work has been done on leaves. So it is an attempt to carryout phytochemical evaluation of various leaf extracts and to isolate active constituents and study the characterization of isolated compounds by FTIR, NMR and Mass spectroscopy. The isolated components evaluated for the pharmacological activities along with antimicrobial studies. The pharmacological studies of Petroleum ether, hexane, Chloroform, Ethyl acetate and Ethanol extracts of Butea Monosperma leaves shows the anti-inflammatory activity and anthelmintic activity. The petroleum ether and chloroform extract of Butea Monosperma leaves shows antioxidant activity. The Petroleum ether, Hexane, Chloroform, Ethyl acetate and Ethanol extracts of Butea Monosperma leaves have no anti bacterial and antifungal...
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Posted by admin on Jan 1, 2016 in |
To evaluate the antimicrobial activity of Octenidine (OCT) 0.1%, Chlorhexidine (CHX) 0.2% against bacterial strains of Enterococcus faecalis and Staphylococcus aureus. The strains were inoculated in 7ml of brain heart infusion broth and diluted to reach the concentration equivalent (0.5 McFarland standard). 1ml of organism suspension was contacted with 1ml of each mouthwash and was removed at time interval of 3,5 and 10 minute and plated on Brain Heart Infusion agar. After 72 hours of incubation, colony counts were measured using stereomicroscope. Kruskal Wallis test was conducted on mean number of CFU. Post-hoc tests were conducted by using the Mann Whitney U test and Duncan’s-test of multiple comparisons. The results showed that OCT 0.1% was found to be the most effective in substantially reducing total bacterial counts after 3, 5 and 10 min time interval. OCT 0.1% was found to be the most effective in substantially reducing total bacterial...
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