Posted by admin on Nov 1, 2014 in |
The aim of the present study was to design the suitable culture medium and also optimize the culture conditions for enhanced production of antimicrobial metabolites by Streptomyces violaceoruber VLK-4 which exhibited a broad spectrum of activity against bacteria and fungi. Production of bioactive metabolites by the strain was high in modified yeast extract-malt extract dextrose (ISP-2) broth as compared to other media tested. Mannitol (0.4%) and asparagine (1%) were found to be the most suitable carbon and nitrogen sources for the optimum production of bioactive metabolites as well as growth. Maximum production of bioactive metabolites was found in the culture medium with an initial pH 7.0 incubated for five days at 30 ºC under shaking conditions. This is the first report on the optimization of bioactive metabolites by Streptomyces violaceoruber...
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Posted by admin on Nov 1, 2014 in |
The novel Co (II) and Zn (II) complexes have been synthesized by reaction of CoCl2/ZnCl2 with Schiff bases of benzoyl derivative of 3-methyl-1-phenyl-2-pyrazoline-5-one and sulfa drugs in 1:2 ratio. The synthesized complexes were screened/tested for their antimicrobial activity against pathogenic bacterial strains i.e. E. coli, Staphylococcus aureus strain I, Staphylococcus aureus strain II, Staphylococcus aureus strain III, Vibrio cholera, Gram positive Cocci, Gram positive Bacillus, Gram negative Bacillus, Bacillus subtilis and Salmonella typhimurium. It was found that metal complexes have more antimicrobial activity than their parent Schiff bases. Complexes of Schiff base ligand sulfamerazine and bmphp (L2) were also found to be more...
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Posted by admin on Nov 1, 2014 in |
The phytochemical screening of the bark of Albizia lebbeck (L) showed positive test for tannin, alkaloid, flavonoid, terpenoid, phlabotannin, saponin, steroid and cardiac glycoside. In this article an attempt was made for biological studies (the antimicrobial activity, cytotoxicityand free radical-scavenging activity) of different extracts (hexane, methanol, ethyl acetate, water and butanol extract) of the bark of Albizia lebbeck. The antimicrobial sensitivity of the extracts was tested using the disc diffusion method. The cytotoxic effects of five extracts (hexane, methanol, ethyl acetate, water and butanol extract) of the bark of Albizia lebbbeck were carried out. Free radical scavenging activity of the bark of Albizia lebbeck was performed. All the extracts showed moderate anti microbial activity and gram positive bacteria were more sensitive than gram negative bacteria. Significant cytotoxic activity was noticed and hexane extract was found the most lethal to brine shrimp nauplii. This result revealed that the highest cytotoxic constituent may be non polar in nature. Good free radical scavenging activity was observed in all the extracts, and...
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Posted by admin on Nov 1, 2014 in |
Medicinal plants contribute in human health care system. Most of the plants utilized by village peoples as a folk medicine. The effects of plant extracts on bacteria have been studied by a very large number of researchers in different part of the world. In this study antimicrobial activity of Acalypha indica were investicated against two strains of Human pathogenic bacteria. A total of 15 pus samples were collected from Namakkal private hospital, among that two strains were isolated as Staphylococcus aureus and Pseudomonas aeruginosa. Isolation and identification of bacterial isolates by using standared biochemical tests. The above isolated organisms were tested for their sensitivity towards the Acalypha indica medicinal plant leaves extract by disc diffusion and Agar well methods. In this study the highest antibacterial activity were observed in water extract of Acalypha indica than compared with acetone extract. Inhibitory effect of medicinal plant against Staphylococcus aureus in well diffusion is (17mm), and Psedomonas aeruginosa is (20mm) in water extract. In acetone extract 7mm, 9mm inhibitory zone showed by...
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Posted by admin on Oct 1, 2014 in |
Purpose: The focus of the present paper was a preliminary phytochemical and TLC analysis of the various extracts (root, stem, and leaf) of the mangrove plant Excoecaria agallocha L. Methods: Phytochemical analysis was carried out using the standard phytochemical assays. TLC analysis of the petroleum ether and chloroform fraction of the leaves was carried out using the solvent system Toluene: Ethyl acetate (in 9:1 ratio) and Toluene: Ethyl acetate: Formic acid (in 5:5:0.2 ratio) for PE and chloroform fractions respectively. Result and Conclusion: The results of the preliminary phytochemical screening revealed the presence of various chemical compounds like alkaloids, glycosides, flavonoids, carbohydrates, anthraquinone, tannins, phenols, terpenoids, fixed oil, and fats. The organoleptic study revealed the specific nature of the plant parts used in the study. The behavior of the powdered plant parts in the presence of various chemicals was studied. TLC analysis of the petroleum ether and chloroform fraction of the leaves of Excoecaria agallocha L. revealed bands indicating the presence of various compounds that could act as potential...
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