Posted by admin on Apr 1, 2012 in |
Metal nanoparticles have been using as an ingredients in the preparation of complementary medicines to cure different diseases is an age old medicinal practices. The plant based Ayurvedic preparations are preferred by 80% of the world population and WHO is encouraging the green medicine due to its less side effects. Hence an attempt has been made to screen this important medicinal plant for secondary metabolites biogenesis of silver nanoparticles (SNPs) and their antimicrobial efficacy. The stem, roots, stembark and leaves were selected for synthesis of SNPs. The SNPs formation was confirmed with the help of UV-VIS spectroscopy and characterized by AFM. The phytosynthesized SNPs were tested for antibacterial and antifungal activities using paper disc diffusion method. The results indicate that the selected plant revealed that it is source for different secondary metabolites like anthocyanins, emodins, triterpenoids, tannins, saponins, luecoanthocyanins, reducing sugars, flavonoids, lignins, anthroquniones, phenols and glycosides. But steroids, coumarins, fatty acids and alkaloids are absent. The SNPs are having more anti microbial efficacy than that of the plant...
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Posted by admin on Apr 1, 2012 in |
The research work was conducted with the fruits of Emblica officinalis (Fam: Euphorbiaceae) to investigate antibacterial & antifungal activities. The fruits of the plant were successively extracted by cold extraction process by using two solvents namely ethanol and acetone. Antimicrobial activities of the extracts of both solvents were investigated by a simple agar diffusion method using ten pathogenic bacteria. The extracts of ethanol showed moderate activity against Staphylococcus aureus, Salmonella typhi, Bacillus subtilis, Shigella dysenteriae and Bacillus megaterium. Again acetone extracts showed moderate activity against Vibrio cholerae, Staphylococcus aureus, Bacillus subtilis and Shigella dysenteriae.All the activities were determined by measuring the zone of inhibition compared with the standard antibiotic (Amoxicillin). Antifungal screening was done for the ethanol and acetone extracts by disk diffusion method with ten pothogenic fungi. Both the extracts showed moderate activity against Fusarium equiseti and Candida albicans. In this case, Grisofulvin was used as standard...
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Posted by admin on Apr 1, 2012 in |
Lipospheres of benzyl penicillin were formulated using the conventional thin film hydration technique. Five different combinations of shea butter, surfactant (Span 80) and goat fat were the key variables employed in the formulations. The resultant lipospheres were evaluated with respect to surface morphology, particle size distribution, encapsulation efficiency, in vitro drug release, in vivo bioavailability and in vitro antimicrobial activity. Particle size was found to increase with increased drug loading, the average particle radius of the batches being 12.5 nm. The encapsulation efficiency was found to be high at all levels with encouraging values of 80.21 and 83.44 % for batch A and E respectively, with batch A containing shea butter and span 80 in the ratio of 1:1 and batch E containing shea butter, span 80 and goat fat in the ratio of 1:2:1. Batch A appear to exhibit sustained release in vitro with cumulative drug release being 60 % while batch E has a cumulative drug release of 98 % respectively. The presence of goat fat however...
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Posted by admin on Apr 1, 2012 in |
Antimicrobial and cytotoxic activity of petroleum ether, ethyl acetate and methanol extracts from Juniperus indica Bertolwere evaluated. The extracts screened for their antibacterial activity against human pathogenic bacterial strains, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. All the fractions presented antimicrobial activity with variable strengths to all of the tested microorganisms. Phytochemical screening revealed the presence of terpenoids, flavanoids and tannins as main phytochemical groups. Cytotoxic activity was evaluated by using brime shrimp lethality test. LC50 value for the methanolic, ethyl acetate, and petroleum ether extracts were calculated at 550 µg/ml, 380 µg/ml and 140 µg/ml...
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Posted by admin on Apr 1, 2012 in |
Cinnamomum tamala is found in tropical and sub-tropical Himalayas and in some other places. Phytochemical screening of the plant extracts were done to screen their active constituents. Antimicrobial activity of this plant extract was investigated by Kirby-Bayer methodology against four pathogenic organisms: K. Pneumonae, E. coli, S. aureus, and P. aeroginosa. The plant Butanol extract showed inhibitory activity against all the tested organisms and it has enhanced immunomodulatory activity also. Butanol extracts containing remarkable positive results for phytochemicals compared to other solvent extracts. The higher concentration (100mg/ml) of butanol extracts shows maximum inhibition. Proliferation and activation of lymphocytes was also identified. The study scientifically validates the use of plant in traditional medicine....
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