Posted by admin on Jul 1, 2011 in |
Methanol extract of leaves of Spilanthes calva (DC) was examined for their antimicrobial and cytotoxic properties. To determine antibacterial activities, the extract was tested against four gram-positive and six gram-negative bacteria at three concentrations (500, 800, 1000 µg disc-1) through disc diffusion method. The extract showed moderate antibacterial actions and that was increased by increasing the concentration of the sample. The maximum antimicrobial potential was obtained against Staphylococcus aureus and no sensitivity was found for Escherichia coli. Comparatively gram-positive bacteria demonstrated more susceptibility to the extract than gram-negative bacteria. Cytotoxic property of the sample was done using Brine shrimp lethality bioassay where it did not show noticeable toxicity. So, our present study reveals that the leaves extract of S. calva possess considerable antibacterial properties along with lesser amount of...
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Posted by admin on Jul 1, 2011 in |
Antibacterial, antifungal and phytotoxic activities of the prepared pyrazine derivatives of benzil (A), 4, 4’-dibromobenzil (B) and α-diketones of triterpenoids skeleton e.g. 2, 3-diketolupene (C) and 2, 3-diketomethyl- dihydrobetulonate (D) were studied. The prepared 1, 4-pyrazine derivatives (E, F, G & H respectively) have been characterized by UV, IR, NMR (1H and 13C), optical rotation, mass spectra and by comparison with authentic samples. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Phytotoxicity was determined against seed growth technique. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The derivatives showed significant antimicrobial activities against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa (bacterial specimen), Colletotrichum camelliae, Fusarium equisiti, Alternaria alternata, Curvularia eragrostidis and Colletotrichum gloeosporioides (fungal...
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Posted by admin on Jul 1, 2011 in |
The aim of this study was to determine the antimicrobial activity of three selected Lebanese plants (Rheum rhaponticum, Olea europaea, and Viola Odorata) against Extended Spectrum Beta Lactamase (ESBL) – producing Escherichia coli and Klebsiella pneumoniae, and to identify the specific plant fraction responsible for the antimicrobial activity. The plants were extracted with ethanol to yield the crude extract which was further subfractionated by different solvents to obtain the petroleum ether, the dichloromethane, the ethyl acetate and the aqueous fractions. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined using broth microdilution. The MICs ranged between 2.5 and 80 μg/μl. The majority of these microorganisms were inhibited by 80 and 40 μg/μl of the crude extracts. The dichloromethane fraction of Olea europea exerted a significant inhibitory effect on 90% of the tested strains. Ethyl acetate extracts of all selected plants presented antibacterial activity with high potency. Aqueous extracts of Rheum rhaponticum and Olea europaea exerted antimicrobial activity against the majority of the tested strains while Viola...
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Posted by admin on Jun 1, 2011 in |
Premna integrifolia Linn. (Family: Verbenaceae) is a medicinal plant which is traditionally used against a number of diseases including inflammatory condition. On the scientific basis of traditional uses, the ethanolic extract of leaves of Premna integrifolia Linn. was assessed for its possible analgesic and antimicrobial activity. The analgesic activity of the sample was studied using acetic acid induced writhing model in mice. Antibacterial activity of leaves of Premna integrifolia Linn. was tested by using the disc diffusion method. In analgesic activity, the extract produced 52.17% (p<0.01) acetic acid induced writhing inhibition in mice at the dose of 500 mg/kg body weight, which is comparable to diclofenac sodium 65.21% (p<0.01) at the dose of 25 mg/kg body weight. The extract showed significant antibacterial activity against both gram positive and gram negative bacteria. All the results tend to justify the traditional uses of the plant and require further investigation to identify the chemicals responsible for these...
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Posted by admin on Jun 1, 2011 in |
Recently, heterocyclic compounds analogues and their derivatives have attracted strong interest in medicinal chemistry due to their biological and pharmacological properties. The small and simple benzothiazole nucleus possesses numerous biological properties like – antitumor, antimicrobial, anti-inflammatory, anticonvulsant, and antidiabetic activities. These activities are also possessed by its substituted derivatives as well. The present review focuses on some commonly used easy procedures to synthesize the benzothiazole moiety and its derivatives, which comprise of different biological...
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