Posted by admin on Mar 30, 2015 in |
The aim of this study was to determine principle phytochemical classes and total phenolic contents and to investigate antioxidant, anthelmintic and antimicrobial activities of ethanolic extract of Crataeva nurvala leaves. Standard test methodology suitable for each chemical class was followed to screen out major chemical classes preliminarily. The total phenolic content of ethanolic leaf extract was determined using Folin-Ciocalteu colorimetric assay. DPPH free radical scavenging assay and total reducing power test were carried out to investigate the antioxidant capacity. The conventional earthworm method and the Kirby-Bauer disc diffusion test were also performed to assess anthelmintic and antimicrobial activities of the extracts, respectively. Phytochemical screening of the ethanolic leaf extract showed the presence of significant amount of flavonoids and steroids along with other phyto-constituents in minor amount. Folin-Ciocalteu assay showed that 45.53 mg gallic acid equivalent was present per gram of the dried extract of the leaf. Leaf extract exhibited noticeable antioxidant activity in both of the DPPH free radical scavenging assay and the total reducing power test where ascorbic...
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Posted by admin on Mar 30, 2015 in |
The objective of this study was to evaluate the possible antimicrobial properties of water, methanol, chloroform and Petroleum ether extracts of leaves of different Indian medicinal plants including Mangifera indica Linn, Ixora parviflora Vahl, Psydium guajava Linn, Calotropis procera Ait. The extracts were investigated individually for in vitro antibacterial activity by cup plate (well diffusion) method against nine pathogenic bacteria cultures. Of all the plants investigated for antimicrobial potential, the leaf extracts of Psydium guajava showed high antimicrobial activity against the tested microorganisms. Methanolic leaf extract of Psydium guajava was found to exhibit highest antimicrobial activity. Water leaf extracts of Mangifera. Indica and Ixora parviflora did not show antimicrobial activity. Phenolic content of Psydium guajava could contribute towards its antibacterial activity. The antibacterial activities of the investigated plants could make it useful in microbial...
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Posted by admin on Mar 30, 2015 in |
Molybdenum is a trace element distributed widely in nature which plays an important role in plant and animal nutrition and in our industrial society. Molybdenum is essentially present in the enzymes nitrogenase, Xanthine-oxidase, sulphite oxidas, nitrate reductase, formate dehydrogenase and aldehyde oxidase. These enzymes are responsible for a number of catalytic reactions of redox nature. Molybdenum complexes were synthesized and characterized for their purity by various physico-chemical methods like IR, Electronic, 1H1 NMR spectral studies Antimicrobial activity of compounds against certain microbial species has been carried out by serial dilution method using BOD incubator to evaluate the MIC (Minimum Inhibitory Concentration) value on the basis of MIC value it is interpretated that Molybdenum complexes are most active as compared to ligand fragments. The DNA binding ability of the complexes was examined by using biophysical spectroscopic studies. These studies indicate that the compounds can intercalate and stablize DNA. This work has great importance in the field of bioinorganic...
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Posted by admin on Feb 28, 2015 in |
In-vitro antibacterial and antifungal activity of Tridax procumbens plant parts (leaf and stem) were investigated by agar well diffusion method. The petroleum ether, methanol and aqueous extracts of the plant parts were tested against two bacterial strains i.e., Bacillus subtilis and Escherichia coli and two fungal strains i.e., Trichoderma reesei and Fusarium oxysporium. The results of antibacterial activity showed that methanol extract was effective and aqueous extract was devoid of any significant activity. Methanol extract of Tridax procumbens showed maximum antifungal activity. The results lend credence to the folkloric use of this plant in treating microbial infection and showed that Tridax procumbens leaves and stem could be exploited for new potent antimicrobial...
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Posted by admin on Feb 26, 2015 in |
A series of novel 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl-amino)-4-(substituted phenyl)azetidin-2-one analogues (3a-e) were synthesized starting from 2-thiouracil. Reaction of 2-thiouracil with 4-fluorobenzylchloride gave 2-(4-fluorobenzylthio) pyrimidin-4(3H)-one which on chlorination with POCl3 yielded 2-(4-fluorobenzylthio)-4-chloropyrimidine. Further reaction of compound 2-(4-fluorobenzylthio)-4-chloropyrimidine with hydrazine hydrate generated the key intermediate 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl)hydrazine (1). Reaction of compound 1 with appropriate substituted aromatic aldehydes furnished 2-(substituted benzylidene)-1-(2-(4-fluorobenzylthio) pyrimidin-4-yl)hydrazine (2a-e), which on further cyclisation with acetyl chloride in dry benzene furnished 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl-amino)-4-(substituted phenyl) azetidin-2-one analogues (3a-e). Structural assignments of the synthesized compounds were based on their IR, 1H NMR, Mass and analytical data. All the synthesized compounds 3a-e were screened for their preliminary antimicrobial properties. Some of the compounds exhibited promising antimicrobial...
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