Posted by admin on Aug 31, 2020 in |
The aim of the present research work was to develop a bilayer floating dosage form of immediate-release Atorvastatin (ATR) and sustained release Captopril (CPT) in matrix form for the treatment of hyperlipidaemia and hypertension to reduce multiple dosing frequencies and enhance patient compliance. ATR belongs to the BCS Class II category having poor aqueous solubility, which was enhanced by using hydrophilic polymer PEG 8000 by kneading technique. A sustained layer of CPT was prepared by melt granulation technique using various concentrations of carnauba wax with HPMC K 100 M and Carbopol 934 P. All the parameters before and after compression were evaluated. ATR-PEG ratio of 1:0.25 showed a higher drug release of about 97.42% within 60 min. The optimized CPT sustained layer SR2 shown the highest drug release 98.2% in 12 h. In-vitro drug release studies carried out as per USP in pH 1.2 buffer using type II apparatus. The CPT layer exhibited release kinetics in the first order and followed the diffusion and erosion mechanism. The FTIR, XRD...
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Posted by admin on Jul 31, 2020 in |
Quality by Design (QbD) is a systematic approach for the development of products and processes. Azilsartan Medoxomil is a competitive antagonist of the Angiotensin II Type 1 receptor. A precise and reproducible ultra-performance liquid chromatographic (UPLC) method was developed and validated for the estimation of Azilsartan Medoxomil from its marketed tablet dosage form applying Quality by Design approach. Eight experiments were conducted using the full factorial design in order to rationally examine the effects of stationary phase, buffer pH and organic phase concentration in primary screening followed by method optimization with variation in Column temperature and flow rate. Chromatographic separation of AZIL was achieved at 40 °C temperature using an Acquity UPLC BEH C18 (4.6mm × 2.1mm, 1.7μ) analytical column; the mobile phase consisted of Acetonitrile: phosphate buffer (pH 5.5, 0.02mM) (40:60, v/v) at a flow rate of 0.3 ml min-1. Azilsartan eluted with retention time (Rt value) 2.41 min. The linear regression analysis showed good linear relationship with a correlation coefficient of 0.999. The limit of detection and...
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Posted by admin on Jun 30, 2020 in |
Bombax ceiba is a traditional medicinal plant that is beneficial in the treatment of diuretic, hemostatic, antidysenteric, emetic, antipyretic, astringent, and commonly known silk cotton tree, semal, kapok tree. The aim of this study is to isolate and characterize the biologically active compounds from the ethanolic extract of the leaves. The EtOH extract was subjected to silica gel (60-120 mesh) by glass column chromatography, using gradient petroleum-ether, C6H6, CH3OAc, CH3OH, and purified by preparative TLC (GF254) to yield two pure natural products (taraxerol and β-sitosterol glucoside). The structure was elucidated on the basis of spectroscopic analysis, including 1HNMR, 13CNMR, DEPT-135, COSY, HSQC, and HMBC. The β-sitosterol glucoside shows pharmacological activities such as lowering cholesterol levels, reducing blood pressure, antitumor activity, etc. The glucose moiety is highly effective because it is hydrophilic, which inhibits the entry of cholesterol into the esterification of cholesterol. It is the first time isolated from the leaves of this...
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Posted by admin on May 31, 2020 in |
Dieffenbachia amoena is a house plant and known dumb cane. The study was performed based on isolation and structure elucidation of phytosterols from the extract of this plant. The CH3OH crude extract was loaded over the silica gel (60-120 mesh) column, using the stepwise gradient C6H6, CH3OAc, CH3OH. The fractions were further purified by preparative TLC (GF254) to yield compound (1). Compound (1) was characterized by using various standard spectroscopic techniques such as IR, 1HNMR, 13CNMR, DEPT-135, COSY, HSQC and HMBC. Based on the spectral analysis, it was confirmed that the compound (1) was a mixture of β-sitosterol and stigmasterol. Phytosterols have high medicinal importance and play a vital role in reducing blood cholesterol, a high level of blood cholesterol can cause a risk of cardiovascular disease. Cardiovascular disease is the main problem of the whole world and increasing day by day. We have isolated β-sitosterol and stigmasterol first time from the leaves of the Dieffenbachia amoena...
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Posted by admin on Nov 30, 2019 in |
Depression is the most common and spreadable disorder in recent age as people are going through a lot of pressure in their daily work caliber and so many unconditional events. A new approach called derivatization spectroscopy was developed for the anti-depressant agent, marketed formulation of Bupropion hydrochloride, to confirm the interfering substances from the excipients of solid dosage form, in addition to that the stability parameters to assure the analysis time with the minimum usage of the reference standard and sample solution. The present work aims to develop simple, sensitive stability indicating the method to develop a model to estimate BPH in the solid dosage form by using derivative spectroscopy tool to avoid the excipients interferences in the developed assay method. First, made the stock solution of 100 µg/ml from 1000 µg/ml previously prepared solution. The method was validated over the concentration range of 5-50 µg/ml of marketed formulation of Bupropion Hydrochloride. The assay method & derivatization was performed using UV spectroscopy at 252 nm wavelength. The intra-assay &...
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