The present study investigates neuroprotective effect of hydroxy citric acid (50%) on phenytoin induced cognitive impairment and oxidative stress in rats.Male Wistar rats were divided into 6 groups and each group received vehicle, phenytoin (25mg/kg, i.p) and hydroxy citric acid (400 and 800mg/kg, orally) for 14 days respectively. Memory and learning was evaluated by using the Rectangular maze, Mo...
Strychnos potatorum Linn. is a medium sized deciduous tree belonging to the family Loganiaceae. The collected seeds of Strychnos potatorum were dried and powdered. Methanolic extract of these seeds was prepared by cold maceration. The preliminary phytochemical screening of the crude extract revealed the presence of flavonoids and phenolic compounds. The crude extract was partitioned (gradient) wit...
QbD BASED RP-HPLC METHOD DEVELOPMENT FOR FIVE FLUOROQUINOLONE ANTI-BACTERIALS – THROUGH CREATION OF DESIGN SPACE FOR CRITICAL ATTRIBUTES
Quality by Design (QbD) has become an important concept for the pharmaceutical industry, and ICH recommends this concept through the Guideline Q8 (R2) wherein if the method is proved to be rugged and robust within the limits of a Design Space then approval may be requested for such a Design Space, and the method does not need redevelopment, revalidation, and reapproval during transfers, thereby sa...
A STUDY TO EVALUATE THE PATTERN OF PRE ANAESTHETIC MEDICATION IN VARIOUS SURGICAL SPECIALTIES IN A TERTIARY CARE HOSPITAL
Objective: To analyse and compare pattern of pre anaesthetic medication use in various surgical specialties. Methodology: Data was collected retrospectively from inpatient records of major surgeries in the Department of Surgery, Orthopaedics, Neurosurgery, Paediatric Surgery, Obstetrics and Gynaecology, Plastic surgery, Urology and Vascular Surgery at M.S. Ramaiah Hospitals, during the period o...
ALPHA-AMYLASE INHIBITION AND MEMBRANE STABILIZING EFFECT OF THE STEM BARK OF MAESOBOTRYA DUSENII HUTCHINSON
Introduction: Control of blood glucose level is a critical approach in the management of diabetes mellitus and its complications. This study investigated the α-amylase inhibition and erythrocyte membrane stabilization effect of extracts of Maesobotrya dusenii Hutch. Method: Powdered stem bark of M. dusenii (100g) was extracted with methanol. The plant material (400g) was also successively macerat...
The studies on the constituents of aerial parts of Stellaria media Linn. (Caryophyllaceae) have led to the isolation and characterization of three new compounds ─ (1), (2) and (3). Structure elucidation of the new compounds was carried out using 1H NMR, 13C NMR, Mass spectrometry together with other complementary techniques (UV and IR). The major findings of the study include: Compound 1 was sep...
FORMULATION DEVELOPMENT AND EVALUATION OF FENOFIBRATE AND ROSUVASTATIN IN COMBINATION TABLETS AND RELEASE RATE IMPROVEMENT
The work was aimed to develop an immediate release tablet formulation of Fenofibrate and Rosuvastatin in combination for better treatment as well as management of hypercholesterolemia and prevention of cardiovascular diseases. The target was also set to improve the dissolution profile of poorly soluble Fenofibrate. For manufacturing these immediate release combined formulation tablets, Hot-Melt Te...
Administration of ethanol extract of Polygala chinensis whole plant (100 mg/kg and 200 mg/kg body weight) to alloxan induced diabetic rats for 14 days reduced the elevated level of lipid peroxidation (LPO). The treatment also resulted in significant increase in reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) in serum, liver and kidney. The res...
Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitor...
TWO NEW METABOLITES; 7β-OH-UZARIGENIN AND 7β-OH-ODOROSIDE-H; THROUGH BIOTRANSFORMATION OF UZARIGENIN AND ODOROSIDE-H
Two new cardenolide metabolites, 7β-OH-uzarigenin (1M) and 7β-OH-odoroside-H (2M) were obtained through the biotransformation of uzarigenin (1) and odoroside-H (2). Compounds 1 and 2 were isolated from the air-dried roots of Nerium oleander L. The plant, belonging to family Apocynaceae, was collected in October, 2010 from Mansoura university garden in Egypt. The methylene chloride extract of the...