Posted by admin on Aug 31, 2015 in |
This paper presents the synthesis and physical-chemical characterization of a new terpyridine (tpy) ruthenium complex coordinated to the L-type calcium blocker channels verapamil (vera) obtained from commercial pill. Elemental analysis (%C N H), infrared (IR) and ultraviolet-visible (UV-vis.) spectroscopy, mass spectrometry (MS), and 13CNMR (nuclear magnetic resonance), and HPLC (high performance liquid chromatography) confirmed the pure and structure of the ligand verapamil and of the ruthenium complex structurally designed as [RuIICl2(tpy)(vera)] Cyclic voltammetry aided investigation of the electrochemical behavior of [RuIICl2(tpy)(vera)] based in the metal ion redox potential. Verapamil ligand and [RuIICl2(tpy)(vera)] induced vascular relaxation in endothelium-intact and endothelium-denuded aortic rings. Verapamil drug and [RuIICl2(tpy)(vera)] complex coupled to verapamil elicited similar pharmacological results. The calcium ion chelating effects (Kd) promoted for verapamil ligand and [RuIICl2(tpy)(vera)] complex were found based in the fluorescence technic. The smooth muscle cell images studied to [RuIICl2(tpy)(vera)] complex acting as a luminescent agent important as biological...
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Posted by admin on Aug 31, 2015 in |
This study was carried out to evaluate the effect of boiling on amino acids contents of the fruits of four species of the family Cucurbitaceae which were Cucurbita maxima D, Cucurbita pepo L, Cucurbita moschata and Lagenaria siceraria variety supreme court. In this study, fresh fruit of the four species were subjected to conventional processing technique which is boiling as well as drying. The dry and boiled fruits were evaluated for essential and nonessential amino acids content as well as total amino acids. Amino acids profile of boiled and dry fruits showed the presence of eight essential and nine non essential amino acids. The four species recorded a considerable amount of proline as follows: L. s. uncooked (370 mg/100gm), C. mo. uncooked (318 mg/100gm) L. s. cooked (258.8 mg/100gm), C. p. uncooked (232mg/100gm) and C. p. cooked (208mg/100gm). The highest total amount of amino acids of boiled fruits was recorded in Lagenaria siceraria (4977.6mg/100gm) followed by Cucurbita pepo (2870.1mg/100gm) where as the lowest was recorded in Cucurbita maxima (1427mg/100gm). ...
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Posted by admin on Aug 1, 2015 in |
Present investigation deals with the phytochemical and antioxidant studies of Justicia gendarussa Burm.f. (family Acanthaceae), a medicinally important herb. Biochemical studies highlighted that in leaf tissue, quantities of soluble carbohydrate, amino acid and ascorbic acid are of 307 mg/g, 34.93 mg/g and 619µg/g, respectively. In stem bark, quantities of soluble carbohydrate, amino acid and ascorbic acid are of 185 mg/g, 26.86 mg/g and 330.33µg/g, respectively. Pigment content in dried leaf has been estimated as chlorophyll a 46µg/g, chlorophyll b 16.1µg/g and total carotenoids 16.7µg/g. In bark, amounts of chlorophyll a, chlorophyll b and total carotenoids are of 23.9µg/g, 21 µg/g and 14.6µg /g, respectively. Enzyme content in fresh leaf showed that amount of the catalase is 4.89 EU/ g fresh tissue and in bark it is 2.39 EU/ g. Peroxide is 3.4 EU/g for fresh leaf and 2.21 EU/g in stem bark DPPH radical scavenging activity was considerably high for methanolic extract of leaf that is 31.36 % and in case of bark the activity was 23.74 %. The...
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Posted by admin on Jul 31, 2015 in |
Methylmercury (MeHg), a highly toxic environmental pollutant, induces oxidative stress and dysfunction in many cell types. Bacopa monniera is a perennial herb, and is used as a nerve tonic in ayurveda, a traditional medicinal system in India. This study was aimed to evaluate the effect of Bacopa monniera extract (BME) on MeHg-induced toxicity in rat cerebellum. Male Wistar rats were administered with MeHg orally at a dose of 5 mg/kg b.w. for 21 days. Experimentalrats were given MeHg and also administered with BME(40 mg/kg, orally) 1 h prior to the administration of MeHg for 21 days. After treatment MeHg induction caused increased level of Scgb3a1mRNA whereas the level was reduced by BME pretreatment. GPx4 expression was down regulated and HSP70 expression was up regulated in MeHg intoxicated group. Immunohistochemical changes were observed in TRxR1 and GFAP protein in MeHg induced group. BME pretreatment altered the expression of proteins and Immunohistochemical changes to near normal. These findings strongly implicate that BM has potential to protect brain from oxidative damage resulting...
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Posted by admin on Jul 31, 2015 in |
The present study aims to explore the ethnomedicinal knowledge of the plants and their preparations used to cure diabetes by the tribes of Mananthavady, Bathery and Vythiri taluks of Wayanad district, Kerala. Preparation of ethnomedicine using various plant parts and other information related to tribes associated; habitat, habit and status of medicinal plants recorded are highlighted. Documentation of indigenous knowledge related to cure diabetes was made by interaction with traditional healers. A total of 23 species belonging to 17 families possessing antidiabetic property were recorded. The review of literature revealed that 16 species have already been proven to be antidiabetic through scientific analysis. However reports on antidiabetic properties of Andrographis wightiana T. Anderson & Thwaites, Psilotrichum elliotii Baker & Clarke, Solanum violaceum Ortega ssp. multiflorum (Clarke), Justicia trinervia L., Dracaena terniflora Roxb., Elaeagnus conferta Roxb. and Kydia calycina Roxb. are lacking. The present study is successful in identifying seven medicinal plants used by the tribes for possessing antidiabetic activity. Most of the herbal preparations for curing diabetes were prepared...
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