Posted by admin on Sep 30, 2015 in |
Aim: Hypothyroidism is one of the commonest metabolic diseases which afflicts females more than males and is often associated with myopathy. Reports on relationship of muscle-markers with thyroid hormones in the (a) euthyroid population in general and (b) hypothyroid population in India are scarce. Materials and Methods: A randomized case-control study on the female population for which 60 hypothyroids and 30 euthyroid controls were selected. Total triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH) and two muscle markers – lactate dehydrogenase (LDH) and creatine kinase (CK) were measured from serum samples using automated analyzers. Results: CK was 71% higher and LDH 23.5% higher in hypothyroids than in euthyroid controls. CK correlated well with T4 in hypothyroids (r= – 0.68, p<0.0001) and T3 in euthyroids (r= 0.62, p= 0.003) but to a lesser extent with others viz. T3 in hypothyroids (r= -0.46, p= 0.0002), T4 in euthyroids (r= -0.49, p= 0.007) and TSH both in hypothyroids and euthyroids (r= 0.43, p= 0.0006; r= -0.40, p= 0.03 respectively). LDH correlated weakly...
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Posted by admin on Sep 30, 2015 in |
Cadmium is a potent neurotoxic heavy metal, which induces oxidative stress and membrane disturbances in brain. Oxidative stress has been implicated in the pathology of number of neurodegenerative disorders including Alzeimers disease. The present study was aimed to evaluate the antioxidant effect of hexane extract of Citrullus vulgaris seed (CVSO) in the brain of cadmium (cd) treated mice as an animal model of oxidative stress. The neuroprotective efficacy of CVSO against Cd was assessed by estimating the levels of lipid peroxidation, non-enzymatic antioxidant, enzymatic antioxidant status and acetylcholinesterase (AChE) activity in mice. CVSO was administered along with Cd for four weeks. The results showed that mice intoxicated with cadmium (5 mg/kg/day) for 4 weeks significantly (p<0.05) reduced the AChE levels in brain, elevated the levels of thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides along with the significant (p<0.05) decrease in the levels of non-enzymatic antioxidants (GSH), enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in the brain tissue. In conclusion, CVSO ameliorated cadmium induced brain damage by...
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Posted by admin on Sep 30, 2015 in |
The Human immunodeficiency virus(HIV)type-1 integrase is one of the most important target of highly active anti retrovirus therapy (HAART), due toits role to incorporate genetic information into the host DNA, so its prevention to its proper function results in very fine therapeutic effect for the treatment of all acquired immune deficiency syndrome(AIDS), extensive research work on integrase inhibitors(INIs) haven’t carried out till present due to complexities in research with integrase and a very few drug are known to inhibit integrase. Dolutegravir is a new 2nd generation Integrase inhibitor (INIs) in a short list of INIs, recently approved by FDA in the list of HAART, so herein we taken Dolutegravir as a reference structure for virtually identification of more/similar efficient drug like leads then Dolutegravir using three different PDB structures (4S3O, 3S3M & 3S3N) of Integrase having in different mutated state from PDB database ‘RCSB’ versus chemical compounds database ‘ZINC’ using Schrodinger and Discovery Studio software. Using molecular constraint search with similarity coefficient ‘Tanimoto’, 1,65,000 ligands were extracted out and...
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Posted by admin on Sep 30, 2015 in |
The present research was focused on the development of an oily suspension for oral administration of omeprazole in Pediatrics. Currently, for the treatment of this population, extemporaneous preparations are used in hospitals with many limitations in their use as bad organoleptic features and loss of drug activity due to improper manipulation, causing the omeprazole to be sensitive to the acidic environment of the stomach, with the consequent loss of activity. The objectives are to achieve a stable formulation of omeprazole in normal storage conditions and to ensure that this formulation maintains the active ingredient protected from the hostile environment of the stomach, which causes degradation of the drug. An oily omeprazole suspension was elaborated and compared to three master formulas usually employed in hospitals (pellets suspension, xanthan suspension, Ora-Sweet®suspension). The omeprazole suspension has been characterized as follows: appearance and organoleptic characteristics, content uniformity test, viscosity, study of dissolution rate and formulation...
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Posted by admin on Sep 30, 2015 in |
Nigella sativa (NS) is a commonly used herb in Asian and African countries. Extensive experimental studies have been done to shed light on its hepatoprotective, immunumodulator, antioxidant, antimicrobial and several other useful pharmacological properties. However, there is dearth of evidence to evaluate the effect of Nigella sativa extract in neuropathic pain. In this study we have used neuropathic pain model of Wistar rats using intraperitoneal cisplatin injection twice weekly for 4.5 weeks. Animals were divided into six groups of six rats each. Ethanolic extract of Nigella sativa, pregabalin, tramadol or combination of pregabalin and tramadol were given orally in Wistar rats in which neuropathy had been induced by cisplatin i.p. injections for 4.5 weeks. Single dose of drugs were given and pain assessment was done by soft touch, crude touch, Eddy’s hotplate and tail flick analgesiometer at 0, 30, 60, 90, 120 and 240 minutes. Nigella sativa showed significant analgesic effect on cisplatin induced neuropathic pain in all the experiments however pregabalin and tramadol showed better...
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