Posted by admin on Nov 30, 2015 in |
Introduction: Green tea contains epigallocatechin gallate (EGCG) that widely use as antibacterial. Formulation development with buffer and emulsifier addition is needed to increase its stability and effectiveness. Buffer addition will increase EGCG stability while emulsifier will increase the cream stability. Objective: This study aimed to determine the optimal formulation from buffer combination between citric acid, ascorbat acid, and triethanolamine that produce pH 5 and also emulsifier combination from propylene glycol, tween 80 and span 80 using simplex lattice design method. Moreover, the effectiveness of optimum cream formula was tested against Propionibacterium acnes (P.acnes) and Staphylococcus epidermidis (S.epidermidis) while its sensitivity was tested on rabbit’s skin. Methods: Buffer optimization was obtained using 13 compositions with the combination of citric acid, ascorbat acid, and triethanolamine namely: 100-0-0,0-100-0,0-0-100,50-0-50,50-50-0,0-50-50,33-33-33,1,67-1,67-6,67, 6,67-1,67-1,67, 1,67-6,67-1,67, 100-0-0,0-100-0,0-0-100. Data was analyzed using design expert. The optimum buffer combination from ethyl acetate fraction of green tea leaves extract then formulated in the cream which contained various emulsifier composition. The comparison from 13 emulsifier composition of propylene glycol, tween 80, and...
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Posted by admin on Nov 30, 2015 in |
Ficus benghalensis is has been used in traditional medicinal practices such as Ayurveda, Unani, Siddha and homoeopathy. All parts of the tree such as fruits, aerial roots, bark and leaves are known to be traditionally associated with diarrhea, dysentery, menorrhagia, nervous disorders and tonic properties. However, in Ayurveda it is mainly known for the diabetes treatment.There are no reports giving a detailed profiling of phytochemicals present in the aerial roots of F. benghalensis. The study aims to evaluate its bioactive compounds and comprehend its biological activities with traditional knowledge.Methanol and ethanol extracts from the aerial roots healthy tree of F. benghalensis were prepared and their antibacterial activity against various pathogenic gram’s positive and gram’s negative bacteria were evaluated using disc diffusion assay. DPPH & FRAP bioassays were performed to establish its traditional use as antioxidants. Total phenol and total flavonoids were estimated to correlate its antioxidant potential. The chemical composition of methanol extract was accomplished by GC-MS and bioactivity of these compounds was determined. Lupenyl acetate (35.4 %), α-Amyrenyl...
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Posted by admin on Nov 30, 2015 in |
The antioxidant activities of five wild edible fruits e.g. e.g. Debregeasia longifolia, Helicia erratica, Ilex venulosa, Rhus semialata and Spondias axillaris collected from Meghalaya state in India were determined by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. The solvent systems used were benzene, chloroform, acetone and methanol. The different levels of antioxidant activities were found in the solvent systems used. The results indicate that these wild edible fruits could be utilized as natural...
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Posted by admin on Nov 30, 2015 in |
The study presents a simple and rapid chromatographic technique for the estimation of chlorhexidine gluconate for mouth wash using a combination of phosphate buffer and acetonitrile as mobile phase. The separation was carried out on a stainless steel column 250mm × 4.5mm packed with octadecyle silane particles. The detection was carried out using a dual lambda absorbance detector at 254nm wavelength. The method is found to be linear in the concentration range 10.56µg/ml to 98.56µg/ml and correlation coefficient 0.998. The analyte peak was found to be symmetric and the average retention time is 2.919mins. The method was found to selective, precise and accurate for the quantification of the drug from mouth rinse available in the...
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Posted by admin on Nov 30, 2015 in |
A preliminary experiment was carried out to analyse the growth performance of Agrocyba aegerita mushroom cultures using chemosynthetic media. This study is mainly aimed to different media such as Yeast Malt Agar Medium (YMA), Saboraud’s Dextrose Agar Medium (SDA), Glucose Peptone Agar Medium (GPA), Malt Extract Agar Medium (MEA) and Potato Dextrose Agar Medium (PDA) on the growth of mycelium of the mushroom. Studies revealed that the joint portion of cap and stripe produced vigorous mycelium growth in minimum time; the average maximum growth was obtained on Malt Extract Agar (MEA) than Potato Dextrose agar (PDA)...
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