Posted by admin on Aug 31, 2015 in |
Objective: To evaluate hypolipidemic potential of Kasni (Cichorium intybus) seed preparations combined with oral hypoglycemic agent in treatment of patients of newly diagnosed type 2 DM. Methods: Newly diagnosed 90 patients of Type2DM, age 35-65years, of either sex were divided into 3 groups. Each group having 30 patients (19 male and 11 females) matched with each other in terms of age and sex. In group I only Glycomet SR once a day and in group II/III 6 grams crude seed powder or 50 ml decoction of crude seed powder twice a day in combination with Glycomet SR once in a day, was given for 90 days. Serum cholesterol, triglyceride, LDL and HDL levels were measured at zero, 30th, 60th and 90th day. Results: All the three groups showed a significant reduction in cholesterol, triglyceride and LDL across the four time periods. Post hoc Tukey HSD test shown that there was a significant difference between group I & II (p=0.045) and group I & III (p=0.000) for cholesterol; group I...
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Posted by admin on Aug 31, 2015 in |
The solid microemulsion of Darunavir, a promising antiretroviral drug, is attempted in the present work for enhancing the solubility and dissolution rate. For this purpose, solubility of Darunavir was determined in various oils, surfactant and cosurfactants. Pseudo-ternary phase diagrams were constructed to identify the microemulsion existing zone. The optimized formulation consisted of 65% of Capmul MCM, 20% of Cremophore RH 40: Transcutol P (1:1) and distilled water. The optimized microemulsion formulation was characterized for its refractive index, % transmittance, pH, viscosity, drug content and particle size. Particle size of the optimized microemulsion formulation was found to be 40.68 nm. Various adsorbents were incorporated in the optimized liquid microemulsion to get solid microemulsion. The solid microemulsion with aerosil 200 was optimized because of very low concentration (1:1) of aerosil was incorporated in microemulsion as compare to other adsorbent. The prepared solid microemulsion was subjected to characterization for angle of repose, bulk density, tapped density, Hausner’s Ratio, Carr’s index, drug content, ex-vivo drug release study and stability studies. The solid microemulsion...
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Posted by admin on Aug 31, 2015 in |
A novel, simple, rapid and sensitive UV spectrophotometric method has been developed for simultaneous estimation of Gemcitabine and Tamoxifen. Gemcitabine and Tamoxifen show maximum absorbance at 269 nm and 238 nm respectively. The method employs first order derivative method. Here in this method, TAM show zero absorbance i.e Zero Crossing Point (ZCP) at 285 nm and at this wavelength GEM show measureable absorbance and ZCP of GEM is 224 nm and at this wavelength TAM show measurable absorbance. So absorbance of GEM and TAM is measured at 285 nm and 224 nm respectively. Both the drugs obey Beer’s Law in the concentration ranges employed for this method. R2 value of GEM and TAM was found to be 0.996 and 0.998 respectively. Accuracy and reproducibility of the proposed method was statistically validated by recovery studies. Limit of detection and (LOD) and limit of quantification (LOQ) of GEM was found to be 0.02833 and 0.08586 respectively. LOD and LOQ of TAM was found to be 0.09565 and 0.2898 respectively. The method...
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Posted by admin on Aug 31, 2015 in |
A simple, economical, specific, accurate, precise and validated Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of Phenylepherine hydrochloride (PPEH) and Cetirizine hydrochloride (CETH) in combine dosage form. The chromatographic separation was achieved on Princeton SPHER C18 column (250 mm x 4.6 mm id, 5 μ particle size) at ambient temperature using mobile phase buffer (0.1 M Ammonium dihydrogen phosphate pH 5.2 ± 0.05) : Acetonitrile (50:50% v/v) at flow rate 1.0 ml/min. Quantification was achieved with UV detector at 225 nm. Retention time of Phenylepherine hydrochloride and Cetirizine hydrochloride were found to be 2.19 ± 0.05 minute and 4.16 ± 0.05 minute respectively. Linearity was studied in the concentration range 5-30 μg/ml and 10-60 μg/ml for Cetirizine hydrochloride and Phenylepherine hydrochloride respectively with a correlation coefficient of 0.9998 and 0.9998 respectively. The proposed method was validated according to ICH guidelines with respect to linearity, accuracy, precision, robustness, LOD, and LOQ. The developed method with good separation, successfully applied for determination of PPEH...
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Posted by admin on Aug 31, 2015 in |
This paper presents the synthesis and physical-chemical characterization of a new terpyridine (tpy) ruthenium complex coordinated to the L-type calcium blocker channels verapamil (vera) obtained from commercial pill. Elemental analysis (%C N H), infrared (IR) and ultraviolet-visible (UV-vis.) spectroscopy, mass spectrometry (MS), and 13CNMR (nuclear magnetic resonance), and HPLC (high performance liquid chromatography) confirmed the pure and structure of the ligand verapamil and of the ruthenium complex structurally designed as [RuIICl2(tpy)(vera)] Cyclic voltammetry aided investigation of the electrochemical behavior of [RuIICl2(tpy)(vera)] based in the metal ion redox potential. Verapamil ligand and [RuIICl2(tpy)(vera)] induced vascular relaxation in endothelium-intact and endothelium-denuded aortic rings. Verapamil drug and [RuIICl2(tpy)(vera)] complex coupled to verapamil elicited similar pharmacological results. The calcium ion chelating effects (Kd) promoted for verapamil ligand and [RuIICl2(tpy)(vera)] complex were found based in the fluorescence technic. The smooth muscle cell images studied to [RuIICl2(tpy)(vera)] complex acting as a luminescent agent important as biological...
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