Posted by admin on Jun 30, 2015 in |
Workers engaged in tyre retreading face the possibility of occupational exposure to benzene and toluene as these are used as solvents and glue. Exposure to these solvents in other industries has been aptly described in several reports. Considering important from public health point of view, a study on biological monitoring of benzene and toluene exposure in tyre retreaders was performed Urine samples were analyzed for the metabolites of benzene and toluene viz. phenol and hippuric acid respectively. The information like smoking, alcohol intake, age and duration of job was sought after personal interviews. Results showed, that population of smokers excreted less phenol but high hippuric acid in comparison to non smokers. Alcohol intake inhibited the metabolism of benzene and toluene. Excretion of phenol and hippuric acid was lower in alcoholics than non alcoholics. Age also affected the excretion of phenol. It was found to be lowest in the age group of 30-40 years. Excretion of phenol was lowest in workers having the work experience of 16-20 years, whereas, excretion...
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Posted by admin on Jun 30, 2015 in |
Zopiclone belongs to a class of medicines commonly called Z-drugs. It is a novel hypnotic agent used in the treatment of insomnia. Its mechanism of action is based on modulating benzodiazepine receptors. A Reverse Phase High Performance Liquid Chromatography method was developed and validated for the quantification of zopiclone in bulk and tablet dosage form. Chromatography was achieved using a Waters X Bridge C18 column (50 x 2.1 mm, 5μ) eluted with a mixture of mobile phase composed of ammonium bicarbonate (5mM, neutral) and acetonitrile in a gradient mode at a flow rate of 1 ml/min. The detection of eluent from the column was detected using photo diode array detector (PDA) at 214nm. The stability indicating assay method was developed and validated as per the ICH guidelines using the required parameters. Linearity was observed within the concentration range of 50-300μg/ml with coefficient of correlation 0.999. The LOD and LOQ were 9.416μg/ml and 28.534μg/ml, respectively. The mean recovery was found to be...
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Posted by admin on Jun 30, 2015 in |
Female sexual dysfunction is a complicated disorder and mostly ignored. Worldwide, reduced sexual function occur in all ages but largest reduction is seen in menopausal women. Now, in the market various conventional medicines are available but they have various side effects and are less efficacious. Therefore the scientists diverted towards the medicinal plants claimed in the traditional systems of medicines. Withania somnifera, one of the plants from Ayurvedic medicinal system has been traditionally claimed to enhance the sexual activity in male and female. In females, it has not been scientifically documented like study on male sexual dysfunction. So, in the present study hydroalcoholic extract of dried roots of Withania somnifera (100, 200 and 300mg/kg/day, p.o. for 21 days) was investigated for increase in sexual activity in tubal ligated female rats (150-200g). Readings for sexual behaviours were taken on 11th and 21th day by using automated runway and Copulatory arena apparatus. At the end of the study female rats were decapitated, blood samples were collected for estimation of serum estradiol...
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Posted by admin on Jun 30, 2015 in |
Lysosomes of mammalian tissues contain a number of cysteine proteinases such as Cathepsins B, H and L, which participate in intracellular protein degradation and are involved in various pathological disorders. These proteinases have been purified to ~ 1600 fold with ~25% yields from goat liver. These Cathepsins having similar molecular weight (~25,000 Da) were obtained as a single pool after initial purification steps i.e. homogenization of acetone powder, acid autolysis at pH 4.0, 30-70 % (NH4)2SO4 fractionation and molecular sieve chromatography on Sephadex G-100, were simultaneously separated and purified on exchange chromatographies. Cathepsin L was completely separated and purified from Cathepsin B & H at CM- Sephadex C-50 column at pH 5.6 and was eluted as bound protein at 0.62 M NaCl with a purification fold ~1629 and 22 % yield. Cathepsin B was eluted as bound protein in ~1545 fold with ~45% yield at 0.40 M concentration. Complete separation of Cathepsin H from Cathepsin B was achieved on DEAE-Sephadex A-50 at pH 6.0 where the former was obtained...
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Posted by admin on Jun 30, 2015 in |
Present era meant for utilization of natural resources in the development of dosage forms. In the present investigation natural biodegradable polymer olibanum resin is employed to develop matrix tablets of ambroxol hydrochloride for sustained release. Various Ratios of resins is study its ability to retard the early release of the drug from formulation. The tablets were prepared by wet granulation technique and were subjected for their thickness, weight variation test, drug content, hardness and friability. The in vitro dissolution studies were carried out using USP apparatus type II (paddle) at 100 rpm. Drug content of the formulations ranged between 97.78±0.08 to 100.12, where as hardness varies from 5.9-7.6kg/cm2. From the in vitro release studies it was found that F5 formulation was similar to marketed sample hence it was selected as a optimized formulation. Higuchi release kinetics shows r2=0.9298, from Korsemeyer- Peppas plot n value >0.89 indicates super case II type of release indicating that the drug release from these formulations is by erosion of polymer. The optimized formulation of...
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