Posted by admin on Aug 31, 2015 in |
The current study focuses on evaluating chemical composition and antimicrobial activity of seed oil of Broussonetia papyrifera; a widely used Chinese medicinal plant. The chemical constituents of the oil were evaluated by GC‑MS analysis. Antimicrobial activity and minimum inhibitory concentrations were determined by disk diffusion and agar dilution methods. The seed oil contains 46 different phytoconstituents where major compounds were hexadecanoic acid (43.6%), heptadecene‑8‑carbonic acid (17.5%) and caryophyllene (8.4%). Seed oil exhibited inhibitory effect on Staphylococcus aureus, Proteus vulgaris, Bacillus cereus, and Enterobacter aerogenes, while it showed no such inhibitory effect on fungal strains. The studies revealed that majority of the compounds in oil were saturated fatty acids and their derivatives. The oil possessed significant antibacterial activity against selected bacterial strains while tested fungal strains were completely resistant to B. papyrifera seed...
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Posted by admin on Aug 31, 2015 in |
Density, ρ and viscosity, η of drug of aqueous solution of propylene glycol (PG) (3 m and 7 m) with tert-butanol/2-propanol have been measured over the entire composition range of alkanols at 308.15 K. From this experimental data, excess molar volume, , deviation in viscosity, Δη, and excess Gibbs free energy of activation of viscous flow, ΔG*E have been determined. Positive values of Δη, ΔG*E and negative values of have been observed over the entire composition range in the mixtures studied. The observed positive and negative values of various excess/deviation properties have been attributed to the existence of strong interactions such as geometrical fitting of smaller molecules into the voids created by larger molecules in the liquid mixtures. The excess/deviation properties have been fitted to Redlich–Kister type polynomial and the corresponding standard deviations have been evaluated. The computed partial and excess partial molar volumes data also support the results. The experimental viscosity data of the liquid mixtures investigated have been correlated with viscosity models such as Grunberg and Nissan;...
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Posted by admin on Aug 31, 2015 in |
Sweitenia mahagoni is a natural medicine used to treat various human ailments including diabetes. The present study is a comprehensive approach which targets more than one locus in the diabetic model to enumerate the anti-diabetic potential of Swietenia mahagoni as claimed by folklore. Oral administration of the aqueous extract of the leaf decreased the fasting blood glucose level (155.2±6.7) from its initial value (309.8±10.5) in diabetic rats. There was improvement in the content of innate antioxidant components viz., glutathione (0.570±0.01) when compared to positive control group (0.28±0.02). The activity liver health marker enzymes in the serum were also decreased in the treated group compared to untreated diabetic group. There was also reduction in body mass loss in treated groups. Thus it can be proposed that Sweitenia mahagoni leaf has potent anti-diabetic activity and the effect may be mediated through increasing the antioxidant strength, improving glycogen content in liver, balancing the lipid components in serum, decreasing the muscle protein catabolism and improved overall...
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Posted by admin on Aug 31, 2015 in |
A laxative formulations are widely used for treatment of constipation. They are classified based on their mechanism of action such as bulk forming, stool softener, osmotic, stimulant and mechanical. Bulk forming laxative is most commonly used laxative which works by absorbing water and swelling in small and large intestine to form soft and bulky stool. Presently available bulk forming laxative gives adverse effects such as stomach pain, discomfort and vomiting due to sudden swelling in stomach. Therefore present work was conducted for study of novel bulk forming laxative and minimization of associated adverse effects. Bulk laxative formulation was prepared from watermelon rind, isabgol husk and pectin. Watermelon rind powder (WRP) was prepared and analyzed for proximate composition, carbohydrate content, functional properties and performed for topography by scanning electron microscope. Further, effect of pH on swelling index of WRP was studied at pH 1.3, pH 4.5, pH 6 and pH 7.4. Bulk laxative formulations were developed by varying the concentration of WRP, isabgol and pectin. Developed formulations were evaluated for...
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Posted by admin on Aug 31, 2015 in |
In the present study, bacteriophage based electrochemical analysis is applied to detect pathogenic E.coli bacteria. Phages are parasites that only infect one specific bacteria, can be useful to identify bacterial contaminants in food, water, etc. Phage has been isolated from various water sources such as waste, stagnant and sewage water by overlay method against host bacteria. Titer of phages was calculated to be 107 pfu/ml using 10-fold dilutions. Morphology and identification of phage was done using Transmission Electron Microscopy (TEM) by staining with Uranyl Acetate. The phage capsid was about 78 nanometers (nm) in diameter with tail of length 527 nm as compared to wild type lambda phage whose head is about 65 nm. Plaque reaction activity was observed within 4- 6 hrs against host bacteria by spot test. It is therefore proposed that the isolated phage is lambda like virus and lytic life cycle. Bacteriophages are immobilized onto platinum (pt) electrode by self-assembled molecular monolayer (SAM) and electrochemical impedance analysis performed for a rapid and specific detection of...
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