Posted by admin on Jun 30, 2015 in |
The acaricidal activity of leaf extracts of Annona squamosa, Azadirachta indica and Calotropis procera were tested against the cattle ticks under in vivo condition. Plants were extracted with methanol and water and the extracts were tested against the cattle ticks under in vivo condition. The aqueous and alcoholic extracts of A. indica showed maximum mortality rate of ticks followed by A. squamosa and C. procera when tested individually. In combination of plant extracts, hot water extracts of dried leaf powder showed 100% mortality of ticks on 5th day whereas ethanol and methanol extracts showed 83% and 80% of mortality respectively. Based on the above experimental results, it is confirmed that the selected plant materials possess more acaricidal activity against cattle ticks. Plant extracts in combinations are more effective than single drug...
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Posted by admin on Jun 30, 2015 in |
The present study report deals with the medicinal plants used by the irular tribal people of Coimbatore against pyogenic infections. Five different plants like Abutilon indicum, Datura metel, Lantana Camaro, Tridax procumbens and Leucas aspera were collected and their antimicrobial activity were tested against bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae, Salmonella typhi, Klebsiella pneumonia, Proteus vulgaris and fungi such as Aspergillus niger, Mucor rouxii, Fusarium solani, and Penicillium chrysogenum. All the plants showed antimicrobial activity. Among these Abutilon indicum, Datura metel, Lantana Camaro showed good zone of inhibition against the test organisms. Datura metel showed high zone of inhibition. The extracts of Datura metel showed lowest Minimal inhibitory concentration. Phytochemical analysis showed the presence of alkaloids, flavonoids, saponins and...
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Posted by admin on Jun 30, 2015 in |
The present study a simple, accurate, precise and cost effective UV-Visible spectrophotometric method for development and validation of folic acid as vitamin. folic acid is a water soluble vitamin, so solvent used throughout the experiment was chloroform, butanol,0.1N NaoH, water .the absorption maxima of drug was found at 250nm . Beer’s law was obeyed in the range of 5ppm-30ppm. The developed method was successfully validated with respect to linearity, accuracy and precision. The method was validated and shown linearity in mentioned concentration. The percentage relative standard deviation of inter-day and intra-day precision range 45.15%. Hence proposed method was precise, accurate and cost effective, simple and rapid. This validated method can be applicable for quantitative determination of the titled drug with respect to assay for solid dosage...
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Posted by admin on Jun 30, 2015 in |
Present paper explores the preparation of Nanostructured Lipid Carriers (NLCs) loaded with the anticancer agent docetaxel trihydrate, with potential for targeted anticancer drug delivery. NLCs were prepared using Melt Emulsification Solvent Evaporation – Sonication method. The procedure adopted for preparation was optimized for obtaining NLCs with high entrapment efficiency for the drug and particle size in suitable range, using Quality by Design (QbD) approach employing Design-Expert® software. NLCs were prepared using solid lipids (stearylamine and glycerylmonostearate) and liquid lipids (tocopheryl acetate) with the aid of PhospholiponS100® as emulsifier and LutrolF68® as stabilizer. Independent variables selected were {total lipid to drug ratio} (A) and {solid lipids to liquid lipid ratio} (B). Total lipid to drug ratio (A) was fixed at four levels whereas solid lipid to liquid lipid ratio (B) was fixed at three levels. Optimization batches were prepared in duplicates and entrapment efficiency and mean particle size of each batch was estimated and data analyzed for statistical significance through ANOVA and by response surface and contour plots. The optimized...
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Posted by admin on Jun 30, 2015 in |
The present study was concerned with phytochemical screening of methanolic extract of Datura stramonium seeds and its antioxidant and antimicrobial activities. The methanolic extract was prepared by using Soxhlet apparatus. The result of phytochemical screening of methanolic extract of Datura stramonium seeds shows presence of various phytoconstituents like alkaloids, flavonoids, amino acids, tannins, saponins, carbohydrates and terpenoids. The methanolic extract reduced the concentration of DPPH free radical with an efficacy near to that of standard antioxidant Gallic acid, but less than BHT. The IC50 value for methanolic extract was 0.82mg/ml. The Nitric oxide activity of methanolic extract was also close to the Gallic acid. The IC50 value obtained for the methanolic extract was 0.70mg/ml. The antibacterial activity was detected by agar well diffusion method against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphyloceceus aureus. The zones of inhibitions obtained were recorded and analyzed against standard control of Ampicillin. The methanolic extract showed higher antibacterial activity of 24.0 mm against E.coli and least antibacterial activity of 13mm against P.aeruginosa. The...
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