Posted by admin on Jul 31, 2015 in |
The aim of the current study is to develop a simple, specific, rapid and precise quantification technique for the estimation of tinidazole from tablet dosage form. Successful separation of the drug was carried out on a C18 column (particle size 5 µm, 250 mm length × 4 mm i.d.) using a mobile phase consisting of a 5.3 mM phosphate buffer solution and acetonitrile in the ratio of 60:40 (v/v). The detection wavelength is 318 nm. The method has been validated as per ICH (Q2) guidelines on the basis of accuracy, precession, linearity, sensitivity and robustness. The method is found to be linear with limit of detection and limit of quantitation 0.25μg/ml and 0.76μg/ml respectively. The average elution time is only 5.0 minutes with the analyte elution taking place at about 3.0 minutes making the method rapid and cost effective for routine...
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Posted by admin on Jul 31, 2015 in |
Exhaustive study of scientific literature provides the initial evidence that omega-3-fatty acids the principal component of fish oil is responsible for exerting important biological effects when administered to population at risk of coronary heart diseases and diabetes mellitus. The main reason behind the study was to evaluate the anti-atherogenic properties of marine lipids in alloxan induced diabetic mice. Furthermore the current analysis endeavours to scrutinize the Hypoglycemic effects of the natural omega-3-fatty acids. Mice were made diabetic by a single intraperitoneal (I.P.) injection of alloxan monohydrate 150 mg/kg. Treatment with appropriate dose of omega-3-fatty acid concentrate started 7days after alloxan injection. At the end of the selected period of study the blood glucose level, and lipid profile was evaluated and the data obtained was compared with normal control and positive control. All the data were subjected to due statistical evaluation. The omega-3-fatty acid treatment led to a significant dose dependent decline in the blood glucose content (P<0.05), along with crucial reduction in the lipid profile in alloxan induced diabetic...
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Posted by admin on Jul 31, 2015 in |
The present study was done to evaluate in-vitro membrane stabilizing activity with human RBC, in-vivo neuropharmacological activity including open field and swimming test, gastrointestinal motility and acute toxicity of different leaf extracts of Spondias pinnata in Swiss albino mice following oral administration. In-vivo GI motility test was done by charcoal induced anti motility test, acute toxicity study was done by investigating mortality/morbidity status of test animal and in-vitro membrane stabilizing activity was done with human RBC in both hypotonic and heat induced method. In case of in-vitro membrane stabilizing activity for hypotonic solution induced haemolysis, the ethanol extract inhibited 76.92% haemolysis of RBCs as compared to 85.42% produced by acetyl salicylic acid and during heat induced condition different organic soluble materials of S. pinnata demonstrated 76.68%, 77.19% and 74.83% inhibition of haemolysis of RBCs respectively whereas ASA inhibited 78.04%. In-vivo neuropharmacological activity showed significant (p<0.05) and (p<0.001) results when methanol 200 mg/kg, ethanol 200 mg/kg, chloroform 100 & 200 mg/kg extracts were administered to evaluate the rate of movement...
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Posted by admin on Jul 31, 2015 in |
Background: Sepsis is a significant public health concern and is the main cause of death in surgical intensive care units (ICUs). Patients with sepsis have features consistent with immunosuppression. Taurine is rapidly emerging as one of the more interesting amines which has been reported to have immune-modulatory effect through its action on cytokines. Purpose: To determine the effect of using Immune-enhancing enteral nutritional feed containing taurine compared to Standard enteral nutritional feed on clinical outcomes of ICU septic population. Methods: This was a prospective, randomized, controlled study. A total of 45 patients were randomly divided into 3 groups: Group 1 (n=15) received Standard Enteral Nutrition feed for two weeks, Group 2(n=15) received Immune-Enhancing Enteral Nutrition feed containing 10 mg/kg/day of Taurine for two weeks and Group 3(n=15) received Immune-Enhancing Enteral Nutrition feed containing 30 mg/kg/day of Taurine for two weeks. Parameters measured were serum levels of Interleukin-6, Interleukin-10, C-reactive protein and Total leukocyte count. Results: The current study showed that Taurine had immune-modulatory effect by significantly decreasing the level...
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Posted by admin on Jul 31, 2015 in |
In this study, the possible influence of the model, cationic drug chlorpromazine hydrochloride (CPZ-HCl) concentration on the micro-electrophoretic mobility of neutral large multi-lamellar dimyrstoylphosphatidylcholine (DMPC) liposomes was investigated in various media at both 25oC and 37oC. In addition the effect CPZ-HCl concentration on the micro-electrophoretic mobility of both positively charged DMPC/stearyl amine (ST) liposomes and negatively charged DMPC/dicetyl phosphate (DCP) liposomes were also investigated. The effect of pH on the micro-electrophoretic mobility of neutral positively charged and negatively charged liposomes in the absence and presence of CPZ-HCl in water and in 0.1M KCl at 25oCwere also studied. Lastly, the influence of CPZ-HCl at concentrations known to cause anesthesia, on the micro-electrophoretic mobility of neutral, positively charged and negatively charged liposomes in phosphate buffer (pH 7.4) at 37oC was also investigated. Present study indicates that DMPC liposomes in water exhibited zero electrophoretic mobility over a wide pH range. DMPC liposomes acquired negative charges in 0.1M KCl, phosphate buffer (pH 6.0 and 7.4) and in human plasma (pH 7.4). The addition...
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