Posted by admin on May 30, 2015 in |
This article presents the results of studies on determination of optimal parameters of recombinant strains A/NYMC X-217 (H3N2) and B/NYMC BX-49 of influenza virus cultivation in chicken embryos. It is found that a maximum virus accumulation is formed under the following cultivation conditions: age of chicken embryos is 9-11 days in case of inoculation of the allantoic cavity, inoculated virus dose — 1,000-10,000 EID50, temperature and time of incubation — (34 ± 0.5) ºС and 48 h, respectively. If the cultivation parameters are satisfied, it is possible to obtain a highly active virus-containing material with infectious and hemagglutinating activity, as well as a content of hemagglutinin not less than 7.0lg EID50/ml, 1:256 and 7.0μg/ml, respectively, that is enough to prepare a trivalent inactive seasonal influenza split...
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Posted by admin on May 30, 2015 in |
The antioxidant potential of the aqueous and hydro/ethanolic (ethanol/water) extracts of Crinum jagus were explored considering their total phenolic, flavonoid and proanthocyan contents, 1.1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferrous ions chelating ability and nitric oxide radical inhibiting activity. Hydroethanolic extract has exhibited the highest total polyphenols and flavonoids content as well as the highest DPPH inhibition value (56.71 ± 0.05 % at 320 µg/mL) and ferrous ions chelating ability (60.25 ± 0.22 % at 6 mg/mL), when the aqueous extract showed the highest proanthocyanes content, nitric oxide inhibiting activity comparable to that of ethanol/water extract (respectively 64.52 ± 0.03 % and 65.90 ± 0.43 % with 320 µg/mL extract). The IC50 values of the DPPH and the ferrous ions inhibition were respectively 25.21 µg/mL and 4.69 mg/mL; the IC50 value of nitric oxide inhibiting ability was 16.48 µg/mL and 16.14 µg/mL for the aqueous and hydroethanolic extracts respectively. The results indicate that, Crinum jagus might possess effective in vitro antioxidant activity which could be more marked with ethanol/water...
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Posted by admin on May 30, 2015 in |
Unani and Ayurvedic heath care system is considerable areas of medical treatment in Bangladesh where patient can avail it from different Govt. or private hospital. This cross-sectional study was undertaken to find out the satisfaction of patients attending in this health care system from January to April 2014. A total of 240 respondents were interviewed with an interviewer administered semi-structured questionnaire. Among the respondents majority (54.2%) of them was aged ranging from 21- 40 years. The study revealed that more than 71% respondents were married and majority of them (55.4%) have history of use of Unani/Ayurvedic medicine before. The study found that majority (79.6%) of the respondent has found satisfactory and good quality behavior from the doctor of these systems and it was also found that a good number of respondents (more than 80%) commented doctor’s service as satisfactory and good. It was found that more than 80% respondent got satisfactory and good behavior from pharmacist and concerned stuff of the hospital. Regarding health service delivery system of the...
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Posted by admin on May 30, 2015 in |
The leaves and fruits of Smilax perfoliata are traditionally used for treatment of various ailments such as rheumatism, lumbago, nourishing the functions of spleen, stomach, muscle and bone. The intent of the present study was to evaluate the antioxidant and anti-ulcer activity of ethanolic leaves and fruits extract of Smilax perfoliata Linn in albino rats. Gastric ulcers were induced by employing pylorus ligation method of induction. The anti-ulcer activity was analyzed by estimation of free acidity and the total acidity. The antioxidant potential was analyzed by method of DPPH free radical scavenging activity. The results obtained showed that the leaves and fruits of Smilax perfoliata possess antioxidant potential and are capable in the ministration of gastric...
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Posted by admin on May 30, 2015 in |
In the present study, the hydro-alcoholic and hexane extracts of the plant, Valeriana jatamansi (Family: Valerianaceae) were screened for antimicrobial activity against pathogenic and drug resistant strains. The non polar extracts did not showed any activity against any of the pathogenic strains. Amongst these extracts the hydro-alcoholic extracts of the plant, Valeriana jatamansi, showed potent antimicrobial activity against almost all the pathogens studied. These extracts also showed potent antibacterial activity against multidrug resistant Staphylococcus aureus and Pseudomonas aeruginosa in the ranges from 0.3 mg/ml to 0.7 mg/ml. The potent hydro-alcoholic extract was further subjected for isolation and characterization of potent antimicrobial compound via chromatographic and spectroscopic techniques. The pure compound isolated, Valerenic acid was obtained as colorless liquid with a penetrating aroma; slightly soluble in water, soluble in alcohol, and ether. It melts at -34 0C and boils at 186 0C. The compound showed Rf value 0.37 similar to that of the standard compound, Valerenic acid as detected by TLC. The retention time of the pure compound purified was...
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