Posted by admin on Jul 31, 2015 in |
Diabetes mellitus (DM) was found to impair all dimensions of health except mental health and pain and in a more recent multinational study, DM was found to have a notable impact on general health, measured using the Medical Outcomes Short-Form 36 (SF-36). A cross-sectional, randomized study conducted in Ajman and Sharjah, October 2012 to May 2013, using a self-completed questionnaire and SF-36 health related quality of life (HRQL) questionnaire by 150 diabetic patients on different types of medications and 220 control subjects. Forty four percent of patients used Insulin for their treatment, 34% used Metformin, 14.7% used combination therapy of Insulin with Metformin and only 6% used Gliclazide. The mean values of Physical Functioning (PF), Role-Physical (RP), Bodily Pain (BP), General Health (GH), Vitality (V), Social Functioning (SF), Role Emotional (RE), Mental Health (MH), Physical Components Summary (PCS), and Mental Components Summary (MCS) scores were 61.2, 19.5, 59.2, 56.5, 53.0, 55.4, 57.3, 55.6, 4, and 42.1, respectively. Patients taking an oral therapy had better quality of life than those...
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Posted by admin on Jul 31, 2015 in |
L-asparaginase is responsible for the hydrolysis of L-asparagine into L-aspartic acid and ammonia. L-asparaginase is used as an effective therapeutic agent against lymphocytic leukemia. It also finds applications in food industry. Though much has been unraveled about LA, it may appears today that it is at the tip of iceberg and there is tremendous scope in screening of novel LA sources and studying their properties and applications. In the current study L-asparaginase producing bacteria were screened from forest soil of Vasai, MS, India. Modified M9 medium was used for screening the L-asparaginase producers. Out of the total 885 organisms screened for L-asparaginase production, one isolate was found to produce substantial L-asparaginase (15.94 IU/ml). This isolate was further identified by 16S ribosomal RNA gene sequencing as a novel strain Salinicoccus sp. M KJ997975. This orange pigment producing isolate was found to be moderately halophilic and alkaliphilic. Not much literature is available on L-asparaginase producing gram positive alkalophilic and halophilc bacteria and this is the first kind of report on L-asparaginase...
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Posted by admin on Jul 31, 2015 in |
The contents of Sodium lauryl sulphate (SLS) and Polysorbate (Tween 80) were studied in different formulations of Nebivolol film coated tablets. The contents were extracted using the diluent Methanol: Water in the ratio 60:40 v/v and analyzed in HPLC coupled with Evaporative light scattering detector (ELSD). SLS and Tween 80 are solubilizing agents and the amount of these contents are critical in formulations as they are play a major role in the release pattern of the drug for in-vivo and in-vitro dissolution profiles. A reverse phase liquid chromatographic (RP-LC) method was developed for the quantification of Sodium lauryl sulphate (SLS) and Polysorbate (Tween 80). The method was optimized using buffer (prepared by dissolving 0.77g of Ammonium acetate taken in 2000mL milli-Q-water and then pH was adjusted to 4.5with dilute acetic acid solution) as mobile phase-A and Acetonitrile mobile phase-B. The flow rate was set at 1.0 mL min-1 and the column temperature was maintained at 40°C. The capability of specific method developed was demonstrated by studying the validation parameters...
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Posted by admin on Jul 31, 2015 in |
Present aim to study the biochemical composition, i.e., protein, carbohydrate, lipid, ash, amino acids, fatty acids, vitamins, minerals and Carbon, Hydrogen, Nitrogen from Skeletonema costatum. The biomass of marine diatom (S.costatum) were collected from Parangipettai, Southeast cost of India. The lyophilized powder of S.costatum was used for biochemical analysis. The results of proximate composition showed that the percentage of protein was (12.12%), (carbohydrate 5.94%), (lipid 1.567%) and ash (2.96%). Totally 10 essential and non-essential amino acids were analyzed (24.49mg/g – 22.36 mg/g). The saturated mono unsaturated and poly unsaturated fatty acids was (14.86%), (4.25%) and (10.86%). The vitamins and mineral content was 384.19 mg/g – 459.64 mg/g respectively and 1.627 microgram dry powder of Skeletonema costatum contained the ratio of Carbon (15.04%), Hydrogen (2.59%) and Nitrogen (3.17%) were present. The results of this research showed that S.costatum contained valuable nutritional values and we suggest that in future research on S.costatum can be use food for...
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Posted by admin on Jul 31, 2015 in |
The aim of present work was to develop and evaluate solid lipid nanoparticles (SLNs) based gel for topical delivery of antifungal agent. Bifonazole (BFZ) is an imidazole antifungal agent having broad spectrum activity against dermatophytes, moulds, yeasts, fungi and some gram positive bacteria. BFZ SLNs systems were developed by melt emulsification followed by ultrasonication technique using Precirol ATO5 as a solid lipid and Tween 80 as a surfactant. Developed SLNs were evaluated for particle size, polydispersity index (PI), entrapment efficiency (EE) and drug release profiles. Process and formulation parameters were optimized. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) studies were carried out on SLNs to mark the changes in the drug and lipid modifications. The BFZ SLNs based gels were prepared using Carbopol 940 as a gelling agent. The SLNs based gels were evaluated for rheological parameters, in vitro drug release and permeation studies. In vitro antifungal study suggested that the SLNs based gel was more effective in inhibiting growth of Candida albicans. Thus the study concludes that...
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