Posted by admin on Jul 31, 2015 in |
The present study is to investigate the cytotoxicity properties and haematological indices of synthesized molecules (S1-S4) in comparison to the standard drug 5 fluorouracil which reflects the anticancer potentials of the above molecules. Cytotoxicity studies were performed by in vitro MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl teterazolium bromide) assay. Haematological studies were done by drawing blood in the drug and synthesized molecules administered animals by retroorbital method. Haemoglobin, RBC, WBC, lymphocytes, Monocytes and Granulocytes counts were measured. All the above experiments were performed against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. The percentage cytotoxicity and percentage viable cell counts revealed anticancer properties of the synthesized molecules. RBC count indicated protection of cell membrane from destruction and the WBC counts along with lymphocytes, Monocytes and granulocytes also show the immunoprotective characteristics of the novel molecules. Thus it has been concluded that the synthesized molecules (S1-S4) if supported by further molecular studies may have a promising role to play as anticancer...
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Posted by admin on Jul 31, 2015 in |
Medicinal plants have been investigated for possible anti-cancer effects. Corallocarpus epigaeus (Roth & willd.) cl. is an ethnobotanically important wild climber that belongs to the family Cucurbitaceae. The aim of the present study is to examine the cytotoxic activity of Corallocarpus epigaeus (Roth & willd.) cl., on two tumor cell lines. The methanolic root extract of the plant Corallocarpus epigaeus (Roth & willd.) cl. was subjected to cytotoxic activity study against two human cancer cell lines (MCF7, human breast adenocarcinoma and HT-29, human colon carcinoma) using the MTT assay. The concentration of methanolic root extract yields the IC50 value of 183.33±0.7 µg/ml and <62.5µg/ml respectively for HT-29 and MCF-7 cell lines by MTT assay. The results showed that the root extract of Corallocarpus epigaeus has potential anti-cancer activity against MCF-7 and HT-29 human cancer cell...
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Posted by admin on Jul 31, 2015 in |
Citrullus vulgaris Schrad. has occupied a popular place in the indigenous system of folk medicine because of its high nutritional and antioxidant properties. The study aims to elucidate the extractive yield percentage and screen the seed extracts of watermelon using different solvents for its phytochemical profile. The solvents selected were Methanol, Ethanol, Chloroform, Petroleum ether, Ethyl acetate, Acetone and Hot aqueous extracts. The shade dried powdered seed material was subjected to Soxhlet extraction using different solvents and Pressurized hot water extraction. The resultant extracts were concentrated and the preliminary screening for phytochemical profiling was carried out using standard laboratory protocols. Analysis of the extractive yield percentages using different solvent extracts and pressurized hot water extract showed hot aqueous seed extract to have the highest yield percentage. The seed extracts with different solvents and pressurized hot water extract were screened and tabulated with the presence and/or absence of various phytochemical constituents such as the primary metabolites and the secondary metabolites and various other bioactive elements which are thought to be...
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Posted by admin on Jul 31, 2015 in |
A reverse phase liquid chromatography method (RP-HPLC) was developed to estimate the amount of Indapmide and Perindopril in bulk and its pharmaceutical formulations. A Waters Alliance HPLC system equipped with auto sampler, UV-Visible detector and YMC Column (150 x 4.6mm, 3µ particle size) were used for the quantification of the drugs. Separation was carried out by using potassium dihydrogen phosphate buffer of pH 2.5 and acetonitrile in the ratio 60:40 v/v as mobile phase at a flow rate of 1 ml/min and the detection was carried out at a wavelength of 230 nm. The retention time, tailing factor and USP theoretical plates were found to be 2.5 min., 1.38 and 2551 for Indapamide and 4.18., 1.75 and 2778 for Perindopril respectively. The area of the peak was proportional to the concentration of the drug in the range 15-35, 48-112 μg/ml of Indapmide and Perindopril. The values of LOD and LOQ for Indapmide and Perindopril were found to be 0.07, 27.87 and 0.23, 84.47 μg/ml respectively. The mean recoveries of...
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Posted by admin on Jul 31, 2015 in |
In the present investigation, controlled release microparticles of Glimepiride were prepared by emulsion solvent evaporation technique using cellulose acetate as release retardant polymer. The microparticles were evaluated for In-Vitro release study and In-Vivo evaluation. The microparticles were subjected to In-vitro release studies by employing 7.8 pH phosphate buffer as dissolution medium. The rate of drug release from these microparticles followed zero order kinetics and the mechanism of drug release was governed by peppas mechanism. The exponential coefficient (n) value indicating that drug release followed non fickian mechanism. The in vivo performance of pure drug and optimized Glimepiride microparticles were evaluated in rabbits in a randomized cross-over design. The estimation of glimepiride in serum was carried out by HPLC method. The parameters such as maximum plasma concentration (Cmax), time for peak plasma concentration (tmax), mean residence time (MRT) and area under curve (AUC0 – α) were significantly (P< 0.001) differed following glimepiride microparticles compared to pure drug administration. The relative bioavailability of glimepiride was increased about five fold after microparticles...
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