Posted by admin on May 30, 2015 in |
The skin penetration and safety of nanometric zinc oxide and titanium dioxide incorporated in a cosmeceutical cream, specifically designed to retard skin actinic damage in tropical Oculocutaneous albinism was investigated ex-vivo through simulated actinically damaged porcine skin. Franz diffusion cells were used in an experiment modeled in line with related work done by Diembeck et al (1999); the OECD guideline, document no 428 and the EU opinion SCCNFP 0750/03. The following experiment was distinguished by its use of simulated actinic damaged skin characteristic in albinism in the tropics. The subsequent analytical studies were modified, since skin stripping was not practical on damaged skin. Analysis for the nanomaterials was conducted on both the Franz cell receptor phase fluid as well as extractions from the entire skin tissue material after the diffusion process. Quantification analysis for the recovered titanium was done by ICP-AES and the zinc was assayed using Flame AAS. The total recoveries of zinc from the skin extracts ranged between101.35-103.20% of the total zinc applied. The amounts of zinc...
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Posted by admin on May 30, 2015 in |
Introduction: Angiogenesis is the formation of new blood vessels which is a process involved in development of tumors and other neovascular diseases. Developmental angiogenesis in zebra fish embryos is a relevant and emergent model to evaluate the anti-angiogenesis activity which was used in present study. With this model, blood vessel formation and the blood flow can be clearly observed. Various parts of Neem tree were proved to have potential anticancer activity. Based on this fact, neem root has been selected for this study. Method: Zebra fish embryos were treated with different concentrations of Water soluble fractions of crude methanolic extract of neem root, Imatinib (standard) and control. Then, they were observed for 3 days within 24 hour interval. Results: Various phenotypical abnormalities that were observed during the development of larvae had been captured using digital microscope. Toxicity and survival rates were noted. Conclusion: Based on the results obtained, the water soluble fractions of methanolic extract of neem root was found to have the ability to inhibit in -vivo angiogenesis....
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Posted by admin on May 30, 2015 in |
Training under hypoxic conditions is known to enhance physical performance. The possible mechanism might be the stabilization of Hypoxia Inducible Factor (HIF), which otherwise is degraded under normoxia by the action of Prolyl Hydroxylase domain enzymes (PHD). Stabilization of HIF leads to upregulation of its target genes leading to improved erythropoiesis, angiogenesis and antioxidant status as an adaptation to combat training induced stress. Present study investigates efficacy of Ethyl 3, 4 Dihydroxybenzoate (EDHB) in stabilizing HIF-1α which leads to elevated levels of Vascular Endothelial Growth Factor, Erythropoetin, Hb and Hct, indicating improved angiogenesis and blood oxygen carrying capacity. In the present study we have reported 1.5 times increase in endurance performance in rats supplemented with EDHB with or without training as compared to respective controls. Significant improvement in antioxidant status as observed by increase in GSH, SOD, GST and decrease in MDA and protein oxidation, enhanced expression of anti-oxidative proteins Heme-Oxygenase, Metallothionein, Nuclear Factor Erythroid 2-Related Factor contributed in decreasing exercise training induced oxidative stress in EDHB supplemented trained...
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Posted by admin on May 30, 2015 in |
The effect of amphiphilic polyanion Poly(maleic acid-alt-octadecene)sodium salt on solubility of a zwitterionic drug (ampicillin trihydrate) in aqueous media was evaluated. This polymeric salt was obtained by an equimolar reaction of parent copolymer poly Poly(maleic acid-alt-octadecene)with an aqueous solution of NaOH. The obtained amphiphilic polyanion was purified by dialysis and lyophilization and was characterized by FTIR spectroscopy. Secondly, a method for the model drug quantification by UV spectrophotometry at 37°C was standardized. Also the grade of association drug-polymer in aqueous media using ultrafiltration methodology at three levels of temperature (20, 30 and 40°C) and two levels of pH (1.2 and 7.4) was determined. Finally, the variation of aqueous solubility of ampicillin trihydrate in ultra-pure water and two buffer media with pH of 1.2 and 7.4 with an ionic strength of 0.15 M at 37°C in presence and absence of amphiphilic polyanion was evaluated. It was found that maximum solubility is reached at a pH value of 1.2 in absence of polymeric material with a value of 36 mg/mL, which...
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Posted by admin on May 30, 2015 in |
The pharmacokinetic/pharmacodynamic indices generally predict the efficacy of antibiotics when used alone. The information related to these indices for the combination products and that too in cases of resistant bacterial infections is limited. The present study involved treatment of 12 subjects with severe complicated urinary tract infections caused by resistant extended spectrum beta lactamase bacterial strains with Elores; ceftriaxone and sulbactam combination with non-antibiotic adjuvant ethylenediamine tetracetate. In this study Elores 3g bd dose was administered and the pharmacokinetic/pharmacodynamic indices were evaluated. To see the impact of lower Elores dose on these indices, 3g od data was simulated using pharmacokinetic parameters from 3g bd dose. The pharmacokinetic/pharmacodynamic indices suggest that Elores 3g od can treat mild to moderate infection wherein minimum inhibitory concentration requirement is <16µg/mL; however for severe complicated urinary tract infections with resistant strains wherein the requirement is 16‑32µg/mL, Elores 3g bd should be the...
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