Posted by admin on Apr 30, 2015 in |
Topical NSAIDs do not effectively cross the skin due to barrier of stratum corneum. The aim of the study was to evaluate penetration of diclofenac from a novel quick penetrating solution (QPS) and conventional gel prepared using Tc-99 m labeled diclofenac. The study was conducted in 16 healthy subjects, divided in 4 groups, each with 4 subjects. In first three groups, the applications were made on knees for 30, 60 and 120 minutes respectively. In fourth group, applications were made at six sites (three on either side of the spine) on the back. Equal quantity of diclofenac from QPS and gel were applied on left and right knee and left and right side of the spine respectively. Measurement of radioactivity was done by gamma camera after washing of the applied drug. It was found that the penetration of radiolabeled diclofenac increased with increase in application time for both the QPS and gel formulations. Scintigraphy images indicated site specific differences in the rate and extent of penetration. Over knee, the...
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Posted by admin on Apr 30, 2015 in |
A simple, specific, and precise stability indicating reverse phase high performance liquid chromatography method was developed and validated as per the ICH guidelines for the simultaneous determination of Moxifloxacin and Prednisolone in bulk and combined dosage forms. The quantification was carried out by using Zodiac C18 (250mm*4.6mm, 5μ) column at 250c with Acetate Buffer pH 4.5: Methanol: Acetonitrile in ratio of 50:20:30 % V/V as mobile phase. The flow rate is 1 mL/min and the estimation was carried out by using PDA detector at 271 nm. The retention time of MFX and PDS were 2.317 and 4.310 minutes respectively. The linearity was observed from 30-70μg/mL with correlation coefficient 0.9999 for Moxifloxacin and 60-140 μg/mL with correlation coefficient 0.9998 for Prednisolone. The LOD and LOQ of Moxifloxacin and Prednisolone were found to be 4.85 & 14.69μg/mL and 9.07 and 27.47μg/mL respectively and the Statistics data for the MFX and PDS were concluded that the method was found to be simple, reliable, selective, reproducible and accurate. The method was successfully used...
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Posted by admin on Apr 30, 2015 in |
Nano-emulsions consist of fine oil-in-water or water-in-oil dispersions, having droplets covering the size range of 10 – 600 nm. The aim of this study is to formulate nano-emulsion of Clindamycin by using Emulsion Phase Inversion method and olive oil as the oil phase. Pseudo ternary phase diagram was first developed by using distilled water, olive oil and mixture of surfactants (Tween®80 and Span®20) at a ratio of 1:1. Then, appearance test and microscopic examination were done for all the pre-formulation. Three potential pre-formulation were then selected and incorporated with the Clindamycin Phosphate and Methyl Paraben. The mean droplet size and stability studies were done for these three formulations. Clindamycin Nano-emulsions were not obtained using the Emulsion Phase Inversion (EPI) method in this study, whereby the mean droplet sizes were in micro-range. However, out of all the three formulations which undergone extensive studies which include the heating-cooling cycle, whereby the formulation F8 and F17 were found to be physically stable. Significant differences were identified on the pH value and viscosity...
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Posted by admin on Apr 30, 2015 in |
Mallasindura (MSL), a widely used ayurvedic preparation is used for the treatment of bronchial asthma. It contains rasa –mercury, Rasakarpura -mercurial compound, Bali –purified and processed sulphur and Malla-purified arsenic oxide with herbal juices. The liver function parameters of rat plasma were studied after chronic administration of MSL. The animals used to conduct this experiment were albino rats (Rattusnovergicus: Sprague-Dawley strains) and the drug was administered per oral route. The administered dose of the drug was 100mg/kg body weight, once daily up to 90 days for the whole study. There were forty rats for four randomized groups, equally of both sexes. Among them one male and one female group were used as test. In case of male rats, there was increase in total protein and albumin content, which was statistically highly significant with a p value of 0.001. Along with this, a statistically highly significant declining trend was followed for bilirubin, sGPT, sGOT activities (p values are 0.001 and 0.009 respectively). In case of female rats, a statistically highly...
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Posted by admin on Apr 30, 2015 in |
Ficus dalhousiae Miq. is a plant found in Andhra Pradesh and Tamil Nadu on rocky hill top of dry deciduous forests. As it possesses antioxidant property wherein it reduces the elevated levels of ROS, the intent of the present study was to evaluate the Gastro protective effect of Ficus dalhousiae Miq Root ethanolic extract (FDREE) by means of Indomethacin and Cold Restrain stress induced Ulcers in Albino rats. Indomethacin 5mg/kg body weight p.o; for five days and Cold restraint stress models, were used for inducing gastric ulcers in rats. Biochemical parameters such as Glutathione, Malondialdehyde, acidity, Gastric volume, gastric pH and Ulcer index were determined in order to assess the gastro protective activity of FDREE in both the models. Treatment of rats with Indomethacin and subjecting them to Cold restraint stress (CRS) elevated the levels of Gastric volume, acidity, Glutathione, Malondialdehyde and gastric pH in negative control group in comparison with normal group. The elevated levels were significantly reversed when treated with standard drug Ranitidine 50 mg/kg body weight...
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