Posted by admin on Apr 30, 2015 in |
A simple, rapid and sensitive liquid chromatography-tandem mass spectrometric (LC–MS/ MS) assay method has been developed and fully validated for the quantification of Teriflunomide (TFM), in different biological matrices like human plasma, rat plasma, rabbit plasma and dog plasma and as well as their serums. The analytes were extracted from 200 µL aliquots of each plasma/serum via protein precipitation using acetonitrile. The extracted samples were chromatographed on a XBridge C18 column by using acetonitrile and 5 mM ammonium formate (pH 9.0) as the mobile phase in gradient mode at a flow rate of 1.0 mL/min. The calibration curves obtained were linear (r2 ≥ 0.98) over the concentration range of 1.0–4000 ng/mL. Detection was carried out on a triple quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) with an electro spray ionization (ESI) mode using the respective m/z 268.8→82 for TFM and 281→86 for IS. The method was validated interms of specificity, accuracy, precision and limit of quantification. The results of the intra-and inter-day precision and accuracy studies were...
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Posted by admin on Apr 30, 2015 in |
The discovery of the f-channel and its role in regulating pacemaker activity lead to the development of new pharmacological agents such as ivabradine, which target these f-channels causing a reduction in heart rate by inhibiting the /fcurrent. The aim of the present work was designed to evaluate the cardioprotective effect of ivabradine on experimentally- induced myocardial infarction and adrenaline-induced arrhythmia in rats. In addition, the present work studied the effect of ivabradine on isolated rabbit’s aortic spiral strip and isolated rabbit’s heart. Acute myocardial infarction in rats was induced by isoperameline (150mg/kg subcutenous injection, once) 24 rats were divided into the following groups: group (1) control normal rats, group (II) myocardial infarction – induced rats with no previous treatment, group (III) myocardial infarction – induced rats pretreated with ivabradine (10mg/kg/day) for one week and group (IV)) myocardial infarction – induced rats pretreated with carvedilol (1mg/kg/day) for one week. Electrophysiological, biochemical and histopathological parameters were estimated. pretreatment with either ivabradine or carvedilol show significant improvement in all these parameters with...
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Posted by admin on Apr 30, 2015 in |
The reaction between 2-naphthol, aryl aldehydes and ammonia or amines yields aminobenzyl naphthols in process known as Betti reaction; this procedure can be interpreted as extension of the Mannich condensation, with formaldehyde replaced by aromatic aldehydes, secondary amine by ammonia and the C – H acid by an electron-rich aromatic compound such as 2-naphthol. 1,3-Disubstituted-2,3-dihydro-1H-naphth[1,2-e][1,3]oxazines were prepared through reactions of 2-naphthol, aromatic aldehydes and amines in ratio 1:2:1 in presence of methanol. Acid hydrolysis of 1,3-naphthoxazines using 20% HCl produce derivatives of Betti bases, Betti bases were prepared directly via the reaction between 2–naphthol, benzaldehyde and ammonia solution or aliphatic amines (primary and secondary) in ratio 1:1:1 in presence of water. Some of the Betti base derivatives which were synthesized by the two methods reacted with diazonium salts and produce colour compounds in moderate to high yield known as azo dyes. The structures of the all synthesized compounds were confirmed by IR, H1-NMR, and MS spectral studies. Results of this study revealed that, the Betti base derivatives which were...
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Posted by admin on Apr 30, 2015 in |
Rosella (Hibiscus sabdariffa L) calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP) to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM) and particle size analyzer (PSA). We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan...
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Posted by admin on Apr 30, 2015 in |
Over production of oxygen free radicals, which is mainly mediated by super oxide, occurs in human atherosclerosis. Metoprolol and atorvastatin are the drugs which found to be more prescribed drugs for cardiovascular patients. In the present study, an attempt has been made to evaluate the levels of serum Glutathione, Total anti-oxidant status, lipid peroxidation (malondialdehide) and liver function parameters like serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase. Total 68 subjects were selected for study. Out of 68 subjects 26 were normal healthy volunteers and 42 subjects were patients who are untreated with cardiovascular diseases. The patients both male and female within the age group of 20-70 years were selected. Glutathione, total anti oxidant status, lipid peroxidation, serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase levels in plasma were estimated by using reported methods. Individual methods were standardized first and standard graphs were plotted and the parameters were measured significantly decreased values of glutathione, total anti oxidant status and malondialdehide were observed (p<0.001) in untreated cardiovascular patients as compared with normal...
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