Posted by admin on Apr 30, 2015 in |
We have developed a novel LAMP-based molecular marker for easy and fast identification of Podophyllum hexandrum Royle (Indian May apple). It is an important medicinal plant valued all over the world. It have both anti-cancer (pro-oxidant ability) and radioprotective (antioxidant ability) effects respectively. The LAMP-based molecular was constructed based on genomic DNA of P. hexandrum that amplified by RAPD. A RAPD amplicon common to all accessions, was eluted, cloned and sequenced. Based on these sequences in-house specific LAMP primers were synthesized. This method employs by a Bst DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the P. hexandrum genomic DNA. In LAMP reaction the amplification was carried out in thermal cycler and water bath at 60°C for 60 min, which was followed by monitoring DNA amplification with the naked eye through color changes by standardizing SyBr Green® I nucleic acid stain. The data showed confirmatory results in both thermal cycler and water bath. Since, the assay method is...
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Posted by admin on Apr 30, 2015 in |
To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabean, the present study was intended to evaluate the in-vitro immunomodulatory activity of dry seeds of Glycine max. L. Effect of ethanolic and methanolic extracts of soyabean was evaluated at various concentrations (800μg/ml to 6.25μg/ml) for secretion of various mediators. Immunomodulatory activity of both extracts was examined on murine macrophage phagocytosis by Nitroblue tetrazolium (NBT) dye reduction, nitric oxide, lysosomal enzyme activity and myeloperoxidase activity. Proliferation of spleenocytes and bone marrow cells was evaluated by 3 (4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) as mitogen. Both aqueous and acetone extracts presented immunomodulatory activity in peritoneal mouse macrophages, bone marrow cells and spleenocytes without clear dose response relationship. In-vitro phagocytic index and proliferation index showed significant results and thus proving the need for confirmation through in-vivo...
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Posted by admin on Apr 30, 2015 in |
Diabetes mellitus is an endocrine disorder, in which a person has high blood sugar, and the body does not produce sufficient insulin, or cells do not act in response to the insulin that is produced. Diabetes mellitus is a complicated metabolic disease that has gravely affected the human health and quality of life. As increase in carbohydrate intake, especially of fructose and high rich diet are both factors that contribute to the development of diabetes mellitus. In the present study, the characterization of fructose diet induced diabetes mellitus in Swiss albino mice was studied. Animals were divided into two groups, group A (Control) and group B (Fed with fructose diet). Oral administration of 10% fructose solution to Swiss albino mice for 10 weeks resulted in diabetes mellitus. For the duration of the experiment bodyweight, fasting blood glucose level and biochemical parameters (total cholesterol, total serum protein, serum creatinine and blood urea) were measured. The study revealed that fructose diet resulted in significant increase in bodyweight, fasting blood glucose level,...
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Posted by admin on Apr 30, 2015 in |
Advancement in mobile technologies are seen with introduction of many smartphone applications. These advancements in the field of smartphone applications can be exploited to improve pharmaceutical care practice ultimately leading to betterment of patients. Objectives of the study were to identify, evaluate and review current smartphone applications available at various platforms and recommended for pharmaceutical care. Data was extracted from platforms Android’s Google play store, Nokia’s Ovi store, Windows and marketplace for smartphone applications pertinent to pharmaceutical care. From all the applications accessed through various platforms the five stars rated freely downloadable smart phone applications were selected, tabulated and further evaluated by using standard questionnaire. In this study a total of 194 apps were identified in android, windows and ovi store mobile platforms of which 99 applications were from Android’s Google Play Store, 48 applications from Windows store and 47 applications were available in Ovi stores. 74% of them were free to download and 132 of 194 apps i.e. 68% had customer satisfaction ratings and only nine apps had...
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Posted by admin on Apr 30, 2015 in |
Topical NSAIDs do not effectively cross the skin due to barrier of stratum corneum. The aim of the study was to evaluate penetration of diclofenac from a novel quick penetrating solution (QPS) and conventional gel prepared using Tc-99 m labeled diclofenac. The study was conducted in 16 healthy subjects, divided in 4 groups, each with 4 subjects. In first three groups, the applications were made on knees for 30, 60 and 120 minutes respectively. In fourth group, applications were made at six sites (three on either side of the spine) on the back. Equal quantity of diclofenac from QPS and gel were applied on left and right knee and left and right side of the spine respectively. Measurement of radioactivity was done by gamma camera after washing of the applied drug. It was found that the penetration of radiolabeled diclofenac increased with increase in application time for both the QPS and gel formulations. Scintigraphy images indicated site specific differences in the rate and extent of penetration. Over knee, the...
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