Posted by admin on Mar 30, 2015 in |
The aim of the present study was to isolate, identify and to analyze the phylogenetic characteristics of the potent actinobacterial strain VL-RK_05 isolated from Mango orchards of Vissannapet, Krishna Dist, Andhra Pradesh. Soil samples collected were pre treated with calcium carbonate and used for isolation of potent Actinobacterial strain designated as VL-RK_05. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S r RNA sequence based analysis. Phylogenetic tree was constructed using the Molecular Evolutionary Genetic analysis version 5. Phylogenetic analysis of 16S r RNA sequencing revealed that the strain is closely related to Rhodococcus erythropolis and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Shigella flexneri, Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida...
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Posted by admin on Mar 30, 2015 in |
Leaves and bark preparations of Ficus dalhousiae Miq (Moraceae) are used in folklore and Siddha system of medicine for liver and skin ailments. So the intent of the present study was to evaluate the Hepatoprotective effect of Ficus dalhousiae Miq leaves ethanolic extract (FDLEE) by means of Carbon tetrachloride and Paracetamol induced hepatotoxicity model in Albino rats. Administration of carbon tetrachloride suspended in liquid paraffin 1ml/kg body weight i.p, and Paracetamol in distilled water (2 gm/kg) body weight; p.o. to albino Wistar rats resulted in hepatotoxicity. Biochemical parameters [Alkaline Transaminase (ALT/SGPT), Aspartate Transaminase (AST/SGOT), alkaline phosphatase (ALP), Total Bilirubin (TB), Direct Bilirubin (DB), Albumin (ALB), Total Protein (TP)] and liver weight were measured to determine the degree of protection at the end of duration of treatment in both the models. CCl4 and Paracetamol markedly elevated the levels of SGPT, SGOT, ALP, TB and DB. Treatment with the standard drug Silymarin (25mg/kg) reversed all the parameters and restored them to the optimum levels. The test extract FDLEE produced a significant...
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Posted by admin on Mar 30, 2015 in |
The aim of the present study is an attempt to evaluate the antimicrobial activity of the methanolic extract of the ink of cuttlefish, Sepia pharaonis on bacterial pathogens. The methanolic extract of the ink of cuttlefish, Sepia pharaonis were prepared and the antibacterial activity was determined by agar well diffusion method on different bacterial strains (Bacillus sp., Pseudomonas sp., E coli., Staphylococcus sp. and Klebsiella sp.). Isolation and the purification of the antibacterial principle were carried out by using Thin Layer Chromatography (TLC). The thermal stability of the extract was determined by incubating the extract at different temperatures. The methanolic extract of the ink of cuttlefish showed antibacterial activity on different bacterial strains. Of the five different species used, the bacterial species Bacillus were found to be more sensitive than all the other species. The bacterial strains Pseudomonas sp., Staphylococcus sp. and Klebsiella sp. were found to be moderately sensitive to the methanolic extract of the ink of cuttlefish. The species E coli is found to be least sensitive...
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Posted by admin on Mar 30, 2015 in |
Simple, sensitive, precise, accurate, and specific reversed –phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of levofloxacinin pharmaceutical tablets. Levofloxacin is a third generation fluoroquinolones with a wide spectrum of action against gram-positive and gram-negative bacteria, anaerobic microorganisms and atypical pathogens. It is pure (-)-(S)-enantiomer of the racemic drug substance ofloxacin, which was introduced in 1997. Isocratic chromatography was performed on a C18 column with acetonitrile‐methanol‐phosphate buffer 0.1M15:25:60 (v/v/v) as mobile phase at a flow rate of 1 ml/min. UV detection was set at 287 nm. The fluorescence detector was set at excitation/emission wavelengths of 300/500 nm. The method was validated with respect to accuracy, linearity, precision, and selectivity. All the parameters examined met the current recommendations of U.S.P (30) for analytical method validation. The method can be reliably used for routine quality control analysis and to determine the levofloxacin content of marketed...
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Posted by admin on Mar 30, 2015 in |
Main aim of this study was to find out relation of CYP2D6 polymorphism with tramadol action in post herpetic neuralgia (PHN) patients. This non- responders versus responders study mainly includes 246 patients (age group 20-80 years) of PHN, assigned into two groups depending upon their response to tramadol treatment at the end of 2nd week of drug therapy. On the basis of CYP2D6 polymorphism, an allodynia and pins and needles significant interactions were found with CYP2D6*2 allele between time, group and metabolizers while with*4 and *10 alleles no interaction observed. Finally, results not showing role of CYP2D6 polymorphism in the development of neuropathic symptoms in PHN patients...
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