Posted by admin on May 30, 2015 in |
India having a large stretch of marine coastline is advantageous as a natural resource for marine aquaculture. But the waste produce from the marine processing industries fetch a greater environmental risk as there is accumulation of wastes in huge quantity. These wastes are a larger source of natural carotenoids that have a huge pharmaceutical potentials. So proper initiatives must be taken to recycle the waste into a product. Natural astaxanthin from crustacean shell waste is a better alternative to the synthetic orange-red pigment. In this present investigation astaxanthin was extracted from crustacean shell waste, by using organic solvents of different polarities (Hexane, methanol, ethanol, petroleum ether, chloroform and acetone). Characterization of the astaxanthin pigment were performed by TLC buy comparing the Retardation factor (Rf) as indicated in Lorenz Todd standard chromatogram where three bands of Astaxanthin (Rf=0.36), Astaxanthin monoester(Rf=0.60) and Astaxanthin diester (Rf=0.75) were detected. The extraction yield of the natural carotenoid from shrimp waste was compared amongst all solvents spectrophotometrically at 470 nm and quantified using equation of...
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Posted by admin on May 30, 2015 in |
The global rise in dengue cases is a major public health concern in terms of morbidity and mortality. The recent study reports 390 million dengue infections annually of which 96 million infections becomes clinically or subclinically severe. Therefore, development of an effective tetravalent vaccine against dengue is a top priority. Dengue envelope domain III is a surface exposed protein; involved in host cell binding and containing multiple, serotype-specific and subcomplex-specific neutralizing epitopes, thus becomes an ideal target for vaccine development. The rapid growth in bioinformatics or immunoinformatics area in terms of development of sophisticated tools assists researchers to predict immunodominant epitopes and study various characteristics of the predicted vaccine model. The combination of computer-aided or in silico methods and experimental methods are useful tools to address complex problems such as deciphering immune responses and vaccine design. In the present study we aim to develop a recombinant tetravalent vaccine model using bioinformatics tools of our vaccine candidate containing envelope domain III of all four dengue serotypes (GenBank ID: KF 855114)...
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Posted by admin on May 30, 2015 in |
Biologically potent compounds are one of the most important classes of materials for the upcoming generations. Increasing number of microbial infectious diseases and resistant pathogens create a demand and urgency to develop novel, potent, safe and improved variety of antimicrobial agents. This initiates a task for current chemistry to synthesize compounds that show promising activity as therapeutic agents with lower toxicity. Therefore, a substantial research is needed for their discovery and improvement. Transition metal complexes share an important place in this regards. Further, it is evidenced that complexation of above metal ions with nitrogen and sulphur donor ligands increases the efficiency of biocidal activity. For the same, metallic soaps of copper (derived from common fatty acids) were complexed with substituted 2-amino benzothiazole derivatives. Complexes were characterized by elemental analysis IR, NMR and ESR spectral data. Their purity was checked by Thin Layer Chromatography. Their antimicrobial efficacy / biological activity was examined against selective bacteria such as Pseudomonas aeruginosa (Gram negative), Lactobacillus aciedophillus (Gram positive), antifungal activity against Fusarium semitectum...
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Posted by admin on May 30, 2015 in |
The skin penetration and safety of nanometric zinc oxide and titanium dioxide incorporated in a cosmeceutical cream, specifically designed to retard skin actinic damage in tropical Oculocutaneous albinism was investigated ex-vivo through simulated actinically damaged porcine skin. Franz diffusion cells were used in an experiment modeled in line with related work done by Diembeck et al (1999); the OECD guideline, document no 428 and the EU opinion SCCNFP 0750/03. The following experiment was distinguished by its use of simulated actinic damaged skin characteristic in albinism in the tropics. The subsequent analytical studies were modified, since skin stripping was not practical on damaged skin. Analysis for the nanomaterials was conducted on both the Franz cell receptor phase fluid as well as extractions from the entire skin tissue material after the diffusion process. Quantification analysis for the recovered titanium was done by ICP-AES and the zinc was assayed using Flame AAS. The total recoveries of zinc from the skin extracts ranged between101.35-103.20% of the total zinc applied. The amounts of zinc...
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Posted by admin on May 30, 2015 in |
Introduction: Angiogenesis is the formation of new blood vessels which is a process involved in development of tumors and other neovascular diseases. Developmental angiogenesis in zebra fish embryos is a relevant and emergent model to evaluate the anti-angiogenesis activity which was used in present study. With this model, blood vessel formation and the blood flow can be clearly observed. Various parts of Neem tree were proved to have potential anticancer activity. Based on this fact, neem root has been selected for this study. Method: Zebra fish embryos were treated with different concentrations of Water soluble fractions of crude methanolic extract of neem root, Imatinib (standard) and control. Then, they were observed for 3 days within 24 hour interval. Results: Various phenotypical abnormalities that were observed during the development of larvae had been captured using digital microscope. Toxicity and survival rates were noted. Conclusion: Based on the results obtained, the water soluble fractions of methanolic extract of neem root was found to have the ability to inhibit in -vivo angiogenesis....
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