Posted by admin on Jul 31, 2015 in |
The present study aimed to evaluate the antitumor efficacy of Snake venom (SV), bee venom (BV), combinations of their components and their apoptotic, cell death mechanisms in liver (HepG2) and breast (MCF7) cell lines. Cytotoxic effects of venoms, L-Amino acid oxidase (svLAAO), phospholipase A2(svPLA2), Melittin ( MEL) were tested against HepG2 and MCF-7 cell lines and IC50 was calculated . mRNA expression of p53, Bax and Bcl-2 was carried out. All the tested compounds had anti proliferative effects on the tumor cell lines with different potency. BV had a higher cytotoxicity against liver and breast cells (IC50=1.26 and 2.85 µg/ml) than SV (IC50=5.86, 13.05µg /ml). The cytotoxicity of svLAAO was much higher than SV with IC50 = 3.65 and 0.48 µg /ml. MEL showed a higher cytotoxic effect than BV against MCF7 cells (IC50=1.14 µg /ml). MEL when combined with svPLA2 gave a synergistic effect on the expression of P53 and Bax in the two cancer cells. Venoms combination had the least synergistic effect relative to other combinations. From...
Read More
Posted by admin on Jul 31, 2015 in |
The bioactive components of Bryonopsis laciniosa fruits have been evaluated using GC/MS. The chemical compositions of the methanolic extract of Bryonopsis laciniosa fruits were investigated using Perkin-Elmer Gas Chromatography –Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis shows that the most prevailing compounds were identified as Oleic Acid, Hexanoic acid, 2-Ethylcyclohexanone, 2-Methyltetracosane, 2-Undecenal, 1,2-Benzenedicarboxylic acid, Ascorbic acid 2,6-dihexadecanoate, Octadecanoic acid, (2E)-2-Decenal.The compounds like Sulfurous acid, n-Nonaldehyde, 2-Hepten-3-ol, Decadienal, 3-Octenoic acid, 1-[2-(acetyloxy)ethyl]-3-oxooctyl acetate $$ acetic acid 3-acetoxy-5-oxo-decyl ester, 9-Octadecenoic acid, 2H-Pyran-2-one, Z,Z-4,15-Octadecadien-1-ol acetate were moderately present. The results confirm the presence of bioactive components which are known to exhibit medicinal value as well as pharmacological...
Read More
Posted by admin on Jul 31, 2015 in |
Larrea divaricata Cav. commonly known as “jarilla hembra” is used in folklore medicine as anti-inflammatory. There was no scientific evidence justifying the medicinal use of the aqueous extract for inflammation diseases. Nowadays, there are two principal types of anti-inflammatory drugs: Steroidal anti-inflammatory drugs (corticosteroids) and non-steroidal anti-inflammatory drugs (NSAIDs), which exert gastrointestinal ulceration and bleeding. In this work the preventive anti-inflammatory activity of L. divaricata aqueous extract (AE) in C3H/He mice was determined by carrageenan- induced inflammation and ear edema tests, also its toxicity on gastric tissues and on blood cells was studied. The chromatographic profile showed: nordihydroguaiaretic acid (NDGA) as majority compound. AE exerted anti-inflammatory action without inducing gastric or blood cell toxicities. Also, it could modulate anti-inflammatory and pro-inflammatory cytokines. NDGA was not the compound responsible of the anti-inflammatory action. This work rescues the potentiality of AE as a gastric and blood cells innocuous anti-inflammatory agent and confirms its ethno- pharmacological...
Read More
Posted by admin on Jul 31, 2015 in |
In today’s hectic lifestyle, such dosage form as plaster, attracts more attention of scientists and gives new opportunities to use it in medicine. The purpose of our study was to investigate consumer properties of transdermal patches, hold the commodity analysis of the dosage form and determine the prospectiveness of their use in modern pharmacotherapy. Market review has been conducted. Analyzed the producing countries of transdermal patches reported on the pharmaceutical market of Ukraine. An analysis of the active ingredients used in the TTS has been carried out. Modern classifications of transdermal therapeutic systems have been given. The approaches to creation of TTS have been investigated. The most important consumer properties of TTS have been...
Read More
Posted by admin on Jul 31, 2015 in |
Equisetum arvense, commonly known as field horsetail, is a perennial herb belonging to the genus Equisetum. Traditionally, Equisetum arvense is used as diuretic; anticoagulant, skin antiseptic, in kidney and liver related problems and gastric ulcers. E. arvense has also been suggested for the treatment of bone related disorders such as osteoporosis but the data is very scarce on its dose range and its influence on basic biochemical parameters. The present study was carried out to study its effect in female wistar rats at three levels of dose: L (30 mg/ kg of body weight), M (60 mg/ kg of body weight) and H (120 mg/ kg of body weight) for a period of 30 days. We report here complete blood count, LFT, KFT, blood glucose, total protein, and albumin and lipid profile. No toxic effects were observed in reference to clinical signs, body weight and organ weight. HBG, HCT, MCV, PLT, glucose and serum cholesterol, HDL and LDL level at H dose was found significantly different to Control group....
Read More
