Posted by admin on May 30, 2015 in |
The Ooctapéptido (LAN: 3-(2-Naphthalenyl)-D-alanyl-L-cysteinyl-L-tyrosyl-D-tryptophyl-L-lysyl-L-valyl-L-cysteinyl-L-threoninamide (2 → 7) disulphide acetate, angiopeptin acetate) cyclic covered gold nanoparticles (AuNPs) results in a complex (AuNPs-LAN) when reacted following the aqueous synthesis using Turkevich method for preparing colloidal gold nanoparticles followed by reaction of conjugation with the octapeptide LAN. The nanoconjugate was characterized by transmission electron microscopy high-resolution, UV-vis spectrophotometry, and by Zeta-potential. The objective of this study was to develop a method of capillary zone electrophoresis (CZE) to determine AuNPs and AuNPs-LAN in vitro. The reaction was I carried out by reduction of HAuCl4 with trisodium citrate, based on the synthesis of Turkevich-Frens-Kimling for colloidal Au at 90 °C where the plasmon resonance was detected at a wavelength of 526 nm, previously reported from 1954, 1976 and 2006, wavelength UV-vis nanoconjugate was 531 nm in which prolongation observed in migration times. We also describe part of the validation (linearity and precision) by capillary electrophoresis, arguing that it is a suitable method to quantify the gold nanoparticles and AuNPs-LAN complex. We conclude that the...
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Posted by admin on May 30, 2015 in |
Antioxidants prevent the damage done to the cells by free radicals that are released during the normal metabolic process of oxidation. Some of these free radicals include reactive oxygen species, hydroxyl, the superoxide anion, hydrogen peroxide and peroxyl which generate metabolic products that attack lipids in the cell membranes or DNA. These are associated with DNA damage, carcinogenesis and cellular degeneration related to aging and also contributes to heart diseases and arthritis. Researchers around the world have identified many natural constituents with antioxidant activity. Thus in the present work we have studied the synergistic antioxidant activity of lipoic, ferulic and ellagic acid which is naturally occurring compounds having medicinal properties by 1, 1-diphenyl-2-picrylhydrazyl assay. Highest antioxidant activity is reported for mixture of all the three compounds with 98% & 64% inhibition in both methanoilc and aqueous solutions followed by ferulic acid with 97% & 70% inhibition and mixture of ellagic and lipoic acid with 96% & 59% inhibition in both methanoilc and aqueous solutions. Thus from the results it...
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Posted by admin on May 30, 2015 in |
A simple, precise, specific and accurate high-performance thin-layer chromatographic method has been developed for the simultaneous determination of Emtricitabine (EMB) and Tenofovir (TEN) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica gel 60 F254, (20×10 cm) with 250 μm thickness using toluene: ethylacetate: methanol in the ratio (2:8:1 v/v/v) as mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 272 nm. The drugs were resolved satisfactorily with Rf values of 0.44 and 0.68 for EMB and TEN, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9999 and 0.9995 for EMB and TEN, respectively, in the concentration range of 40–400ng spot−1 for EMB and 200–700ng spot−1 for TEN. The method was validated for precision, robustness, specificity and accuracy. The limits of detection and quantitation were 20 and 40ng spot−1, respectively, for EMB and 100 and 200ng spot−1, respectively, for TEN. The proposed...
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Posted by admin on May 30, 2015 in |
In the present study, high performance thin layer chromatographic method was developed and quantification of Diosgenin from Albizia lebbeck. TLC auminium precoated with silica gel were used as the stationary phase in a twin see through glass chamber saturated with n-butanol: glacial acetic acid: water (4:1:1) mobile phase, performed at room temperature (25ºC ± 2). The Rf value of diosgenin was found to be 0.8. Linearity was found to be in the concentration range 2 to 10 µg/spot. The linearity regression analysis of calibration plots showed good linear relationship between peak area and peak height (r2= 0.996). The result showed that the chromatogram of Diosgenin was performed in 3D display of diosgenin and Overlay of diosgenin and ethanolic bark extract of Albizia...
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Posted by admin on May 30, 2015 in |
The aim of the present study is to identify the volatile, bioactive phytoconstituents present in the ethanolic extract of the leaves of Glochidion ellipticum Wt. (Euphorbiaceae) was carried out by using GC-MS analysis, while mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology and Wiley library. Twenty seven components from leaves of the above said plant were identified. The active principles with their retention time, molecular formula, molecular weight and concentration (%) in the ethanol extract of leaf of G. ellipticum are obtained. This is the first report of documentation of active constituents from leaves of Glochidion ellipticum. The research reveals the potential of G. ellipticum leaves as a good source of bioactive compounds such as esters, ethers, alkaloids, alkenes, fatty alcohols, terpenoids, amines, porphyrin and silicon compounds that justify the use of this plant for its various ailments by traditional...
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