Posted by admin on Mar 30, 2015 in |
Present study was designed to evaluate the antioxidant and hematoprotective activity of methanolic extract of saraca indica bark. Antioxidants and hematoprotective effect of saraca indica bark may be due to its polyphenolic compounds. Polyphenols act as reducing agents via scavenging of free radicals and chelation of transition metals. The hematoprotective activity of Saraca indica was studied in Phenylhydrazine induced anemic rats. Anemia was induced by an oral administration of Phenylhydrazine for a period of 7 days. Hgb concentration, RBC count, PCV, MCH, MCHC were analyzed as indices of anemia. Phenylhydrazine significantly decrease the Hgb, RBC and increased MCV and WBC. The Saraca indica was administered at the dose levels of 200mg/kg, 400mg/kg orally to the animals for 14 days. After 14 days of treatment with Saraca indica at the dose level of 400 mg/kg significantly reverse the above parameters and turn towards the normal value. These results support the traditional use of Saraca indica in the treatment of...
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Posted by admin on Mar 30, 2015 in |
The present investigation was carried out in five villages of the marginalized hill area block of Dewal in Chamoli district, regarding the uses of mild and native ethno-medicinal plants, by locals in their traditional health care system. The people residing in this area have been in isolation for centuries and possess good knowledge of phytotherapy. A total of 51 species of medicinal plants (both wild and cultivated) belonging to 30 families and 45 genera are being used in their folk system of treatment (locally known as ‘Gharelu Upchar’). These plants are used in crude form to treat various ailments like, cough and cold, stomach problems, skin diseases, dysentery, boils and pimples, anaemia asthma, cuts and wounds, various fevers, bone fractures, kidney/ gall stone problems and other such indispositions (Table 1.1 and 1.2). The main purpose of this study was to document the indigenous knowledge of Vaidyas and other knowledgeable persons of the area about the use of local flora, as household remedies for treating different...
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Posted by admin on Mar 30, 2015 in |
Present investigation provides evidence for complex formation of Ibuprofen (Ib) with Go-Ghrita (GG). Ibuprofen binary mixture in (1:0.5), (1:1), (1:2), (1:3) w/w proportions were prepared by adding to molten Go-Ghrita kept over a water bath at 65-70oC with continuous stirring. Organoleptic and Physico-chemical properties of GG (procured from Magan Sangrahalaya, Wardha) were as per the specifications given in Ayurvedic Pharmacopoeia (AP) and Indian Pharmacopoeia (IP). All proportions were subjected for FT-IR and in-vitro release behaviour (SGF, PH 1.2 for 2 Hrs and SIF, PH 6.8 for 7 Hrs) and on basis of it, optimized (1:1) w/w proportions were further analyzed and characterized for Differential Scanning Calorimetry, X-ray diffraction, Nuclear Magnetic Resonance and Scanning Electron Microscopy. Carbonyl stretching of isopropionic acid group to slight higher frequency 1.39 % cm-1 (increase) and (–C=O) linked (-OH) band stretching to slight lower frequency 1.09 cm-1 (decrease) shown in FT-IR spectra, supported by in-vitro release of Ibuprofen 99.16±0.17% of (1:1) w/w in sustenance form than remaining. Exclusion of polymorphic modification in X-RD, Slight lowering of...
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Posted by admin on Mar 30, 2015 in |
The most common cause of death is bone marrow failure. Chromosomal analysis is essential for the diagnosis, response to treatment and prognosis of leukemia. Conventional karyotyping which shows the structural, numerical and compound aberrations have improved the genetic diagnosis of leukemia. The bone marrow samples from patients (2-60yrs) of 53 patients (32 male and 21 female) suffering from various types of leukemia were collected. The patients were selected on the basis of clinical diagnosis and pathologically presenting blast cells in the peripheral smear. After photography all cells were analysed and printed photographs were taken for karyotyping and the chromosomes were banded, karyotyped and studied in detail for translocations, deletions, inversion, monosomy, trisomy and hyperdeploidy. It is observed that in all subtypes of leukemia male patients are more prevalent than female. Certain translocations t(8:21), t(8:15) in AML and Hyperploidy in ALL had better treatment outcome with aggressive chemotherapy. Hence younger age group were usually having t(4:11) in ALL , t(8:21) & t(15:17) in AML had better prognosis than other chromosomal...
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Posted by admin on Mar 30, 2015 in |
In this study, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were performed on a series of 73 experimentally reported natural flavonoid derivatives as AMV and HIV Reverse transcriptase (RT) inhibitors with pIC50 values ranging from 4.00 to 5.92. Based on alignment, highly predictive CoMFA and CoMSIA were obtained with cross-validated q2value of 0.760 and 0.696 respectively, and non-cross-validated partial least-squares (PLS) analysis with the optimum components of five showed a conventional r2 of 0.964 and 0.977 respectively. The CoMFA and CoMSIA models have been further validated for their stability and robustness using group validation and boot-strapping techniques and for their predictive abilities. The analysis of CoMFA contour maps provided insight into the possible modification of the molecules for better...
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