Posted by admin on May 30, 2015 in |
In the present work a simple, accurate and precise method has been developed and validated for the simultaneous estimation of Perindopril Erbumine (PDE), Amlodipine Besylate (AMD)and in their combined dosage form by UV Spectrophotometric Methods. The Method A employs estimation of drugs by simultaneous equation method (SEM) using 298.3 nm and 231.9nm i.e. λmax values of PDE and AMD respectively. Method B employs the estimation of drugs by Absorption Correction method (ACM) at 250.4nm for PDE where absorbance of AMD is zero. Absorbance correction method was based on the property of additivity of absorbances. At 250nm, Perindopril erbumine showed some absorbance while Amlodipine besylate showed zero absorbance. The method involved measurement of absorbance at two wavelengths 250 and 231 nm. For estimation of AMD, corrected absorbance was calculated at 231 nm due to the interference of PDE at this wavelength. Calibration curves were linear with correlation coefficient between 0.999 over the concentration range of 10-150μg/mL and 0.5-10 μg/mL for PDE and AMD respectively. The percent recoveries of the drugs...
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Posted by admin on May 30, 2015 in |
Armodafinil is an enantiopure of the wakefulness -promoting drug Modafinil, unique psycho stimulant recently approved by the US Food and Drug Administration for the treatment of narcolepsy; it is useful for treating excessive day time sleepiness. A simple, sensitive and accurate and economical spectrophotometric method has been developed for the estimation of Armodafinil in bulk and pharmaceutical dosage forms. This method is based on oxidative coupling reaction of 3-methyl-2-benzathiazoline hydrazone (MBTH) in the presence of ferric chloride (Fecl3). An absorption maxima was found to be at 596nm with the solvent system methanol: water (3:97). The drug follows Beer-Lambert law in the range of 10- 50µg/ml with correlation coefficient of 0.999. The percentage recovery of Armodafinil in pharmaceutical dosage form is in between 96- 106. Results of the analysis were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. The proposed method is simple, rapid and suitable for the routine quality control...
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Posted by admin on May 30, 2015 in |
This article presents the results of studies on determination of optimal parameters of recombinant strains A/NYMC X-217 (H3N2) and B/NYMC BX-49 of influenza virus cultivation in chicken embryos. It is found that a maximum virus accumulation is formed under the following cultivation conditions: age of chicken embryos is 9-11 days in case of inoculation of the allantoic cavity, inoculated virus dose — 1,000-10,000 EID50, temperature and time of incubation — (34 ± 0.5) ºС and 48 h, respectively. If the cultivation parameters are satisfied, it is possible to obtain a highly active virus-containing material with infectious and hemagglutinating activity, as well as a content of hemagglutinin not less than 7.0lg EID50/ml, 1:256 and 7.0μg/ml, respectively, that is enough to prepare a trivalent inactive seasonal influenza split...
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Posted by admin on May 30, 2015 in |
The antioxidant potential of the aqueous and hydro/ethanolic (ethanol/water) extracts of Crinum jagus were explored considering their total phenolic, flavonoid and proanthocyan contents, 1.1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferrous ions chelating ability and nitric oxide radical inhibiting activity. Hydroethanolic extract has exhibited the highest total polyphenols and flavonoids content as well as the highest DPPH inhibition value (56.71 ± 0.05 % at 320 µg/mL) and ferrous ions chelating ability (60.25 ± 0.22 % at 6 mg/mL), when the aqueous extract showed the highest proanthocyanes content, nitric oxide inhibiting activity comparable to that of ethanol/water extract (respectively 64.52 ± 0.03 % and 65.90 ± 0.43 % with 320 µg/mL extract). The IC50 values of the DPPH and the ferrous ions inhibition were respectively 25.21 µg/mL and 4.69 mg/mL; the IC50 value of nitric oxide inhibiting ability was 16.48 µg/mL and 16.14 µg/mL for the aqueous and hydroethanolic extracts respectively. The results indicate that, Crinum jagus might possess effective in vitro antioxidant activity which could be more marked with ethanol/water...
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Posted by admin on May 30, 2015 in |
Unani and Ayurvedic heath care system is considerable areas of medical treatment in Bangladesh where patient can avail it from different Govt. or private hospital. This cross-sectional study was undertaken to find out the satisfaction of patients attending in this health care system from January to April 2014. A total of 240 respondents were interviewed with an interviewer administered semi-structured questionnaire. Among the respondents majority (54.2%) of them was aged ranging from 21- 40 years. The study revealed that more than 71% respondents were married and majority of them (55.4%) have history of use of Unani/Ayurvedic medicine before. The study found that majority (79.6%) of the respondent has found satisfactory and good quality behavior from the doctor of these systems and it was also found that a good number of respondents (more than 80%) commented doctor’s service as satisfactory and good. It was found that more than 80% respondent got satisfactory and good behavior from pharmacist and concerned stuff of the hospital. Regarding health service delivery system of the...
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