Posted by admin on Feb 26, 2015 in |
Metal oxide in the nanorange acquires distinctive properties that depend on size, chemical composition and surface chemistry. Among the metal oxide nanoparticles, the zinc oxide nanoparticles (ZnO NPs) are commonly used for their antimicrobial properties. ZnO nanomaterials are incorporated into a variety of skin coatings because of their antimicrobial and/or antifungal properties. Most of the future therapeutic applications of NPs are based on intravenous/oral administration. Experiments on their interaction with blood components especially erythrocytes are of immense importance, if the nanoparticles are to be administered intravenously. Haemolytic potential of ZnO NPs was assessed spectrophotometerically as well as by phase contrast microscopy. In our study, interaction of different concentrations of ZnO NPs with erythrocytes revealed absence of hemolysis by spectrophotometric method. On the other hand, phase contrast microscopic examination revealed concentration dependent clustering of erythrocytes. The detailed investigation about interaction of erythrocytes with ZnO NPs is needed before their clinical...
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Posted by admin on Feb 26, 2015 in |
Optimization of total carotenoids production is dependent on various cultural and physical parameters for pigmented yeasts. The carotenoid production of the red yeast Rhodotorula mucilaginosa MTCC11835 was optimized using response surface methodology. The carotenogenesis in yeasts is known to be affected by different nutritional factors and environmental stress factors. Effect of various growth parameters like carbon source (glucose; glucose:glycerol (1:2); maltose), nitrogen source (peptone; casein hydrolysate; yeast extract), pH (3.5;5.0; 6.5) and stress factors (Tween20; hydrogen peroxide) were studied for the maximum yield of carotenoids. The yeast was shown to produce high amount of total carotenoids (21.77 mg g-1) in a medium containing glucose: glycerol (1:2), peptone, 0.5% Tween 20, 2.5 mM H2O2 and pH...
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Posted by admin on Feb 26, 2015 in |
Different prokineticin receptor antagonists, based on the triazinedione scaffold, were synthesized by a new efficient method. Here we demonstrated that 5-benzyltriazinediones substituted in position para of the benzyl group with halogens provide compounds endowed with interesting selectivity for the Prokineticin receptor 1 (PKR1). BRET technology indicates that such substitution results in increased affinity for the PKR1. The affinity for PKR2, always in µM range, was never significantly affected by the para-halogen-benzyl pharmacophores. The analog bearing a para-bromobenzyl pharmacophore (PC-25) displayed the highest affinity for PKR1 (~18 times higher than the reference PC-1 that bears apara-ethyl benzyl group) and the highest selectivity (~300 times). The other halogen substituted analogs (PC-7, PC-18 and PC-35), showed selectivity for PKR1 more than 100 times higher than for PKR2. Using transgenic mice lacking one of the two PKRs we demonstrated that all these compounds were able to abolish the Bv8-induced hyperalgesia in mice still expressing the PKR1 at doses lower than those necessary to abolish hyperalgesia in mice expressing only the PKR2. The dose...
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Posted by admin on Feb 26, 2015 in |
Inflammation is common and essential protective response to the harmful stimuli such as infectious agents, antigen-antibody reactions, thermal, chemical, and physical agents, and ischemia. The oxidative stress is produced by free radicals, predominately reactive oxygen species (ROS), which cause tissue damage from oxidative stress. Antioxidants play an important role in neutralizing such free radicals. Research in recent decades has shown widespread pharmacological effects of A. sativum and its organosulfur compounds especially Allicin. Studies carried out on the chemical composition of the plant show that Allicin is an important constituent of the plant and is the most important alkaloid that is generally claimed to be responsible for their beneficial effects which is known to possess antioxidant and anti-arthritic activity. Hence an attempt was made to investigate its antioxidant and anti-arthritic...
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Posted by admin on Feb 26, 2015 in |
Adrenomedullin-2 or Intermedin (ADM2/IMD) is newly identified novel hormone belongs to peptide family like AM, calcitonin, CGRP, amylin and it is widely distributed in various tissues including ovaries, uterus & placenta. The aim of present study was to investigate the role endogenous ADM2 in fetoplacental development in pregnant rats during late gestation period through infusion of ADM2 antagonist. For this study we have infused ADM2 antagonist (ADM217-47) at 50 and 200 µg / rat / day exposed continuously by inserting osmotic mini pumps to pregnant wistar rats on gestational day 18 and were sacrificed on gestational day 22.The results showed that infusion of ADM2 antagonist significantly decreases (P<0.05) the total sac weight, fetoplacental weights, length of the fetus and also the serum progesterone & estrogen levels. High molecular DNA fragmentation was observed in ADM217-47 treated placental tissues and in immunohistopathological studies apoptotic cells (pyknosis & karyorrhexis cells) and active caspase-3 positive immunoreactivity cells were observed in labyrinth zone of placental tissues. In western blot analysis the levels of caspase-3...
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