Posted by admin on Mar 30, 2015 in |
Tinidazole is a synthetic antiprotozoal and antibacterial agent which is practically insoluble in water.Oral dose of tinidazole is very high (2gm) owing to its limited solubility. Present research focuses on enhancement of solubility of tinidazole using the technique of solid dispersion which is reported to be one of the simple technique for solubility enhancement using various grades of Gelucire and PEG 6000. The ratio of drug and Carrier was decided based on phase solubility study. Solid dispersions were prepared by different techniques such as physical mixture, kneading, co-precipitation and closed melting method.Compatability between drug and carrier was checked by FTIR studies. Efficiency of solid dispersions was checked by conducting saturation solubility testing in water. Multimedia dissolution was carried in different media’s such as Water, 0.1 N HCl and Phosphate Buffer after checking drug content. The formulations were found to enhanced solubility and dissolution as compared to pure...
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Posted by admin on Mar 30, 2015 in |
An HPTLC method was developed for qualitative and quantitative estimation of catechin, quercetin and β-carotene in the marketed and in house polyherbal formulation Maharishi Amrit Kalash (MAK-5). The method was developed using precoated HPTLC silica gel 60 F254 as stationary phase and toluene: ethyl acetate: formic acid (12:12:2, v/v/v) as mobile phase for catechin and quercetin and petroleum ether (40-600C): acetone (17:3, v/v) for β-carotene. Detection and quantification were performed densitometrically at 200nm for catechin and quercetin and 430 nm for β-carotene. The Rf values of catechin, quercetin and β-carotene are 0.29 ± 0.01, 0.49 ± 0.01 and 0.70 ± 0.01 respectively. The percentage content of catechin, quercetin and β-carotene in marketed and in house polyherbal formulation are 0.223%w/w, 0.099%w/w and 2.313%w/w and 0.114%w/w, 0.141%w/w and 2.486%w/w respectively. Linearity was observed in the concentration range of 100-800 ng/spot for catechin and quercetin and that of 100-600ng/spot for β-carotene. Quantification of catechin, quercetin and β-carotene was found to be achieved by HPTLC fingerprinting from marketed and inhouse polyherbal...
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Posted by admin on Mar 30, 2015 in |
In the present work, we have synthesized a new analoges of monocyclic β-lactam (2-(3-(2, 4-dichlorophenoxy)-2-(substituted aryl) – 4-oxoazetidin – 1 – ylamino) – N – (pyridin-2-yl) acetamide) derivatives in the presence of triethyl amine (TEA) and phosphorus oxychloride (POCl3) under classical method by using Dichloromethane (DCM) as a solvent. The designed compounds 4(a-l) were prepared by Staudinger reaction ([2+2] ketene-imine cycloaddition reactions). In which an azetidin-2-one motif connects with pyridine-2-acetamide nucleus with two aromatic rings. The target compounds were screened for in vitro antibacterial activity against clinically relevant Gram-negative (Escherichia coli and Klebsiella pneumonia) and Gram-positive species (Bacillus subtilis, Proteus vulgaris and Staphylococcus aureus). The obtained results have demonstrated that all the synthesized imidazole-conjugated monocyclic β-lactam derivatives showed good antibacterial activity. Particularly the compounds 4e and 4l found to be effective in P.vulgaris as equal to reference ampicillin and other compounds showed moderate to good activity against five human bacterial pathogens. All these compounds have been characterized by IR, 1H-NMR, 13C-NMR, Mass spectrometry and Elemental...
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Posted by admin on Mar 30, 2015 in |
The methanolic extract of whole plant Coldenia procumbens was screened for its analgesic activity. The crude extract was assessed for central analgesic effect using in-vivo methods like hot plate method, tail-flick method, and tail-immersion method at two different doses. The extract at the dose of 200mg/kg body weight showed significant (p<0.001) analgesic effect comparable to the standard drug Pentazocine at the dose of 10mg/kg body weight. The peripheral analgesic was assessed by acetic acid-induced writhing test in mice. The methanolic extract at a dose of 200 & 400mg/kg body weight found to show significant (p< 0.001) analgesic effect comparable to the standard drug Aspirin at the dose of 20mg/kg body...
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Posted by admin on Mar 30, 2015 in |
The aim of the present study was to isolate, identify and to analyze the phylogenetic characteristics of the potent actinobacterial strain VL-RK_05 isolated from Mango orchards of Vissannapet, Krishna Dist, Andhra Pradesh. Soil samples collected were pre treated with calcium carbonate and used for isolation of potent Actinobacterial strain designated as VL-RK_05. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S r RNA sequence based analysis. Phylogenetic tree was constructed using the Molecular Evolutionary Genetic analysis version 5. Phylogenetic analysis of 16S r RNA sequencing revealed that the strain is closely related to Rhodococcus erythropolis and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Shigella flexneri, Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida...
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