Posted by admin on Apr 30, 2015 in |
Eurycoma longifolia has been widely used by society as an aphrodisiac especially in Indonesia, Vietnam and Malaysia. However, only few studies have been published related to the herb-drug interaction. Previous study showed that the standardized extract of E. longifolia affected phase I rosiglitazone metabolism especially in the old male rat hepatocytes for both diabetic and normal rats. The present study evaluated the possible mechanism of E. longifolia extract in affecting of phase I rosiglitazone metabolism in the old normal male rat hepatocytes. Experiment was performed used ten cellular stimulants/inhibitors. Old normal male rats were divided in ten groups (n = 6) and the hepatocytes were isolated using perfusion technique. Each group had a negative control (in the presence of vehicle), a positive control (in the presence of cellular inhibitors/stimulants) and 6 test subgroups (in the presence of cellular inhibitors/stimulants and 0.001-100µg/mL of E. longifolia extract). Effect of E. longifolia extract on phase I rosiglitazone metabolism was determined by colourimetric method of Nash at 415nm. The results showed the significant...
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Posted by admin on Apr 30, 2015 in |
Studies on plant secondary metabolites have been increasing over the last 70 years. These molecules are known to play a major role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals. Role of primary metabolites in basic life functions such as cell division and growth, respiration, storage, and reproduction have been described in modern chemistry and biology. In biology, the concept of secondary metabolite can be attributed to Kossel. He was the first to define these metabolites as opposed to primary ones. In the present study, the bioactive secondary metabolite myricetin was identified and isolated from in vivo and in vitro tissue of Cochlospermum religiosum a critically endangered medicinal plant. Myricetin is a naturally occurring flavonol found in many plants like grapes, berries fruits, vegetable, herbs as well as other plants. Myricetin has wide array of biochemical properties, such as anti-neoplastic, anti-carcinogenic antioxidant activity, anti-inflammatory effects. Myricetin was isolated and identified from leaf and callus by using different techniques such as...
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Posted by admin on Apr 30, 2015 in |
Reactive oxygen species (ROS) formation from fertile volunteer donor measured by thiobarbuteric acid reactive substances (TBARS). Spermatozoal suspension was treated or not with nicotine and supplemented or not with Mn2+. The same protocol followed when we used ferrous ascorbate promoter system and calculates the Cmax and kc value of the reaction using Lineweaver-burk double reciprocal plot. For comparing the antioxidant status Mn2+ with other trace elements employing various concentrations (10-100µM), production of malondialdehyde (MDA) calculated. Effect of lipid peroxidation (LPO) upon membrane integrity monitored by performing hypo-osmotic swelling test (HOS). Extent of LPO was measured for one-hour intervals of 10 minutes each, TBARS production found to increase gradually. Supplementation of Mn2+ to nicotine treated or not spermatozoal suspensions significantly lower the TBARS production in different concentration of promoter system (ferrous sulphate and ascorbic acid; 1:5). According to Michaelis-Menten kinetic it was observed that there was linear and positive correlation between amounts of malon-dialdehyde produced (vi) and increase in substrate concentration (S). Nicotine treated or not supplemented or not with...
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Posted by admin on Apr 30, 2015 in |
We have developed a novel LAMP-based molecular marker for easy and fast identification of Podophyllum hexandrum Royle (Indian May apple). It is an important medicinal plant valued all over the world. It have both anti-cancer (pro-oxidant ability) and radioprotective (antioxidant ability) effects respectively. The LAMP-based molecular was constructed based on genomic DNA of P. hexandrum that amplified by RAPD. A RAPD amplicon common to all accessions, was eluted, cloned and sequenced. Based on these sequences in-house specific LAMP primers were synthesized. This method employs by a Bst DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the P. hexandrum genomic DNA. In LAMP reaction the amplification was carried out in thermal cycler and water bath at 60°C for 60 min, which was followed by monitoring DNA amplification with the naked eye through color changes by standardizing SyBr Green® I nucleic acid stain. The data showed confirmatory results in both thermal cycler and water bath. Since, the assay method is...
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Posted by admin on Apr 30, 2015 in |
To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabean, the present study was intended to evaluate the in-vitro immunomodulatory activity of dry seeds of Glycine max. L. Effect of ethanolic and methanolic extracts of soyabean was evaluated at various concentrations (800μg/ml to 6.25μg/ml) for secretion of various mediators. Immunomodulatory activity of both extracts was examined on murine macrophage phagocytosis by Nitroblue tetrazolium (NBT) dye reduction, nitric oxide, lysosomal enzyme activity and myeloperoxidase activity. Proliferation of spleenocytes and bone marrow cells was evaluated by 3 (4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) as mitogen. Both aqueous and acetone extracts presented immunomodulatory activity in peritoneal mouse macrophages, bone marrow cells and spleenocytes without clear dose response relationship. In-vitro phagocytic index and proliferation index showed significant results and thus proving the need for confirmation through in-vivo...
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