Posted by admin on Apr 30, 2015 in |
Mallasindura (MSL), a widely used ayurvedic preparation is used for the treatment of bronchial asthma. It contains rasa –mercury, Rasakarpura -mercurial compound, Bali –purified and processed sulphur and Malla-purified arsenic oxide with herbal juices. The liver function parameters of rat plasma were studied after chronic administration of MSL. The animals used to conduct this experiment were albino rats (Rattusnovergicus: Sprague-Dawley strains) and the drug was administered per oral route. The administered dose of the drug was 100mg/kg body weight, once daily up to 90 days for the whole study. There were forty rats for four randomized groups, equally of both sexes. Among them one male and one female group were used as test. In case of male rats, there was increase in total protein and albumin content, which was statistically highly significant with a p value of 0.001. Along with this, a statistically highly significant declining trend was followed for bilirubin, sGPT, sGOT activities (p values are 0.001 and 0.009 respectively). In case of female rats, a statistically highly...
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Posted by admin on Apr 30, 2015 in |
Ficus dalhousiae Miq. is a plant found in Andhra Pradesh and Tamil Nadu on rocky hill top of dry deciduous forests. As it possesses antioxidant property wherein it reduces the elevated levels of ROS, the intent of the present study was to evaluate the Gastro protective effect of Ficus dalhousiae Miq Root ethanolic extract (FDREE) by means of Indomethacin and Cold Restrain stress induced Ulcers in Albino rats. Indomethacin 5mg/kg body weight p.o; for five days and Cold restraint stress models, were used for inducing gastric ulcers in rats. Biochemical parameters such as Glutathione, Malondialdehyde, acidity, Gastric volume, gastric pH and Ulcer index were determined in order to assess the gastro protective activity of FDREE in both the models. Treatment of rats with Indomethacin and subjecting them to Cold restraint stress (CRS) elevated the levels of Gastric volume, acidity, Glutathione, Malondialdehyde and gastric pH in negative control group in comparison with normal group. The elevated levels were significantly reversed when treated with standard drug Ranitidine 50 mg/kg body weight...
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Posted by admin on Apr 30, 2015 in |
A very sensitive, rapid and simple Ultra High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) method has been developed and validated for the simultaneous estimation of Metformin Hydrochloride and Sitagliptin Phosphate in standard and tablet formulation. The chromatographic separation of the drugs was achieved on a Hypersil Gold C18 analytical column (150x3mm, 5µ) with an isocratic elution of methanol and water with 0.2% formic acid (48:52 v/v) at a constant flow rate of 500µL/min on a UHPLC and the retention time of Metformin Hydrochloride and Sitagliptin Phosphate were 1.40±0.01 and 2.38±0.01 min respectively. Elutes were detected online on an Orbitrap mass spectrometer in ESI (+) mode. The method was validated according to ICH guidelines. The calibration plot was linear with correlation coefficient (r2) of 0.9998 for Metformin Hydrochloride in the concentration range of 0.04-0.28ng/mL and 0.9992 for Sitagliptin Phosphate in the concentration range 0.04-0.28 ng/mL respectively. The LLOD of Metformin Hydrochloride and Sitagliptin Phosphate were 0.2 and 0.02pg/mL respectively. This method was validated with respect to linearity, accuracy, precision, specificity and...
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Posted by admin on Apr 30, 2015 in |
Empirically dry fallen of Artocarpus altilis leaves are widely used as a traditional medicine, but the green leaves that have been widely studied. Therefore, this research was conducted to compare the metabolite profile of green leaf, attached yellow, fallen yellow, fallen dry, and fermented green leaves. Comparison of flavonoid content was conduted by HPLC. Extraction was performed using Soxhlet apparature, fractionation by VLC and setrifugal chromatography. HPLC analysis of various leaves showed that the fallen dry leaves contained the highest flavonoid compound. Three compounds were isolated, compound 1 characterized Uv-Vis, IR, 1H-NMR, 13C-NMR spectroscopies and identified as sitostenone. Compound 2 showed UV spectrum typical of flavonoid with M – 392 and tentatively identified as flavon with two prenyl substituents. Coumpound 3 was identified as b-caroten based on its UV spectrum and chromatographic...
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Posted by admin on Apr 30, 2015 in |
Background: Vitamin D3 deficiency continues to be an unrecognized epidemic in many populations around the world. Vitamin D is important for the absorption of calcium, and bone formation and maintenance. Objective: This study was carried out with an objective of evaluating the prevalence of low Vitamin D levels (insufficiency and deficiency) in female office workers and housewife’s in Geetanjali medical college and hospital, Udaipur. Material & method: This study was carried out in Geetanjali hospital and medical college, a retrospective study conducted on 50 working and 50 housewife’s subjects between age group 18-32 years. Body mass index (BMI) was calculated. Serum calcium, serum phosphorous, and alkaline phosphatase were determined by a fully-auto-analyzer. The serum level of Vitamin D3 was measured using the electro chemiluminescence immunoassay methodology. Results: The difference in the BMI between the office workers and housewife’s was statistically no significant. Vitamin D3 was 18.72 ± 6.97ng/ml (SEM0.987) in office worker’s and 9.94 ± 6.14 ng/ml (SEM 0.869) in housewife’s with a highly significant (p<0.0001) statistical difference. Conclusion: ...
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