Posted by admin on Jan 31, 2015 in |
This study was performed to determine the anti-ulcer effects of ethanol extract of Parkia speciosa on Indomethacin induced peptic ulcer in Albino rats. Rats of female sex weighing between 150 g – 200 g were used. Rats were divided into six groups with six rats in each group. Omeprazole (20 mg/kg) were used as a standard drug for this study. Parkia speciosa extract were given in three different doses comprised of 100 mg/kg, 200 mg/kg and 400 mg/kg. Pre-treatment were done for 14 days and at the end of the 14th day, rats were kept fasted for 24 hours before administration of Indomethacin at 30 mg/kg. Administration of drugs was done orally. At the end of the study, rats were sacrificed and stomachs were open and stored in 10% formalin solution. The acidity of gastric juice, gastric mucosal lesion and histological changes were studied. Extracts of Parkia speciosa showed a significant (P<0.05) reduction in acidity of gastric juice as compared to the ulcer control group. Two doses of Parkia...
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Posted by admin on Jan 31, 2015 in |
Bacopamonnieri L. Pennell (family: Sclorphulariaceae) is a reputed drug of Ayurveda. The present study was undertaken to investigate the antidepressant activity of alcoholic extract of B. monnieri was studied at doses of 40 mg / kg and 80 mg / kg against TST and FST mice model of depression. Dose dependent reductions in immobility time were observed in TST and FST mice model of depression. The results showed that the alcoholic extract decreased immobility time with the increase swimming time. Ethanolic extract (40, 80 mg / kg, p.o.) (P<0.01) significantly reduced the immobility time by 10 and 15 seconds as compared to the immobility time of control in both the screening models. Imipramine at doses of (20 mg/kg, p.o.,) was selected as reference standard. These results suggested the anti-depression activity of the plant extract. The effect of the extract was 5-20 times less potent than standard imipramine group which was used as a standard. It may be concluded that the alcoholic extract of B. monnieri produces an antidepressant like...
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Posted by admin on Jan 31, 2015 in |
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantization of Cefixime (CFXM) and Ofloxacin (OFLO) in tablet preparation. Chromatographic separation was carried out in a Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm; 5 μm particle size) of Agilent Technologies with simple mobile phase composition of 25 mM KH2PO4 in water (pH 4.63, maintained by dil Phosphoric acid) and Methanol (65:35, v/v) at a flow rate of 0.5 ml min-1 with injection Volume of 20 µl where detector was set at 288 nm with a total run time of 10 mins. The method was linear over the concentration range of 20-100, μg ml-1 for both of CFXM and OFLO with a correlation coefficient of 0.999 and 0.999 respectively. Limit of quantifications (LOQ) of 5.53, 5.24 and limit of detections (LOD) 1.82, 1.73 μg ml-1 for CFXM and OFLO respectively. Accuracy and precision values of both within-run and between-run obtained from six...
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Posted by admin on Jan 31, 2015 in |
Whole plant parts, fruits, roots, bark, peel, seeds and pulp of Carica papaya are known to have medicinal properties. It has been used for treatment of numerous diseases like warts, corns, sinuses, eczema, cutaneous tubercles, blood pressure, dyspepsia, constipation, amenorrhoea, general debility, expel thread worms and stimulate reproductive organs. Ayurvedic literature reveals that papaya leaf extract has haemostatic properties and recent studies revealed its ability on platelet augmentation in cyclophosphamide induced thrombocytopenia rat model. Pilot studies done in dengue patients with leaf juice revealed the effect of leaf juice on elevating white blood cells, platelet count and recovery without hospital admission. Hence, in the current study, an effort was taken to study the phytochemical profile of papaya leaf extract using Liquid Chromatography-Mass Spectroscopy (LCMS). Aqueous extract of young leaves were taken and subjected to LCMS analysis for phytochemical profiling using Water and acetonitrile as mobile phase. On LCMS analysis followed by integrated library search, 21constituents were identified and it included pharmacologically active phyto compounds, alkaloids, phenolics, flavonoids and also,...
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Posted by admin on Jan 31, 2015 in |
The present investigation deals with the determination of in vitro antioxidant activity as well as the phenols and ascorbic acid content in in vivo (leaf, stem, root) and in vitro (unorganised static callus cultures) plant parts of Pluchea lanceolata. Unorganised static callus cultures (eighteen months old) were grown on MS medium supplemented with NAA (1.0 mg/l) and BAP (0.5 mg/l) using leaf explants. Antioxidant activity was analysed in terms of DPPH radical scavenging assay, total phenolic contents were estimated by folin ciocalteau phenol reagent method and ascorbic acid (vitamin c) was analysed by 2, 4-dichlorophenol indo-phenol dye method. Antioxidant potential was found to be maximum (93.55 %) in leaf parts and minimum in stem (54.74 %). Lower IC50 value indicates high antioxidant activity which was maximum in stem (665.90±0.23) and minimum in leaf (123.37±0.16). Total phenols and ascorbic acid were also higher in in vivo tissues (leaf) as compared to in vitro tissues. In the present study excellent DPPH radical scavenging activity (RSA) was found in all extracts of...
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