Posted by admin on Jun 30, 2015 in |
A rapid, sensitive and specific RP-HPLC method was developed and validated for determination of Rosuvastatin Calcium in bulk and tablet dosage form. Chromatography was carried out on a Enable C18G (250 x 4.6 mm i.d.,5µ) column using filtered and degassed mixture of acetonitrile and water in the ratio of 75:25 % v/v as mobile phase at a flow rate of 0.6 ml/min and effluent was monitored at 252 nm. The method was linear over the concentration range of 5 – 40 µg/ml with a correlation coefficient of 0.999. The retention time of the drug was 3.097 mins. The proposed method was validated by determining sensitivity, accuracy, precision, robustness studies. The developed method was effectively applied to tablets of Rosuvastatin Calcium and the % assay of the drug was found to be 99.98 %. The method is simple, rapid, accurate, precise and reproducible and hence can be applied for routine quality control analysis of Rosuvastatin Calcium in pure and tablet dosage...
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Posted by admin on Jun 30, 2015 in |
This study aims to utilize natural palm oil in the formulation of liposome and evaluate its stability and degradation via the 1H NMR technique. In this study, the conventional method was used to prepare liposome consisting 0, 5, 10, 15, 20 and 25% of palm oil in their structure, and the liposome were characterized in vitro afterwards. The chemical degradation of liposome in the presences of blood component was detected using 1H NMR after incubation period of 2 hours, one day and one week. According to 1H NMR spectrums, samples that lack palm oil or those exceeding 15% palm oil concentration underwent decomposition in the first 2 hours, while samples that were loaded with 5,10 and 15% or palm oil showed no such degradation. However, after being incubated for a day with serum component, the samples loaded with 10 and 15% palm oil remained stable and did not succumb to degradation. It can be surmised that this approach is a viable analytical concept based on the chemical characteristics and...
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Posted by admin on Jun 30, 2015 in |
A series of novel 3-(substituted aryl)-1-(5-methylfuran-2-yl)prop-2-en-1-one derivatives (3a-k) were synthesized from commercially available 2-acetyl-5-methylfuran and various substituted aromatic aldehydes in presence of anhydrous potassium carbonate in ethanol solution and by microwave irradiation method with good yield compared to conventional method. Anhydrous potassium carbonate has been utilized as safe, inexpensive and efficient basic catalyst for synthesis of chalcones. The structures of the all synthesized compounds have been established on the basis of IR, Mass, 1H NMR and 13C NMR. The antimicrobial activities of these derivatives were evaluated by quantifying the minimal inhibitory concentration (MIC) against various microbial strains such as Escherichia coli [ATCC-9637], Staphylococcus aureus [ATCC-25923], Klebsiella pneumonia [ATCC-13883], Candida albicans [ATCC-28366], Rhizopus arrhizus [ATCC-11145] and Aspegillus niger [ATCC-26036]. Among the tested compounds, compound 3h exhibited strong microbial activity against most of the microbial strains except A....
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Posted by admin on Jun 30, 2015 in |
Background: Nephrotoxic Acute Kidney Injury (AKI) is a common condition associated with considerable morbidity and mortality. Acute exposure to potentially nephrotoxic drugs requires early appraisal of the extent of renal injury to determine the need for specific interventions. Hence this study was designed to evaluate two urinary biomarkers for the early diagnosis of AKI due to cisplatin chemotherapy, including Neutrophil gelatinase-associated lipocalin (uNGAL) and Cystatin C (uCysC). Methods: Urinary biomarker levels in cisplatin treated cancer patients were estimated before and at 2hrs, 6hrs, 12hrs, 24hrs and 48hrs after cisplatin administration. Diagnostic performances of biomarkers were studied by ROC analysis with AUC and predictive values. Results: uNGAL was identified as the earlier biomarker of AKI induced by cisplatin as it detected kidney injury as early as 2hrs after the exposure of nephrotoxic drug with an AUC of 0.8, which is 46hrs before the elevation of serum creatinine. uCysC detected AKI 6hrs after cisplatin administration with AUC 0.73. Conclusion: Indication of AKI by biomarker elevations can provide an early warning signal...
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Posted by admin on Jun 30, 2015 in |
In African traditional medicine, Ageratum conyzoides L. has been used as purgative, febrifuge, anti-ulcer and wound dressing. A. conyzoides contains pyrrolizidine alkaloids, a class of hepatotoxic, genotoxic and carcinogenic phytochemicals. This study aims to evaluate the genotoxicity of total alkaloids of A. conyzoides leaves by the alkaline-comet assay.The comet assay was done on human prostate cancer (PC3) cells and on mice PBMC cells. Our results have demonstrated that, in vitro, the hydroalcoholic extract (200 µg/mL) and total alkaloids (200 µg/mL) of A. conyzoides, which have shown the high cytotoxicity on PC3 cells with MTT assay, increased significantly (p<0.001) DNA damage index (DI) and DNA damage frequency (DF) of PC3 cells. The non-alkaloids and the methanolic extract of A. conyzoides had no effect on DI and DF. In vivo, the administration of total alkaloids of A. conyzoides at 750 mg/kg for 3 days increased significantly (p<0.01) the DI and the DF of mice PBMC cells. Thus, in this study, by in vitro and in vivo comet assay test, we have...
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