Posted by admin on Mar 30, 2015 in |
Simple, sensitive, precise, accurate, and specific reversed –phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of levofloxacinin pharmaceutical tablets. Levofloxacin is a third generation fluoroquinolones with a wide spectrum of action against gram-positive and gram-negative bacteria, anaerobic microorganisms and atypical pathogens. It is pure (-)-(S)-enantiomer of the racemic drug substance ofloxacin, which was introduced in 1997. Isocratic chromatography was performed on a C18 column with acetonitrile‐methanol‐phosphate buffer 0.1M15:25:60 (v/v/v) as mobile phase at a flow rate of 1 ml/min. UV detection was set at 287 nm. The fluorescence detector was set at excitation/emission wavelengths of 300/500 nm. The method was validated with respect to accuracy, linearity, precision, and selectivity. All the parameters examined met the current recommendations of U.S.P (30) for analytical method validation. The method can be reliably used for routine quality control analysis and to determine the levofloxacin content of marketed...
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Posted by admin on Mar 30, 2015 in |
Main aim of this study was to find out relation of CYP2D6 polymorphism with tramadol action in post herpetic neuralgia (PHN) patients. This non- responders versus responders study mainly includes 246 patients (age group 20-80 years) of PHN, assigned into two groups depending upon their response to tramadol treatment at the end of 2nd week of drug therapy. On the basis of CYP2D6 polymorphism, an allodynia and pins and needles significant interactions were found with CYP2D6*2 allele between time, group and metabolizers while with*4 and *10 alleles no interaction observed. Finally, results not showing role of CYP2D6 polymorphism in the development of neuropathic symptoms in PHN patients...
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Posted by admin on Mar 30, 2015 in |
: Thiosemicarbazobes are the compounds that possess wide range of biological activities. Presently the areas in which thiosemicarbazones receiving more attention is its use against cancer. Reports from the SAR studies confirms that the thiosemicarbazones with heterocyclic moiety shows carcinogenic potency. These are potent ribonucleotide reductase inhibitor. Adamantyl derivatives is also having interesting biological properties and most promising towards anticancer activity. The value of adamantyl group in drug design is multidimensional. A novel series of compounds of thiosemicarbazones were prepared by conjugating adamantyl group and using aromatic substitution. The various substitutions on thiosemicarbazones were reported as anti cancer activity and adamantyl group also show antiproliferative activity against some cell lines, so in present work 20 compounds were prepared on the basis of best results of QSAR studies and they were screened for anticancer activity using cell line studies. The results were compared with standard drug doxorubicin, 3 compounds show more inhibitory action than standard...
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Posted by admin on Mar 30, 2015 in |
A series of novel unsaturated fatty acid based b-hydroxy 1,2,3-triazole hybrids were designed and synthesised in excellent yields by esterification, epoxidation of oleic, ricinoleic and 10-undecenoic acids followed by azidolysis and “click” reaction with different alkynes. All the b-hydroxy 1,2,3-triazole hybrids were characterised by ESI-MS, IR and NMR spectra as well as HRMS analysis and were screened in vitro for their anticancer activity against selected four human cancer cell lines (DU-145, HeLa, MCF-7 and A549) in comparison with doxorubicin, a standard anticancer drug. Among all the triazoles, methyl oleate with-CH2OH as 1,2,3-triazole side chain (1dii) exhibited good anti cancer activity against DU-145, HeLa, MCF-7 and A549 human cancer cell lines with IC50 values 10.73, 13.61, 11.93, and 16.54 μM...
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Posted by admin on Mar 30, 2015 in |
This study investigated the antidiabetic activity of the aqueous extract of the leaves of Ageratum conyzoides. Streptozotocin induced hyperglycemia male adult albino rats were used to study the antidiabetic effect of Ageratum conyzoides aqueous extract (at doses of 100, 200 and 300 mg/kg respectively). The diabetic rats were housed in metabolic cages, for the whole period of the experiment. Body weight was evaluated at the beginning of the experiment and on days 7, 14, and 21 afterward. Water intake, food intake and urine excretion volume were also estimated. At the end of the experiment the rats were sacrificed and serum insulin concentration, total serum proteins and hepatic glycogen content were estimated. Lipid profile (total cholesterol (TC), High density lipoprotein (HDL)-cholesterol, low density lipoprotein (LDL)-cholesterol and triglycerides (TG)) were also assessed. The results showed significant effect of stimulating body weight growth at the doses of 200, 300 mg/kg. The effect of the extract on the food, water intake and water excretion showed a significant decrease in the 200 and 300...
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