Posted by admin on Jan 31, 2015 in |
Two spectrophotometric methods are presented for the simultaneous estimation of Zaltoprofen (ZLT) and Paracetamol (PAR) in laboratory prepared mixture and pharmaceutical dosage form without prior separation. Method (1) is simultaneous equation method (Vierodt’s method); which depends on the fact that absorbance of a mixture is the sum of the individual absorbance of the components. Method (2) is Q-Absorbance ratio method, which depends upon the fact that, the ratio of absorbance at any two wavelengths is a constant value independent of concentration or path length. Absorbances are measured at two wavelengths one being the λ max of ZLT (227.5 nm) and other being a wavelength of equal absorptivity of the two components (236 nm). Calibration curve of the two methods are linear over the concentration ranges of 4- 9μg/ml and 8-13μg/ml for PAR (247.5nm) and ZLT respectively. The two methods proved to be simple specific, accurate and precise. Solvent used is HPLC grade methanol. The two methods are validated as per ICH guidelines were accuracy, precision, repeatability and robustness are...
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Posted by admin on Jan 31, 2015 in |
A reversed phase high performance liquid chromatography coupled with diode array detector (HPLC–DAD) was developed and validated to quantitate diosgenin in berries of Solanum nigrum using C18 analytical column as stationary phase and acetonitrile: water (92: 08, v/v) as mobile phase. The flow rate of mobile phase was kept at 1.0 ml/min. Column oven temperature was set at 25 0C. The method was found to be linear over the range 1.0-60 µg/ml. The limit of detection and limit of quantitation of the developed method were found to be 0.33μg/ml and 1.0 μg/ml, respectively. The recoveries for the spiked samples ranged 96.85 – 103.68 %. The RSD values for intraday and interday precision studies of the method were all less than 1.11 %. The proposed method was found to be robust in terms of % w/w of diosgenin. The developed and validated method was applied for quantitative determination of diosgenin in extracts and formulations containing Solanum...
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Posted by admin on Jan 31, 2015 in |
Objectives: The aim of this study was to analyze and evaluate the practitioners’ prescribing habits of antibiotics in three cities of Bangladesh. Methods: A cross sectional health survey with manual data collection was carried out with a standardized questionnaire over three month period at the three cities Rajshahi, Jessore and Comilla in Bangladesh. The collected data was subjected to simple descriptive analyses including frequency distribution, mean and finally expressed in percentage. Results: A total of 900 prescriptions of outpatients were analyzed during the study period. It was found that the prescriber prescribed antibiotics to the patients who were suffering mainly from cold and fever, infections, diarrhea and gonorrhea. The highest prescribed antibiotic groups were cephalosporins (25.55%, 95%CI: 24.98-26.12), quinolones (23.75 %, 95%CI: 23.31-24.25), penicillins (15.78%, 95%CI: 15.41-16.15), macrolides (13.55%, 95%CI: 12.98-14.12) and metronidazoles (8.33%, 95%CI: 7.82-8.84) respectively. Two or more antibiotics were prescribed in 24.78 % of prescriptions. A total of 85% prescriptions had complete information on dosage form, 71.4% had complete direction for antibiotics use, 64% prescriptions had...
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Posted by admin on Jan 31, 2015 in |
Objective: The aim of this study was to evaluate the sleep quality and to identify risk factors for poor sleep quality in hemodialysis population. An additional aim of the study was to identify number of patients treated for their sleep problems. Method: 126 patients undergoing hemodialysis treatment were enrolled into the study. Subjective sleep quality was measured using Pittsburgh Sleep Quality Index. All the participants also completed Beck Depression Inventory questionnaire for evaluation of depressive symptoms. The pharmacotherapy and clinical, demographic and socioeconomic variables were also investigated. Results: Among the enrolled patients, poor sleep quality was reported in 67% (n=85). Depressive symptoms were identified in 75% (n=62) of the poor sleepers. Significant association was observed between variables like being single, female gender, older age, low socioeconomic status, increased financial reliability, spending more than INR 10,000 ($ 166) per month for treatment, overweight, morning dialysis shift and dialysis duration more than 3 years. Clinical conditions like depression, diabetes, limb pain, fatigue, hypertension, hypoalbuminemia, hypocalcaemia and hypophosphatemia and use of medications...
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Posted by admin on Jan 31, 2015 in |
The present study investigated the phytochemical analysis of the major bioactive constituents of medicinal important plant of Punica granatum peels in its aqueous extract and green synthesis of silver nanoparticles and their evaluation for antibacterial activity. Flavonoid, phenol & tannins, carbohydrates, glycosides and etc were highly concentrated in the peels extract. The synthesis and characterization of silver nanoparticles was confirmed by UV-Visible spectroscopy. UV-Visible spectroscopy of the reaction medium containing silver nanoparticles show the maximum absorbance peaks 430nm (1%), 373nm (3%), 379nm (5%). Green synthesis of silver nanoparticles analyzed against bacterial cultures by using the disc diffusion method. The silver nanoparticles have shown bactericidal effects against E.coli (MTCC-40), Staphylococcus aureus (MTCC-7443), Proteus vulgaris (MTCC-*1771). The peels extract of Punica granatum quikely reduces Ag+ to Ago and enhance synthesis of silver nanoparticles with highly antibacterial...
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