Posted by admin on Jan 31, 2015 in |
The humans head lice are a nuisance for millions of people worldwide with high prevalence in children. Head lice have been treated by methods that include the physical removal of lice, various domestic treatments and conventional insecticides. None of these methods render complete protection and there is clear evidence for the evolution of resistance and cross-resistance to conventional insecticides. Non-toxic alternative options are hence needed for head lice treatment or prevention and natural products from plants, especially essential oils (EOs) are good for safer control agents that may provide good anti-lice activity and low levels of evolved resistance. A few Essential oils have been tested as repellents with promissory results, although often in vitro tests and clinical trials produce contradictory results. The use of pyrethroids to control head louse infestations have suffered considerable loss of efficacy due to the development of resistance. In the last past few years, several new alternative products to synthetic pyrethroids have been developed and are sold in the market against head lice. The present...
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Posted by admin on Jan 31, 2015 in |
The present investigation is concerned with synthesis of new substituted benzoxazolinone derivatives (1-18) with the objective of discovering novel and potent antimicrobial agents. The structure of all the synthesized compounds were elucidated by elemental (C, H, N) and spectral (IR, 1HNMR and mass) analysis. The purity of the synthesized compounds was checked by thin layer chromatography (TLC). The obtained compounds were screened for their antibacterial as well as antifungal activities and compared with reference drugs ciprofloxacin and fluconazole respectively. All the newly synthesized compounds were screened for their antibacterial activity against S. aureus, E. coli, K. pneumoniae and antifungal activity against A. niger, C. albicans. Compounds 13 and 18 were found to be the most potent members of the present series; they showed maxium antibacterial and antifungal properties much better than the standard drug. The compounds 15, 16 and 17 exhibited good antibacterial activity as well as while antifungal...
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Posted by admin on Jan 31, 2015 in |
The urease (E.C.3.5.1.5) catalyzes the hydrolysis of urea to ammonia and is commonly used in clinical laboratories for the estimation of urea in biological fluids. The urease enzyme of sword bean (Canavalia gladiata) has been purified in single-step by affinity chromatography using epoxy activated agarose containing urea as the affinity ligand. The yield of the purified enzyme was about 83% with specific activity of about 495 U/mg of protein with 14-fold purification. The final preparation had a transparent appearance with free ammonia content less than 0.01 µg/unit and was stable for 15 months at 4-8°C. The enzyme acted optimally at pH 7.5 and 25°C. Thermal stability studies indicated that at pH 7.5 no loss of enzyme activities were recorded up to 35°C for 30 mins. SDS−polyacrylamide gel electrophoretic analysis showed that the purified enzyme was apparently homogeneous and had a molecular weight of approximately 87 kDa. Single band was observed in both native and...
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Posted by admin on Jan 31, 2015 in |
The purpose of this study was to evaluate antimicrobial activity of Bursera penicillata leaf extract against human infected bacteria. Antimicrobial activity of Petrolium ether, Methanolic and Aqueous extracts from leaves of Bursera penicillata were tested against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus saprophyticus, Enterococcus faecalis and Enterobacter cloacae using Agar well diffusion method. Solvent extracts from the leaves of the plant shown a considerable antimicrobial activity against most tested microorganisms. The most active extract was Methanolic extract from the leaf against Klebsiella pneumoniae and significantly inhibited the bacterial activity. Minimum inhibitory concentration for aqueous and methanolic extracts of leaves ranged from 5.0–20.0 mg/ml and ethyl acetate and petroleum ether leaf extracts ranged from 25.0µg-55.0 µg and 45.0-85 µg/ml. It further reflects a hope for the development of many more novel chemotherapeutic agents or templates from such plants which in future may serve for the production of synthetically improved therapeutic...
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Posted by admin on Jan 31, 2015 in |
Solubility is the important parameter to design a dosage form; it is very difficult to formulate an insoluble drug in to final formulation. In This present study deals with the enhancement of solubility of poorly water soluble drug Meloxicam.: The liquisolid system is a novel technique for solubility enhancement and dissolution improvement of low soluble drugs. Liquisolid technique (powdered solution technology) is used to enhance the solubility parameter and dissolution rate of the drug. liquisolids were prepared by using non volatile solvents or hydrophobic carriers (like PEG 400, Tween 80, span 20) and their effect on dissolution of Meloxicam was studied. By using these solvents individually or in combination and find out the optimized formulation with good solubility.Based on the solubility studies the polyethylene glycol 400 (PEG400) is chosen as co solvent, Microcrystalline cellulose (MCC) used as carrier material and Aerosil used as coating material. In liquisolid system different nonvolatile or hydrophobic solvents are continuously added to the liquid until it convert in to a free flowing solid system...
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