Posted by admin on Dec 25, 2014 in |
Column chromatography of ethyl acetate of Bauhinia retusa afforded a novel flavonoid identified as [ rhamnetin-3-O-α- L-rhamnopyranosyl-3’-O-(prop-1-enyl)], in addition to kaempferol, isorhamnetin-3-α-rhamnoside, qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside, and quercetin 3,7-di-o- β-D-glucoside have been isolated for the first time. Their structures were established from extensive spectroscopic techniques (UV, MS, 1H and 13C-NMR), chemical studies in addition to comparison with literature data and /or authentic samples. More over,qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside and ethyl acetate fraction exerted pronounced antioxidant activities (SC50 µg/ml ,13.5 , 14.7 and 18.7 ) respectively, compared with vitamin C (SC50,,13.9 µg/ml ) by DPPH radical scavenging...
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Posted by admin on Dec 25, 2014 in |
Natural medicines and nutraceuticals are good sources of anti-oxidant and are gaining popularity for their capacity to enhance, health-protective effects. The aim of the present study was to investigate the anti-oxidant activity of some natural medicine from the Himalayan region of Nepal. Carefully collected plant materials were subjected to maceration with methanol for about 48 hours. Anti-oxidant activity of methanolic extracts was assessed using DPPH as a free radical in different concentrations (0. 1 µg/ml, 1 µg/ml, 10µg/ml, 100µg/ml). Percent inhibition and effective Concentration (EC50) values were calculated. EC50 of Acacia catechu (0.89µg/ml), Cissus repens (3.44µg/ml) Psidium guajava (3.65µg/ml) were calculated and found to be more effective than ascorbic acid (4.48µg/ml). Drynaria propinque (5.21µg/ml) Eskemukerjea megacarpum (5.24µg/ml) and Nelumbo nucifera (6.76µg/ml) were comparable to standard anti-oxidants whereas Ficus relifiosa, Tinospora sinensis, Elaeocarpus sphaericus, and Cannavis sativa showed poor free radical-scavenging activity. Our results revealed that Acacia catechu (resin) showed greater anti-oxidant activity than other extracts and standard, suggesting that it could be the better anti-oxidant and diseases preventive remedies...
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Posted by admin on Dec 25, 2014 in |
Mycobacterium tuberculosis is the causal agent of tuberculosis which is an infectious disease responsible for more than 2 million deaths per annum worldwide, mainly in developing countries. Cell division of this microbe is very crucial for propagation. Filamenting temperature sensitive mutant Z (FtsZ) is very important player that seems to assemble into a dynamic ring (Z- ring) on the inner surface of the cytoplasmic membrane which involved in stabilizing Z-rings at the place where the division occurs and the formation of the ring is the signal for septation to begin. To control this disease researchers have investigated many naturally occurring bioactive molecules from different medicinal plants. In the present docking study, we screened a total of fifty-one different bioactive molecules found in three medicinal plants namely Justicia adhatoda, Abrus precatoriu sand Dracaena angustifolia to determine the inhibition against M. tuberculosis FtsZ cell divisional protein. No inhibitory effect was observed for the molecule found in D. angustifolia on the other hand, three compounds namely abrectorin, precatorine and gallic acid of...
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Posted by admin on Dec 25, 2014 in |
Compounds with a 2(H)-1, 4-benzoxazin-3(4H)-one skeleton have attached the attention of phytochemistry researchers since 2, 4-dihydroxy-(2H)-1,4-benzoxazin-3(4H) and 2,4-dihydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one were isolated from plants belonging to the Poaceae family. These compounds exihibit interesting properties11 such as phytotoxic, antimicrobial12, antifeedent, antifungal and insecticidal properties. The reaction of CSI with 2-Chlorobenzyl alcohol result in N-chlorosulphonyl derivatives, which show antibacterial potency. Futher, N-chlorosulphonyl derivatives can be easily cyclofunctionalized. This cyclofunctionalization has been exploited in the synthesis of the title compounds (3). Thus, the title compound being oxygen analogues of 1, 3-thiazine derivatives together with N-sulphonamide moiety could be expected to exhibit enhanced...
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Posted by admin on Dec 25, 2014 in |
Lopinavir (LPV) is potent and poorly bioavailable molecule which is frequently administered protease inhibitors in the treatment of the HIV. Solid Lipid Nanoparticles (SLNs) of LPV were prepared using High Speed Homogenization. LPV were successfully encapsulated in the mixture of Glyceryl Behenate and Glyceryl monostearate. Poloxamer 188 was used as a surfactant for the preparation of the SLNs. Formulation composition and process parameter were optimized to get the desired quality of the SLNs. Formulation PF5 have mean particle size 185 ± 5 nm with Polydispersity index 0.11 ± 0.01 which indicates very narrow particle size distribution. % Entrapment efficiency was 86.2 ± 1.5. Slow drug release profile indicates the homogeneous dispersion of LPV in lipid matrix. SLNs have spherical shape and smooth surface which has been confirmed using SEM analysis. XRPD spectra show the reduction in crystalline behaviour of the drug and the lipid after formation of the SLNs. There was no significant change in the mean particle size and Polydispersity index after 6 month storage at 25°C/60%...
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