Posted by admin on Feb 26, 2015 in |
To develop and validate simple and precise methods RP – HPLC (Method A) and Dual wavelength method (Method B) for simultaneous estimation of Simvastatin (SIM) and Sitagliptin (SITA) in combined dosage form. Methods used are Method A, the chromatographic separation was achieved by using mobile phase acetonitrile: methanol: water (50:30:20), a C18 column. The mobile phase was pumped at a flow rate 1 ml /min and the eluents were monitored at 250 nm. Method B, two wavelengths were selected for each drug in such a way that the difference in absorbance is zero for the second drug. At wavelengths 225 and 248 nm SITA has equal absorbance values, therefore, these two wavelengths have been used to determine SIM, on similar basis 254 and 274 nm were selected to determine SITA in the combined...
Read More
Posted by admin on Feb 26, 2015 in |
Malathion is a widely used organophosphorous pesticide that a large number of populations are undesirably exposing themselves to severe health risk due to taking up the contaminated foods, water and vegetables containing malathion. The present study was carried out through histopathologic test to evaluate the extent of damage caused by malathion in the liver and kidney tissues of mice. Twenty five adult female albino mice were used and dispense mainly into two groups as control group and treatment group. The mice of control group were feed with poultry food without any ingredient of malathion. Each of the treatment groups (group1, group 2, group 3 and group 4, containing five animals in each group) were supplied everyday with the same type of food containing the following amounts of malathion as 65mg, 13mg, 2.6mg, and 0.52mg per 50gm (10gm food/mouse) of food, respectively. After fifteen days the animals of both groups were sacrificed and the organs of sacrificed animal were subjected to histopathological examination and then visualized under light microscope. Results...
Read More
Posted by admin on Feb 26, 2015 in |
Metal oxide in the nanorange acquires distinctive properties that depend on size, chemical composition and surface chemistry. Among the metal oxide nanoparticles, the zinc oxide nanoparticles (ZnO NPs) are commonly used for their antimicrobial properties. ZnO nanomaterials are incorporated into a variety of skin coatings because of their antimicrobial and/or antifungal properties. Most of the future therapeutic applications of NPs are based on intravenous/oral administration. Experiments on their interaction with blood components especially erythrocytes are of immense importance, if the nanoparticles are to be administered intravenously. Haemolytic potential of ZnO NPs was assessed spectrophotometerically as well as by phase contrast microscopy. In our study, interaction of different concentrations of ZnO NPs with erythrocytes revealed absence of hemolysis by spectrophotometric method. On the other hand, phase contrast microscopic examination revealed concentration dependent clustering of erythrocytes. The detailed investigation about interaction of erythrocytes with ZnO NPs is needed before their clinical...
Read More
Posted by admin on Feb 26, 2015 in |
Optimization of total carotenoids production is dependent on various cultural and physical parameters for pigmented yeasts. The carotenoid production of the red yeast Rhodotorula mucilaginosa MTCC11835 was optimized using response surface methodology. The carotenogenesis in yeasts is known to be affected by different nutritional factors and environmental stress factors. Effect of various growth parameters like carbon source (glucose; glucose:glycerol (1:2); maltose), nitrogen source (peptone; casein hydrolysate; yeast extract), pH (3.5;5.0; 6.5) and stress factors (Tween20; hydrogen peroxide) were studied for the maximum yield of carotenoids. The yeast was shown to produce high amount of total carotenoids (21.77 mg g-1) in a medium containing glucose: glycerol (1:2), peptone, 0.5% Tween 20, 2.5 mM H2O2 and pH...
Read More
Posted by admin on Feb 26, 2015 in |
Different prokineticin receptor antagonists, based on the triazinedione scaffold, were synthesized by a new efficient method. Here we demonstrated that 5-benzyltriazinediones substituted in position para of the benzyl group with halogens provide compounds endowed with interesting selectivity for the Prokineticin receptor 1 (PKR1). BRET technology indicates that such substitution results in increased affinity for the PKR1. The affinity for PKR2, always in µM range, was never significantly affected by the para-halogen-benzyl pharmacophores. The analog bearing a para-bromobenzyl pharmacophore (PC-25) displayed the highest affinity for PKR1 (~18 times higher than the reference PC-1 that bears apara-ethyl benzyl group) and the highest selectivity (~300 times). The other halogen substituted analogs (PC-7, PC-18 and PC-35), showed selectivity for PKR1 more than 100 times higher than for PKR2. Using transgenic mice lacking one of the two PKRs we demonstrated that all these compounds were able to abolish the Bv8-induced hyperalgesia in mice still expressing the PKR1 at doses lower than those necessary to abolish hyperalgesia in mice expressing only the PKR2. The dose...
Read More
