Posted by admin on Dec 25, 2014 in |
The biological synthesis of magnetite nano-bio hybrid using plant extracts plays an important role in the field of nanotechnology. The present study demonstrates bio-reductive synthesis of protein coated magnetite (Fe3O4) nano-bio hybrid using leaf extracts of Datura inoxia plants and its encapsulation into liposome. Liposome is vesicles of varying size consisting of a spherical lipid bilayer and an aqueous inner compartment that are generated in vitro. Magnet-driven liposome used for the targeted delivery of drugs to organs and tissues. These liposome preparations contain encapsulated drug components and finely dispersed magnetic particles inside the liposome. Liposome is useful in terms of biocompatibility, biodegradability, low toxicity and can control bio distribution by changing the size, lipid composition, and physical characteristics. Furthermore, liposome can entrap both hydrophobic and hydrophilic drugs and are able to continuously release the entrapped substrate, thus being useful drug carriers. The nano bio hybrid based magneto liposome has been prepared by reversed phase evaporation (REV) method using oleic and linoleic acid which has been shown to be driven...
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Posted by admin on Dec 25, 2014 in |
The Present Study was planned to assess the acute toxicity of Solanum lycopersicum Leaf. The study was designed according to the OECD guidelines as per that the dose level used as 5, 50, 300, 2000, 4000 mg/kg. Mice were orally administrated single dose of 200 and 400 mg/kg of ethanolic extract of Solanum lycopersicum. Physical, Biochemical, Hematological as well as histopathological parameters were unaltered throughout the study. Mortality, signs of toxicity, bodyweight, food consumption and gross findings were observed for 14 days post treatment of Solanum lycopersicum extract. In addition, no significant differences were noticed in the body and organ weights between the control and treated groups. These results state that aqueous extract of Solanum lycopersicum is toxicologically safe by oral...
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Posted by admin on Dec 25, 2014 in |
Polyunsaturated Fatty Acids (PUFA) have been shown to be of major importance in the prevention and treatment of a range of human diseases and disorders. A number of microorganisms have been shown to produce and accumulate PUFA. In the current study we have isolated 86 lipid producing microbes from various sources like a river from northern India, lakes from southern India and marine environments off the western coast of India. The isolates were shortlisted following a preliminary screening scheme by studying a set of parameters like intensity of lipid accumulation as judged by Sudan Black B staining, amount of biomass on nitrogen limited semi-synthetic medium, lipid quantity per gram biomass, relative quantities of saturated and unsaturated fatty acids and reproducibility of results. Lipid sample was extracted and derivatized for GC analysis using standard procedure. Further, GC/MS analysis confirmed that 5 isolates produce PUFA of omega-6(LA) and omega-3(ALA, ETA) type. Selected cultures have been identified by 16S r-RNA sequencing and are being optimized for PUFA production by the technique of...
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Posted by admin on Dec 25, 2014 in |
Morinda Pubescens belonging to the family rubiaceae has been reported to posses many medicinal properties according to indigenous system of medicine. The search for biologically active compounds from natural sources has always been of great interest to researchers looking for new drugs useful in infectious disease. In the present study, the various extracts of Morinda Pubescens bark was tested for their antibacterial and antifungal activities against gram positive bacteria such as Staphylococcus aureus, Bacillus cereus, gram negative organisms such as Escherichia coli, Pseudomonas aeruginosa and fungi such as Candida albicans, Aspergillus niger respectively by cup plate method. All the extracts were effective against the tested microorganism which was comparable with standard antibiotics. The ethanolic extract showed highest zone of inhibition then other extracts tested. The zone of inhibition of the extracts of Morinda Pubescens indicating that the plant of Morinda Pubescens can fight these microorganism effectively due to the presence of phenol,triterpinoids and tannins it could be better alternative to the modern...
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Posted by admin on Dec 25, 2014 in |
Multiparticulate drug delivery system are mainly oral dosage forms consisting of a multiplicity of small discrete units, each exhibiting some amount desired characteristics such as pellets.The purpose of present study was to prepare sustained release robust matrix pellets of Glipizide. The study revealed successful application of factorial design, optimization of extrusion spheronization process, and sustaining release of Glipizide for formulating uniform, spherical Glipizide pellets. Speed and time of spheronization is critical parameter for optimum sphericity. The Optimum speed and time of operating spheronization is 1350 rpm and 15 min. The results of this study showed that combination of HPMC K100M LVCR as hydrophilic matrix polymer and PEG 400 as potential plasticizer is effective and useful for sustaining the Glipizide release to treat diabetes mellitus. The in-vitro studies showed Q16 at approximately 80% cumulative release in case of F4, F5, F6, It indicates that this system can sustain the release upto 24 h which is desirable for sustained release specificity. Although, the hydrophilic matrix polymer optimization is critical for drug...
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