Posted by admin on Feb 25, 2015 in |
Environmental pollution is one of the undesirable side effects of industrialization and found responsible factor for environmental degradation. Water samples and crabs Barytelphusa cunicularis were collected from Gadhinglaj tahsil and analyzed for Physico- chemical parameters and heavy metals respectively. The results showed high range of pH and high values of alkalinity, hardness, chloride, sulphate, TDS, COD. The heavy, metal contamination was in the order of sodium > iron >potassium. The results indicate altered physico-chemical parameters and heavy metals resulting in massive destruction of gill architecture which was concentration and time dependent. Fluctuations in physico-chemical parameters has influenced gill lammelles of crab Barytelphusa cunicularis. Histopathological studies revealed noticeable changes in the gills like clumping of cells, detachment of epithelial surface in primary lamella and destruction of mucosal cells. The gills showed vacuolization in the gill stem, ruptured gill lamellae, connective tissue cells was damaged, destructed with congestion of haemocytes in the gill lamellae are observed. The results obtained were discussed in relation to physico-chemical parameter over respiratory mechanism in crab...
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Posted by admin on Feb 25, 2015 in |
In this pilot study, Zarrouk medium was optimized in terms of biomass production and metabolites for the culture growth of, Spirulina species; Spirulina maxima and Spirulina platensis and their extract were tested against a nosocomial fungal species Candida albicans (C. albicans) to explore anticandidal activity. The corresponding growth and metabolite were measured in terms of turbidity, chlorophyll, carotenoids and protein contents. The culture was harvested by centrifugation and extracted with 75% methanol by freeze thaw method. Supernatant was collected, evaporated to dryness and stored at -20 0C. Anticandidal activities were assessed based on the agar-well diffusion method. The lawn of C. albicans was maintained at 1.5×105 CFU/ml on Sarbourd dextrose (SD) agar plates under sterile conditions. The plates were dried at 37 0C for 30 min. Wells of 6_mm diameter were created by using sterile agar borer. The dried supernatants were dissoluted in normal saline (0.8% NaCl) and poured in each well (100µl); Control well carried 100µl normal saline. Poured wells were incubated for 18 h at 37 0C...
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Posted by admin on Feb 25, 2015 in |
A new simple stability indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous estimation of Paracetamol, Tramadol and Dicyclomine in combined pharmaceutical dosage form. An Agilent Zorbax SB C18 (250mmx4.6mm, 5microns) column with mobile phase containing ammonium acetate: acetonitrile (700:300 v/v) was used. The flow rate was maintained at 1.0 mL/min, column temperature was 30°C and effluents were monitored by using a photodiode array detector at 233 nm. The retention times of Paracetamol, Tramadol and Dicyclomine were found to be 2.331, 3.182 and 7.650 min, respectively. Correlation co-efficient for Paracetamol, Tramadol and Dicyclomine were found to be 0.99, 0.99 and 0.99 respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Paracetamol, Tramadol and Dicyclomine in formulations was found to be in a range of 98-102%, 98-102% and 98-102% respectively. Paracetamol, Tramadol and Dicyclomine dosage form is also subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved and...
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Posted by admin on Feb 25, 2015 in |
An Accurate, sensitive, precise and stability indicating high performance thin layer chromatographic method has been developed and validated for the estimation of Glimepiride and Metformin hydrochloride. The method was developed using TLC aluminium plates precoated with silica gel 60F254 as the stationary phase and 0.5% Ammonium Sulfate: Methanol (7.5:2.5 v/v) as mobile phase. Densitometric analysis of Glimepiride and Metformin hydrochloride was carried out in the absorbance mode at 228 nm. The two drugs were satisfactorily resolved with RF values 0.73 and 0.45 for Glimepiride and Metformin hydrochloride respectively. Linearity was found to be 600-2100 ng/band for Glimepiride and 200-700 ng/band for Metformin hydrochloride. The method was found to be accurate, precise, and robust according to acceptance criteria. The limit of detection (LOD) was found to be 0.05 ng/band and 0.32 ng/band for Glimepiride and Metformin hydrochloride respectively. The limit of quantification (LOQ) was found to be 0.16 ng/band and 0.96 ng/band for Glimepiride and Metformin hydrochloride respectively. The proposed HPTLC method was successfully applied for the forced degradation study...
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Posted by admin on Feb 25, 2015 in |
Lannea coromandelica (Anacardiaceae) is a tropical tree commonly called as Indian ash tree. Its various parts is said to possess medicinal property and used in Ayurveda and other ancient system of medicine for the treatment of various ailments including treatment of inflammation, gout, cholera, leprosy, vaginal troubles etc. Its Pharmacological study revealed anti-inflammatory, antimicrobial, hypotensive, anticancerous activities. Leaves of Lannea coromandelica were collected from Azhagar malai, Madurai. The sample specimen of Lannea coromandelica was identified and authenticated by Dr. Jaya Raman, Taxonomist, Chennai. The present work was carried out to identify some of the phytocomponents present in the ethanolic leaf extract of the L. coromandelica by GC-MS technique, to ascertain the medicinal properties of the plant and also to assess its anti-oxidant and anti-arthritic potential of ethanolic leaf extract of Lannea coromandelica. The results revealed that ethanolic extract is mainly composed of oxygenated hydrocarbons and predominantly phenolic hydrocarbons. These different active phytochemicals may be responsible for wide range of activities, which may help in the protection against incurable diseases. The future in vivo investigation...
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