Posted by admin on Apr 30, 2015 in |
The objective of the present work is to develop simple, precise and accurate colorimetric methods for the estimation of aripiprazole (ARP), tapentadol (TAP) using chloranilic acid reagent. ARP belongs to the class of antipsychotics. TAP is centrally acting analgesic drug. The methods are based mainly on charge transfer complexation of these drugs with p-chloranilic acid to give magenta purple colored products which were extracted into chloroform. The products were quantified at 543 nm for both ARP and TAP. The linearity of the methods was assessed in the range of 80-400 µg/ml for ARP and 200-1000µg/ml for TAP, respectively. The LOD and LOQ are 5.17 and 15.66; 82.5 and 250 for ARP and TAP, respectively. The colorimetric methods were extensively validated as per ICH guidelines and all the parameters were within the acceptance criteria with a correlation of 0.9999 and the % RSD less than 2. The results of the accuracy studies were nearer to 100%. The methods were proven to be more accurate, simple, precise and rapid by statistical...
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Posted by admin on Apr 30, 2015 in |
Objective: The present study was an effort to evaluate the effect of Ocimum sanctum on plasma concentration of metformin HCl and investigating its pharmacokinetic parameters using bioanalytical method. Methods: Seven groups comprising of six rats were used. Group I served as normal control and Group II was negative control of Streptozotocin induced Diabetic rats. Group III received 100mg/kg p.o. Metformin alone while Group IV and V were administered with 100mg/kg and 150mg/kg p.o. extract respectively. Group VI and VII were given metformin and extract in combination. Blood glucose level was determined in treated rats for 0.5, 2, 4, 8, 12 and 24hr as part of protocol I and directly on 2nd, 4th and 7th day as part of protocol II. RP-HPLC method was developed and validated for estimation of plasma levels of metformin HCl in plasma. With the help of above developed method, pharmacokinetic parameters were evaluated in plasma for metformin alone and combination with Ocimum sanctum. Results: RP-HPLC method was developed successfully for determination of plasma concentration of...
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Posted by admin on Apr 30, 2015 in |
Diabetes is a non communicable chronic metabolic disease characterized by high blood glucose level in the body. Targeting alpha amylase to control diabetes has long been studied. This study focused on developing pharmacophore model for set of inhibitor that possess anti amylase activity based on previous studies. Developing a pharmacophore model for these compound yields a hypothesis of ADDRR. Furthermore 3D QSAR model is carried out with good correlation activity for training set as .98 and test set as .92. Database screening for the developed model list out top compounds. High throughput virtual screening is performed for this top compound set. Two ligands quercetin 3-glucosyl rhamnosyl galactoside and rhodionin is found to have good binding affinity over alpha...
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Posted by admin on Apr 30, 2015 in |
The experiment was performed at the Department of Pharmacology in collaboration with the animal house Microbiology Department, Bangladesh Agricultural University, Mymensingh during the period from Jan 2005 to May 2005. To complete the research work following steps were followed.The aim of this study was to assess the possible comparative efficacy of Turmeric (Halud), Garlic (Rashun) and Aloe (Ghrita kumari) with Antibiotic Nebanol (Neomycin Sulphate and Bacitracin Zinc) on artificially induced wound in 2.5 cm length and 0.5 cm depth were made on the two thigh muscles an experimental model. Twenty five guinea pigs of both sexes age ranged between 6 to 8 months were divided into five groups (each group containing five animals). The first, second and third groups received herbal medicine e.g.; Turmeric paste, Garlic paste, Aloe gel and fourth group received Antibiotic Nebanol powder (Neomycin Sulphate and Bacitracin Zinc) respectively. The fifth/control groups were observed without medicine. Turmeric, Garlic, Aloe & Nebanol treatment reduced the wound within 12, 15, 17 & 14 days respectively. On the other...
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Posted by admin on Apr 30, 2015 in |
Tithonia diversifolia was collected from 27 localities of Côte d’Ivoire. The oils of the leaves and stems obtained by hydrodistillation using Clevenger-type apparatus have been studied by GC and GC-MS. The identified components accounted for 91.4% – 96.9% of leaves and 93% – 95.6% of stems. The content of the main components varied from sample to sample. α-pinene (0.9 – 47.4%), limonene (0.5 – 65.5%), (Z)-β-ocimene (0.0 – 35.9%) and 3-methyl-2 (2-methylbuthenyl) furan (0 – 94%) were noted as major components of the oil from the leaves. Whereas, the stem oil was rich in α-pinene (5.8 – 89.8%), sabinene (0. – 5.2%), β-pinene (0.3 – 10.5%), limonene (1.3 – 36.5%), (Z)-β-ocimene (0 – 39.3%), thymol (0 – 4%) and spathulenol (0.1 – 11.8%). Due to the chemical polymorphisme, the results of oil obtained from leaves and stems were submitted to hierarchical cluster, principal components analysis and discriminant factoriel analysis which allowed the distinction of two groups within the oil samples of each organ. According to the essential oil from...
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