Posted by admin on Feb 28, 2015 in |
In-vitro antibacterial and antifungal activity of Tridax procumbens plant parts (leaf and stem) were investigated by agar well diffusion method. The petroleum ether, methanol and aqueous extracts of the plant parts were tested against two bacterial strains i.e., Bacillus subtilis and Escherichia coli and two fungal strains i.e., Trichoderma reesei and Fusarium oxysporium. The results of antibacterial activity showed that methanol extract was effective and aqueous extract was devoid of any significant activity. Methanol extract of Tridax procumbens showed maximum antifungal activity. The results lend credence to the folkloric use of this plant in treating microbial infection and showed that Tridax procumbens leaves and stem could be exploited for new potent antimicrobial...
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Posted by admin on Feb 26, 2015 in |
A simple, rapid, sensitive, precise, accurate and economic isocratic stability indicating RP-HPLC chromatographic method was developed and validated for the simultaneous estimation of Glimepiride and Ezetimibe in bulk and tablet dosage form. The method was developed through a Hypersil ODS C18 (150mm x 4.6 mm, 5m) column, mobile phase comprised of phosphate in water as buffer PH adjusted to 4.8 with tri ethylamine, acetonitrile in proportion ratio 30:70v/v and at the flow rate of 1 ml/min. GLM and EZE were eluted at acceptable retention times of 3.328 and 2.322 minutes respectively with good resolution by monitoring UV detection at 237 nm. Calibration plots were linear in the concentration range of 2.5-15 µg/ml for GLM and 25-150 μg/ml for EZE with correlation coefficient(r2) 0.999 and 0.999 respectively. The total run time is 6 min. The studies were carried out by conducting deliberate degradation of the sample with exposure to stress conditions like acidic (1M HCl), alkaline (1M NaOH), 1050C heat, oxidizing agents (H2O2) and water. This method was validated and...
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Posted by admin on Feb 26, 2015 in |
A series of novel 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl-amino)-4-(substituted phenyl)azetidin-2-one analogues (3a-e) were synthesized starting from 2-thiouracil. Reaction of 2-thiouracil with 4-fluorobenzylchloride gave 2-(4-fluorobenzylthio) pyrimidin-4(3H)-one which on chlorination with POCl3 yielded 2-(4-fluorobenzylthio)-4-chloropyrimidine. Further reaction of compound 2-(4-fluorobenzylthio)-4-chloropyrimidine with hydrazine hydrate generated the key intermediate 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl)hydrazine (1). Reaction of compound 1 with appropriate substituted aromatic aldehydes furnished 2-(substituted benzylidene)-1-(2-(4-fluorobenzylthio) pyrimidin-4-yl)hydrazine (2a-e), which on further cyclisation with acetyl chloride in dry benzene furnished 1-(2-(4-fluorobenzylthio)pyrimidin-4-yl-amino)-4-(substituted phenyl) azetidin-2-one analogues (3a-e). Structural assignments of the synthesized compounds were based on their IR, 1H NMR, Mass and analytical data. All the synthesized compounds 3a-e were screened for their preliminary antimicrobial properties. Some of the compounds exhibited promising antimicrobial...
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Posted by admin on Feb 26, 2015 in |
Antihypertensive properties of plant can be evaluated by in vitro method on inhibition of Angiotensin Converting Enzyme (ACE) activity. In this research, we investigate the inhibitory effect of several common edible plants on blocking ACE activity. ACE activity was evaluated by using N-hippuryl-L-histidyl-L-leucine (HHL) as substrate and the inhibitory effect of extracts were determined based on the level of hippuric acid by measuring its absorbance using spectrophotometer. Gelatin-salt block test for detection of tannins was carried out prior to the enzymatic assay. Among the extracts tested, Peperomia pellucida L. showed strong inhibitory activity with IC50 value of 7.17μg/mL, followed by Nasturtium officinale and Sesamum indicum L. with IC50 values of 15.44 and 30.16μg/mL, respectively. Furthermore, Peperomia pellucida was the most potent inhibitor of ACE activity in vitro with IC50 value of 7.17 µg/ml, which was comparable to that of captopril (13.68 µg/ml), a potent ACE inhibitor. This study shows that these plants may develop as antihypertensive...
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Posted by admin on Feb 26, 2015 in |
Peptic ulcers is one of the major gastrointestinal disorder in human being that generally associated with the infection of Helicobacter pylori, a gram-negative microaerophilic bacterium in stomach. It is also linked to the development of the stomach cancer. The aim of this study was to investigate the in vivo properties of the sub-unit pili proteins with molecular weight of about 49,6 kDa in mice. The bacterium was firstly cultured on the plate of TSA-B (Trypticase Soy Agar with 5% Sheep Blood) to prepare the protein of interes using bacterial cutter and SDS-PAGE. The purified protein was used for a vaccine emulsified with commercial cholera toxin and give orally. The immunized mice showed a significant protection against challenge with live H. pylori cells. In contrast, animals that received the 49, 6 kDa protein without adjuvant as well as the negative control with PBS failed to inhibit adherence of the bacteria, as indicated by a severe damages of gastric tissues. This study has indicated that the sub-unit pili proteins trigered the...
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