Posted by admin on Jan 31, 2015 in |
Whole plant parts, fruits, roots, bark, peel, seeds and pulp of Carica papaya are known to have medicinal properties. It has been used for treatment of numerous diseases like warts, corns, sinuses, eczema, cutaneous tubercles, blood pressure, dyspepsia, constipation, amenorrhoea, general debility, expel thread worms and stimulate reproductive organs. Ayurvedic literature reveals that papaya leaf extract has haemostatic properties and recent studies revealed its ability on platelet augmentation in cyclophosphamide induced thrombocytopenia rat model. Pilot studies done in dengue patients with leaf juice revealed the effect of leaf juice on elevating white blood cells, platelet count and recovery without hospital admission. Hence, in the current study, an effort was taken to study the phytochemical profile of papaya leaf extract using Liquid Chromatography-Mass Spectroscopy (LCMS). Aqueous extract of young leaves were taken and subjected to LCMS analysis for phytochemical profiling using Water and acetonitrile as mobile phase. On LCMS analysis followed by integrated library search, 21constituents were identified and it included pharmacologically active phyto compounds, alkaloids, phenolics, flavonoids and also,...
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Posted by admin on Jan 31, 2015 in |
The present investigation deals with the determination of in vitro antioxidant activity as well as the phenols and ascorbic acid content in in vivo (leaf, stem, root) and in vitro (unorganised static callus cultures) plant parts of Pluchea lanceolata. Unorganised static callus cultures (eighteen months old) were grown on MS medium supplemented with NAA (1.0 mg/l) and BAP (0.5 mg/l) using leaf explants. Antioxidant activity was analysed in terms of DPPH radical scavenging assay, total phenolic contents were estimated by folin ciocalteau phenol reagent method and ascorbic acid (vitamin c) was analysed by 2, 4-dichlorophenol indo-phenol dye method. Antioxidant potential was found to be maximum (93.55 %) in leaf parts and minimum in stem (54.74 %). Lower IC50 value indicates high antioxidant activity which was maximum in stem (665.90±0.23) and minimum in leaf (123.37±0.16). Total phenols and ascorbic acid were also higher in in vivo tissues (leaf) as compared to in vitro tissues. In the present study excellent DPPH radical scavenging activity (RSA) was found in all extracts of...
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Posted by admin on Jan 31, 2015 in |
The aim of this investigation was to develop a solid microemulsion of Glimepiride for enhancing its solubility, and its dissolution rate. For this purpose, solubility of Glimepiride was determined in various vehicles. Oil, Surfactant and Cosurfactant were selected based on the solubility. Pseudo-ternary phase diagrams were constructed to identify the microemulsion existing zone. The optimized microemulsion formulation was characterized for its refractive index, % transmittance, pH, viscosity, drug content and particle size. Particle size of the optimized microemulsion formulation was found to be 38.83 nm. Various adsorbents were incorporated in the optimised liquid microemulsion to get solid microemulsion. The solid microemulsion from Aerosil 200 was optimized because of very low amount (1:1) of Aerosil was incorporated in microemulsion as compare to other adsorbent. The prepared solid microemulsions were subjected to characterisation for angle of repose, bulk density, tapped density, Hausner’s Ratio, Carr’s index, drug content, in-vitro drug release study and stability studies. The solid microemulsion with Aerosil 200 showed excellent free flowing property and % compresabilty. From in-vitro drug...
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Posted by admin on Jan 31, 2015 in |
The humans head lice are a nuisance for millions of people worldwide with high prevalence in children. Head lice have been treated by methods that include the physical removal of lice, various domestic treatments and conventional insecticides. None of these methods render complete protection and there is clear evidence for the evolution of resistance and cross-resistance to conventional insecticides. Non-toxic alternative options are hence needed for head lice treatment or prevention and natural products from plants, especially essential oils (EOs) are good for safer control agents that may provide good anti-lice activity and low levels of evolved resistance. A few Essential oils have been tested as repellents with promissory results, although often in vitro tests and clinical trials produce contradictory results. The use of pyrethroids to control head louse infestations have suffered considerable loss of efficacy due to the development of resistance. In the last past few years, several new alternative products to synthetic pyrethroids have been developed and are sold in the market against head lice. The present...
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Posted by admin on Jan 31, 2015 in |
The present investigation is concerned with synthesis of new substituted benzoxazolinone derivatives (1-18) with the objective of discovering novel and potent antimicrobial agents. The structure of all the synthesized compounds were elucidated by elemental (C, H, N) and spectral (IR, 1HNMR and mass) analysis. The purity of the synthesized compounds was checked by thin layer chromatography (TLC). The obtained compounds were screened for their antibacterial as well as antifungal activities and compared with reference drugs ciprofloxacin and fluconazole respectively. All the newly synthesized compounds were screened for their antibacterial activity against S. aureus, E. coli, K. pneumoniae and antifungal activity against A. niger, C. albicans. Compounds 13 and 18 were found to be the most potent members of the present series; they showed maxium antibacterial and antifungal properties much better than the standard drug. The compounds 15, 16 and 17 exhibited good antibacterial activity as well as while antifungal...
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