Posted by admin on Dec 1, 2014 in |
Chonemorpha fragrans (Moon) Alston is a liana belongs to family Apocynaceae. Due to its ethanobotanical importance, it is used intraditional medicinal systems as a part of various medicinal preparations. In the work presented here the plant has been explored for its antioxidant potential and DNA protection ability. The antioxidant potential of C. fragrans was determined using DPPH, FRAP, Phosphomolybdanu and H2O2 Scavenging Capacity. Phenol and flavonoid content was measured followed byCorrelation analysis between antioxidant activities andphenols and flavonoid content. In addition, DNA protection ability of extracts was evaluated using double stranded plasmid DNA. C. fragrans extracts have shown significant antioxidant potential. Phenol content showed high correlation with Phosphomolybdenum reduction potential where as flavonoid content with Phosphomolybdenum as well as H2O2 radical scavenging potential. All the extracts exhibited an ability to protect the DNA against devastating effects of Fentons reagent except methanol roots, stem bark, in vitro and callus extracts. The extracts have shown significant antioxidant activityand Phenol, flavonoid content was found to contribute towards antioxidant potential of C. fragrans....
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Posted by admin on Dec 1, 2014 in |
Direct Compression is becoming a preferred manufacturing process in order to speed up the manufacturing and reduce costs. Although a major requirement for direct compression process is selection of excipients with the optimum physical characteristics that increase final flowability and compressibility of the ready for compression (RFC) tableting blend. By using combinations of excipients and novel processing techniques, particles / excipients can be engineered to deliver desired properties to be used in direct compression. The resulting engineered excipients are commonly known as “coprocessed,” “high functionality,” “multifunctional,” “high functionality,” “performance” excipients. Prosolv Easytab SP and Panexcea™ MHC 300G are amongst this type of high-functionality excipients, which are used in present study to check their comparative performance on compression of tablets using direct compression technique with use of BCS class– II drugs such as Vardenafil HCl (5 mg). The drug was characterized using Infra-red spectroscopy. Pre-formulation, solubility and drug-excipient compatibility studies were carried for drug substance. While for all individual batches, blend analysis and various IPQC parameters such as tablet hardness,...
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Posted by admin on Dec 1, 2014 in |
Cancer plays crucial role in human life and critical disease to cure. Signaling pathways involved in stem cells development process. Especially, Wnt/β-catenin signaling pathway plays important role in stem cells self-renewal, differentiation and proliferation. Despite, over expression and mutation of Wnt/β-catenin signaling pathway induced variety of cancer development. Anticancer activities of several natural and synthetic agents were used to treat many cancer types. Many synthetic agents were used to cure various cancer but they have their toxicity. So, plant derived chemotherapeutic agents are going to investigate against cancer without side effect. In this paper we evaluate anticancer properties of Tradescantia spathacea medicinal plant and aberrant expression of β-catenin protein in human breast adenocarcinoma cell line (MCF-7). The plant leaves were collected, shade dried and extracted with hydroalcohol using soxhlet. Cytotoxic activities were assayed with MTT colorimetric procedure against MCF-7 cell lines. From the result, plant leaves extracts showed nearly 50 % MCF-7 cell line inhibition at 229.7µg/ml tested...
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Posted by admin on Dec 1, 2014 in |
A rapid, selective, precise, accurate, rugged and robust high performance liquid chrmatgraphic (HPLC) method for the simultaneous determination of Candesartan Cilexetil & Hydrochlorthiazide in tablet dosage form .The method was validated according to ICH and FDA guidelines. The chromatography is performed on a Kromasil C8, 150 x 4.6 mm, 5µm, in a gradient mode with a mobile phase of Water, acetonitrile and Trifluracetic acid in different ratios. UV-Visible detector at 285 nm was found to be suitable for detection. Linearity was observed in the range of 70 -130μg/ml with correlation coefficient of 0.9999. Sensitivity, accuracy, range, precision, robustness, ruggedness, stability, specificity, limit of detection, limit of quantification and system suitability parameters were validated for the developed method. The developed method was successfully applied to estimate the amounts in pharmaceutical formulations and for the stability indicating...
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Posted by admin on Dec 1, 2014 in |
In the present work various conventional methods and microwave method for synthesis of substituted quinolone reported. Substituted anilines were taken as a starting material and reacted with ethyl acetoacetate in presence of catalytic amount of acid and cyclized by various cyclisation agents that give the title compounds which differ in its color intensity, reaction time as well in %yield. Based on results we obtain, it has been concluded that microwave method for synthesis of substituted quinolone is more convenient in terms of reaction time and use of...
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