Posted by admin on Dec 1, 2014 in |
Cucurbitaceae commonly known as “Guard family” is distributed throughout India. Most of them, are perennial climbers with attractive flowers. The family includes plants having food as well as medicinal values. The species of the family are collectively known as cucurbits. They grow wild as well as cultivated throughout the state in Odisha in general and Similipal Biosphere Reserve (SBR) forest and its adjoining areas in particular. The major cucurbits available in SBR and its adjoining areas are Coccinia grandis L., Cucumis melo L., Lufa acutangula (L.) Roxb., Luffa cylindrica (L.) Roem., Solena amplexicaulis Lam., Mukia madraspatana (L.) Roem., Trichosanthus cucumerina L., Trichosanthus tricuspidata Lour.and Diplocyclos palmatus (L.) Jeffrey. The food values of some of these cucurbits have been assessed. Phytochemical analysis and antibacterial activity of the plant parts have shown potent medicinal values. Present paper highlights the diversity of wild cucurbits (WC) at SBR along with their nutritional and medicinal...
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Posted by admin on Dec 1, 2014 in |
Marine organisms are potentially prolific sources of highly bioactive secondary metabolites that might represent useful leads in the development of new pharmaceutical agents. The Libyan marine biodiversity including macroalgae remains partially unexplored in term of their potential bioactivities. Method: The phytochemical analysis of the alcoholic extracts of some commonly occurring seaweed Cystoseira compressa, Enteromorpha intestinals, Corallina and Ulva lactuca and their evaluated for antibacterial activity by well diffusion assay were studied. Four different solvents namely water, ethanol 99 %, methanol 99 %, and methylated spirit 95 % were used for extraction. Results: The phytochemical analysis revealed the presence of Carbohydrates, Steroids, Tannin & Phenols, Saponins, Proteins, and glycosides. The extracts were subjected for study of antibacterial activity. The zone of inhibition ranged between 8 to 16 mm in aqueous extract and up to 16 mm in methanol extract. The maximum activity (16 mm) was recorded from methanol extract of Ulva lactuca against Staphylococcus aureus and, minimum activity (8mm) recorded by Cystoseira compressa against Staphylococcus...
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Posted by admin on Dec 1, 2014 in |
Objectives: To evaluate the antidepressant activity of fresh leaves of Tinospora cordifolia in albino mice. Materials and Methods: Swiss albino mice weighing 25-30grams of either sex were selected for the study. Six animals were allocated to each experimental group. Animals were divided into 4 groups of 6 mice each. Group 1 – Control group treated with normal saline (0.1ml/10gm), Group 2 received standard group treated with 15mg/kg of imipramine, Group 3 – T1 treated with Tinospora cordifolia fresh leaves (100 mg/kg) and Group 4 – T2 treated with Tinospora cordifolia fresh leaves 50 mg/kg and 10 mg/kg of imipramine. Decrease in total immobility period denoted antidepressant activity in both tail suspension method & forced swim test in albino mice. Results: The test drug that is 50mg/kgp.o of Tinospora cordifolia fresh leaves along with 10mg/kg p.o of imipramine significantly decreased the total immobility period in both forced swim test and tail suspension method in albino mice. In forced swim test the percentage decrease in the immobility period was 38.68% when...
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Posted by admin on Dec 1, 2014 in |
Quorum sensing is a bacterial cell communication mechanism mediated through signal molecules such as AHL’s. Many opportunistic pathogens use these quorum sensing circuits to coordinate their virulence gene expression. Wide range of naturally occurring substances particularly plant extracts have been evaluated for their ability to modulate quorum sensing in Gram-negative bacteria. Preliminary screening of popular food spices for QSI activity revealed that Cinnamomum verum (Dalchini) had good QSI activity. Recognizing its medicinal value and dietary consumption, an attempt was made to evaluate QSI activity of Cinnamomum verum on bacterial pathogens like Pseudomonas and Serratia and confirmed by using bioindicator organism Chromobacterium violaceum. Cinnamomum verum extract had more QSI activity than the antibacterial activity in Serratia. In Pseudomonas, the antibacterial activity was more while QSI activity was comparatively less. Interestingly spice extract could reduce swarming nature in both the organisms, which is one of the important virulent factor regulated by QS. The phytochemical components in the crude extract were analyzed by qualitative methods. MS analysis of the treated samples revealed...
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Posted by admin on Dec 1, 2014 in |
A HPLC method has been described for simultaneous determination of Chlorpheniramine Maleate, Phenylpropanolamine Hydrochloride and Paracetamol in formulation. This method is based on HPLC separation of the three drugs on the Thermo Hypersil Gold C18 column(250mm ×4.6mm, 5.0µ), with isocratic conditions and mobile phase containing methanol: 0.01M disodium hydrogen phosphate dihydrate buffer pH 7 adjusted with Ortho Phosphoric Acid (OPA) (60: 40) at a flow rate of 1mL/min using UV detection at 217 nm. This method has been applied to formulation without interference of excipients of formulation. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 0.5-3µg/mL for Chlorpheniramine Maleate, 7-12µg/mL for Phenylpropanolamine hydrochloride, and 0.4-1.4µg/mL for Paracetamol, respectively. The mean values of the correlation coefficient, slope and intercept were 0.999 ± 1.72, 28455 ± 1.01, 26185 ± 1.28 for Chlorpheniramine Maleate, 0.999 ± 0.34, 23604 ± 1.16, 73758 ± 1.49 for Phenylpropanolamine hydrochloride and 0.999 ± 0.80, 51233 ± 1.89, 5560 ± 1.62 for Paracetamol respectively. The method...
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