Posted by admin on Jan 31, 2015 in |
This study was carried out with the objective of the antibacterial and antifungal activity of chloroform, ethyl acetate, ethanol, methanol and aqueous extracts of Asafoetida. Antibacterial activity was carried out against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and the antifungal activity was evaluated against Aspergillus niger, Candida albicans. The testing was done by well diffusion method and evaluation was done by detecting zone of inhibition (in mm) and minimum inhibitory concentration (MIC). Studies were perfomed with two different concentrations of all extracts (2 mg/ml and 4 mg/ml). Zone of inhibition were compared with standards like Ciprofloxacin (0.1 mg/ml) and Fluconazole (0.1 mg/ml). The results showed that ethyl acetate, ethanol, and methanol extract has significant antimicrobial activity and highest activity was reported with methanolic extract. The minimum inhibitory concentration of methanolic extract, ethanolic extract and ethyl acetate extract against most of the test microorganisms were 1 mg/ml, 1 mg/ml, 2 mg/ml...
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Posted by admin on Jan 31, 2015 in |
Two methods for simultaneous estimation of Pracetamol and Zaltoprofen in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 245 nm and 227 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 237.5 nm (isobestic point) and at 227 nm the maximum absorption of Zaltoprofen. The linearity ranges for Paracetamol and Zaltoprofen were 2 – 18 μg/ml and 2 – 18 μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 100.02 ± 0.467 and 99.87 ± 0.532 for simultaneous equation method and 99.82 ± 0.483 and 99.84 ± 0.512 for Q analysis (absorption ratio) method for Paracetamol and Zaltoprofen respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control...
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Posted by admin on Jan 31, 2015 in |
Methanolic extracts of leaf, bark, stem and root of D. paniculata were screened for phytochemicals and antibacterial activity against ten different pathogens. Various phytochemicals such as terpenoids, flavonoids, coumarins, saponins and tannins were found to be present. Leaf and bark extract exhibited maximum antibacterial activity against S. aureus, P. vulgaris and E. coli with MIC value of 2.5 mgmL-1. Significant antibacterial activity was shown by all extracts with MIC ranging from 10 mgmL-1 to 2.5 mgmL-1. Further isolation and characterization of phytochemicals could result in development of drugs against tested...
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Posted by admin on Jan 31, 2015 in |
Objective: To evaluate the pharmacognostic characters of Madhuca indica J.F. Gmel leaf and stem an important traditional medicinal plant. Method: In the present investigation, pharmacognostic parameters like macroscopic and microscopic and powder characters of leaf and stem were studied. Preliminary phytochemical and physiochemical analyses were done by using reported methods. Fluorescent behavior of the leaf and stem powder were also tested. Results: The macroscopic study showed that the leaf was simple and ovate with sinuate margin, acuminate at apex, decurrent at base and surface glabrous and venation was pinnate. The microscopic study of leaf revealed the presence of dorsiventral type of cellular arrangement, unicellular trichomes, prismatic crystals and anomocytic stomata; while the microscopic study of stem revealed the presence of vascular bundles in zig zag form, large central collenchymatous pith. Pith cells were polygonal in shape with minor angular thickenings. Physiochemical analysis of leaf showed total ash, water soluble ash and acid insoluble ash as 4.6, 0.83 and 0.5 %w/w respectively while in stem it was 5.37, 1.5 and...
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Posted by admin on Jan 31, 2015 in |
The compound 3-chloro-2-hydrazine quinoxaline was condensed with 2-furaldehyde to derive a Schiff base 2-furaldehyde-2-(3-chloro-2-quinoxalinyl) hydrazone (FCCQH).The ligand and also its complexes with VO (IV), Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Zn (II), and Pd (II) have been synthesized and characterized by elemental analysis, magnetic studies, thermal analysis, infrared spectra, UV-visible spectroscopy and Electron Spin Resonance data. The ligand 2-furaldehyde-2-(3-chloro-2-quinoxalinyl) hydrazone [FCCQH] acts as a neutral, bidentate one towards VO(IV), Zn(II), and Pd(II) coordinating through free C=N nitrogen and oxygen of furan ring and as tridentate one towards Cr(III), Mn(II), Fe(III), Co(II), and Ni(II) complexes coordinating additionally through nitrogen of quinoxaline ring C=N. The complexes have been screened for antibacterial activity towards staphylococcus aureus (gram +ve), Escherichia coli (gram -ve) bacteria, antifungal activity towards fusarium oxysporum and anticancer activities of the compounds were evaluated by MTT assay on HeLa (cervical cancer) cell lines. While the antimicrobial activity profiles of the complexes are, by and large quite encouraging, the anticancer activity exhibited by Ni (II) and Pd...
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