Posted by admin on Dec 1, 2014 in |
Alzheimer’s disease, the most common form of dementia accounting for about 50-60% of the overall cases among persons over 65 years of age is characterized by the progressive decline in cognitive function, mediated through learning and memory. According to Cholinergic hypothesis, AD is caused by reduced synthesis of the neurotransmitter ACh, wherein the AChE levels were increased which causes damage to the cholinergic neurons finally leading to cognitive impairments. Hence, all therapies for AD are targeted at the cholinergic system. In this context, docking studies play key role in computer-aided drug design paradigms. As an attempt to identify such natural cholinomimetic and neuroprotective activities, a set of 25 drug molecules from the phytoconstituents of the plant, Morinda citrifolia Linn. Were collected from PubChem Database. These compounds were docked against human AChE (PDB ID: 1B41 and 1N5R) proteins retrieved from Protein Data Bank were performed by Pyrex Virtual Screening tool (Auto Dock Vina). After docking, among these 25 compounds, the drug molecule Huperizine A (854026) was found to have the...
Read More
Posted by admin on Dec 1, 2014 in |
Rice is the most widely cultivated food crop over world, whose production is effected by many diseases like as fungal, bacterial and viral. Bacterial blight disease of rice caused by Xanthomonas oryzae. Chemical substance like Bordeaux mixture, copper and mercurial compounds have been used. Extensive use of these chemical causes harmful impacts on human and environment. For reducing problems associated to chemical substance, herbal plant like Aloe Vera gel and Cassia occidentals seed extract can be used for controlling disease cause through Xanthomonas oryzae (Xoo) in rice crop. The result of this study indicated that the Aloe Vera gel and seed extract of Cassia occidentalisplants contain some major bioactive compounds that inhibits the growth of the Xanthomonas oryzae and provides zone of inhibition, as Aloe Vera at 100% concentration provide diameter 14mm zone of inhibition in seed extract of Cassia occidentalis at 30mg/ml, 60mg/ml and 120mg/ml provide 5 mm, 9mm and 12mm diameter zone of inhibition respectively as It can be very important, for disease treatment in...
Read More
Posted by admin on Dec 1, 2014 in |
Herbal and herbo mineral preparations are being traditionally used in Indian system of Medicine especially in Siddha Medicine. Herbo mineral preparations have longer Shelf life. Rasa Chendooram (RC) is prepared as per classical text book of Prana Rakshamirtha Sindhu, for Vadha diseases. Before conducting clinical trial, Pre clinical study should be undergone as per WHO guidelines .The clinical trial has been approved by IEC (IEC NO-GSMC-CH/1/2013/011).The present preclinical study aims to carry out safety and toxicity of RC (IAEC NO.XXXXIX/07/CLBMCP/2013dated 29.06.2013) Adult both sex of Swiss albino rats weighing 220-250 gm were used. Acute & Sub acute toxicity were carried out as per OECD guidelines 423 and 407. Hematological Parameters, biochemical parameters histopathological study were performed for all animals. The study concludes that on oral administration of 100mg/kg of bodyweight of RC to Swiss albino rats, there was no change in behaviour movements and no characteristic clinical sign of toxicity or mortality...
Read More
Posted by admin on Dec 1, 2014 in |
The combination of Nigella sativa seed oil and ginger extract is used as antiulcer agent and to treat abdominal pain, diarrhoea and flatulence. The paper describes validated simultaneous equation method for the simultaneous estimation of Nigella sativa seed oil and Ginger extract in a combined dosage form. Nigella sativa seed oil and Ginger extract were found to have absorption maxima at 256 and 282 nm respectively in 5% SLS solution. Both these drugs obeyed Beer’s law in the concentration range of 100-500μg/mL. The high values of correlation coefficients (r2) indicated good linearity of calibration curve for both the drugs. The results of analysis have been validated statistically and by recovery study the value of standard deviation ranging from 97-99.21 % for Nigella sativa seed oil and 98-101.6 % for ginger extract were indicative of the accuracy and precision of the proposed method. The proposed method was successfully applied for the determination of nigella sativa seed oil and Ginger extract in the pharmaceutical formulation. This method was found to be...
Read More
Posted by admin on Dec 1, 2014 in |
Chonemorpha fragrans (Moon) Alston is a liana belongs to family Apocynaceae. Due to its ethanobotanical importance, it is used intraditional medicinal systems as a part of various medicinal preparations. In the work presented here the plant has been explored for its antioxidant potential and DNA protection ability. The antioxidant potential of C. fragrans was determined using DPPH, FRAP, Phosphomolybdanu and H2O2 Scavenging Capacity. Phenol and flavonoid content was measured followed byCorrelation analysis between antioxidant activities andphenols and flavonoid content. In addition, DNA protection ability of extracts was evaluated using double stranded plasmid DNA. C. fragrans extracts have shown significant antioxidant potential. Phenol content showed high correlation with Phosphomolybdenum reduction potential where as flavonoid content with Phosphomolybdenum as well as H2O2 radical scavenging potential. All the extracts exhibited an ability to protect the DNA against devastating effects of Fentons reagent except methanol roots, stem bark, in vitro and callus extracts. The extracts have shown significant antioxidant activityand Phenol, flavonoid content was found to contribute towards antioxidant potential of C. fragrans....
Read More
