Posted by admin on Dec 25, 2014 in |
Madhucalongifolia is an economically important medicinal tree growing throughout the subtropical region. This research article deals with the antioxidant and antimicrobial properties of M. longifolia. The antioxidant properties of the seed were determined by hydrogen peroxide scavenging activity and reducing power assay. The methanol extract exhibited more antioxidant activity than water extract. The fungal and bacterial pathogens used for the study were isolated from the skin scraping of infected animals. The methanol and water extracts of M. longifolia seedswere tested for their antimicrobial efficacy against the pathogens. Methanol extract exhibited good antimicrobial property than water extract. From the above findings it is confirmed that the seeds of M. longifolia posses good antioxidant and antimicrobial...
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Posted by admin on Dec 25, 2014 in |
Objectives: The study aimed to isolate coumarin compound from Bauhinia purpurea bark. Method: The bark was extracted successively with methanol in a soxhlet apparatus. The crude mass was subjected to HPLC, as well as column chromatography and eluted with methylene dichloride – methanol system with increasing methanol ratio to isolate compound BP. The structure of compound BP was identified by various spectrophotometric techniques (UV, IR, 1HNMR, 13CNMR and Mass Spectrometry). Key Findings: Compound BP was obtained as a white crystalline solid with melting point 197oC. It responded positively to the test of flavonoids. It showed λmax at 256 and 356 nm, IR bands at 3258.93, 1343.97, 1655.57, 1165, 1198.52 cm-1, NMR spectra confirmed the presence of phenolic OH and coumarin ring and [ESI-MS] m/z 462.9 [M+– 1]. Conclusion: Concluding from the interpretations of all the spectra, the compound BP was identified as 3, 5, 7- trihydroxy – 2 – (3, 4, 5- trihydroxy phenyl) – 4H-1 – benzopyran – 4- one. This compound had been found worthy for pharmacological...
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Posted by admin on Dec 25, 2014 in |
Tannins, the non-nutritive substances from plant sources exhibit potent biological activities that lower the risk of chronic diseases without any side effects. Tannins are most abundant and multipotent molecules exhibiting antioxidant, proapoptotic and antiangiogenic activity and thus being effective in the treatment of degenerative diseases including initiation and progression of tumors. The study aims to prove the chemopreventive properties of tannins that target several key events involved in the development of cancer. Partially purified tannins from the acetone extract of Memecylon malabaricum was subjected to shell less CAM assay and in vitro studies to note the antiangiogenic and antioxidant activities respectively, while the proapoptotic activity was noted by its effect on EAT cells. Inhibiting the proliferation of capillaries in shell less CAM assay proved their antiangiogenic property. 1, 1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity (EC50 of 2.67 µg/ml), Hydroxyl radical scavenging activity (EC50 of 7.73 µg/ml), Nitric oxide radical scavenging activity (EC50 of 11.3 µg/ml) and superoxide anion radical scavenging activity (EC50 of 19 µg/ml) established their antioxidant activity,...
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Posted by admin on Dec 25, 2014 in |
The two pure phytochemicals characterized as cis-piperine and trans-piperine isolated from the dried fruits of Piper longum were tested for antihepatotoxic activity at the dose of 50 mg/kg orally respectively, against silymarin as a standard reference. The toxicity was induced by CCl4, and then various biological parameters such as AST, ALT, ALP, SOD, GPx, GSH, total protein, total albumin and total bilirubin were measured at 134.24 IU/L 71.96 IU/L, 375.02 IU/L, 37.02 U/g, 17.02 U/g, 29.12 U/g, 1.95 g/dl, 1.09 g/dl, 4.16 g/dl respectively. The phytochemicals cis-piperine and trans-piperine showed significant antihepatotoxic activity. Both cis-piperine and trans-piperine exhibited antihepatotoxic activity by reducing the increased levels of serum AST by 90.14%, 91.19% ALT by 82.65%, 58.12% and ALP by 97.37%, 92.23% when compared with standard drug silymarin that have decreased AST by 91.64%, ALT by 89.92%, ALP by 94.54%, respectively, and by elevation in the antioxidants SOD, GPx, and GSH) respectively, and total albumin, total protein. The overall experimental results have suggested that the both the isomers of piperine possessed...
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Posted by admin on Dec 25, 2014 in |
The aim of this study was to investigate the possible anticonvulsant effect of a Dihydropyridine calcium antagonist, Isradipine, which easily crosses the blood–brain barrier displaying high affinity and specificity for the brain L-type voltage-sensitive calcium channel, on maximal electroshock seizures and PTZ induced seizures. The seizures were induced in rats by maximum electroshock method and chemically by pentylenetetrazole (PTZ). Isradipine delayed the onset of action and time of death to 213.21 ± 32.46 seconds and 952.85 ± 9.81 seconds respectively when compared with control in PTZ induces seizures model. The present study indicates that Isradipine showed significant anticonvulsant effect against maximal electroshock and PTZ induced convulsion in...
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