Posted by admin on Nov 1, 2014 in |
Present investigation deals with the evaluation of the antimicrobial activity of extracts of Jatropha gossypifolia Linn., Sapium sebiferum (L.) Roxb., Kirganelia reticulata (Poir) Baill., Phyllanthus fraternus Webster and Pedilanthus tithymaloides (Linn.) Poit. against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus and Trichophyton mentagrophytes and T. rubrum. The antibacterial activity was tested by the disc diffusion method and the antifungal (antidermatophytic) activity by the method of Abubacker et al. It was observed that only DMSO extracts of the leaves of K. reticulata and the flowers of P. tithymaloides inhibited the growth of P. vulgaris and S. aureus with MIC at 200 mg ml–1. The extracts did not inhibit the growth of T. mentagrophytes and T. rubrum. The phytochemical analysis of these extracts indicated that the tannin and saponin contents were highest in the leaves of K. reticulata (6.0 and 12.04 % respectively) whereas, the flavonoid and steroid and triterpenoid contents were highest in theflowers of P. tithymaloides (41.20 % and 46.74 %). The MIC values of extracted tannins...
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Posted by admin on Nov 1, 2014 in |
Diallyl Disulphide is an essential component of garlic which has numerous medicinal applications. Thus it was evaluated for its free radical scavenging potential. The compound was assayed for its ability to stabilize the erythrocyte membrane by osmotic fragility test and heat induced hemolysis assay. The thrombolytic potential was evaluated by the ability of the compound to dissolve the clot formed. The antioxidant studies were assessed by DPPH radical scavenging assay, ferric reducing antioxidant potential, Superoxide radical scavenging potential and Nitric oxide radical scavenging potential. The total antioxidant capacity was also estimated and the values were expressed in terms of Ascorbic acid equivalents. The results show a poor free radical scavenging potential and antioxidant capacity. However it has shown a moderate percentage in dissolution of clot implicating in thrombolytic potential. The compound has also shown a higher erythrocyte membrane stabilization by reducing the percentage of hemolysis in heat induced hemolytic assay than Ascorbic Acid. The results suggest that Diallyl Disulphide is a poor antioxidant but a potential anti-hemolytic in In-vitro...
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Posted by admin on Nov 1, 2014 in |
Two simple, sensitive and rapid spectrophotometric methods was developed for estimation of glibenclamide in pure and in pharmaceutical dosage forms. Method-A is a uv spectroscopic method with the absorption maxima at 276nm.Method-B is based on the oxidation/reduction of glibenclamide with iron (III) and chelation of iron with 3-methyl 2-benzothiozoline hydrazone(MBTH) to form green colored complex with absorption maxima at 630nm.Calibration curve was plotted. The assay was validated for the parameters like precision, accuracy, robustness, ruggedness, LOD and LOQ. The proposed methods can be useful for the routine analysis for the determination of glibenclamide in pharmaceutical dosage...
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Posted by admin on Nov 1, 2014 in |
A rapid and sensitive pH-gradient SAX HPLC method for the determination of Dermatan sulphate (DS) and trace levels of Oversulphated chondroitin sulphate (OSCS) in Heparin sodium was developed and validated. A pH gradient was opted to strike a balance between resolution and sensitivity because the response of OSCS was found to be better at neutral pH and the resolution between DS and Heparin was good at acidic pH. The use of commercially available lithium perchlorate was avoided and an in situ preparation of the same was opted to decrease the baseline drift which is a major concern for gradient methods using perchlorate mobile phases for compounds being analyzed at lower wavelengths. Chromatographic separation was achieved using a hydrophilic polymer resin-based SAX column, a linear gradientelution from 15% to 70% of solution-B which is 1.0M lithium perchlorate containing 5mM sodium dihydrogen phosphate at pH 6.0 and solution-A being 5mM sodium dihydrogen phosphate at pH 3.0 and a UV detection at 202 nm. The method has been validated and the limit...
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Posted by admin on Nov 1, 2014 in |
The aim of the study is to screen the grasses of South India for mineral nutrition. The results revealed that the grass species accumulate the major and minor elements at different concentrations. The maximum concentration (ppm) of macroelements in the analysed samples were 12270, 31310, 5309 and 3946 for Ca, K, Mg and P respectively. The maximum concentrations (ppm) of microelements were 50.05, 89.68, 2452, 258.3, 64.01 and 76.05 for B, Cu, Fe, Mn, Mo and Zn respectively. 22% of ash content and 4192 cal/g of calorific value were found. The levels of macro and micro elements determined of the grasses were analysed by using ICP –OES. Elemental studies of the grass species revealed that large amounts of major and minor elements were rich in Aristida hystrix and Echinochloa colonum, which may be suggested for livestock...
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