Posted by admin on Sep 1, 2014 in |
The present study was aimed to investigate the methanolic (MEMP), ethyl acetate (EEMP) and n-hexane (HEMP) extracts of the whole plant of Mucuna pruriens for their in-vitro Antiurolithiatic potential. Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine. The in-vitro anti-urolithiatic activity of various extracts of M. pruriens was investigated by measuring the turbidity of various concentrations of (50µg m/ml, 100µg m/ml, 150µg m/ml, 200µg m/ml, and 250µg m/ml) each extract in synthetic urine, by using UV/Visible spectrophotometer at 620 nm after incubating them for 10 min and microscopically under microscope equipped with digital camera at 10X. The results of the present study showed that the MEMP, EEMP dose-dependently inhibited the formation of calcium oxalate (CaOx) crystallization in the synthetic urine which was evident from the spectroscopic as well as in the microscopical studies. It was concluded that the antiurolithiatic activity of Mucuna pruriens was possibly mediated through inhibition CaOX crystals in the synthetic urine. But, further, in-vivo, preclinical studies are required...
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Posted by admin on Sep 1, 2014 in |
Objective: To study the nephroprotective activity of Bauhinia purpurea unripe pods and bark against gentamicin-induced nephrotoxicity. Materials and Methods: Twenty four healthy adult albino rats of either sex (150-200 g) were randomly divided into four groups of six animals each. Group, I served as vehicle control and Group II as the negative control (gentamicin 80 mg/kg/d, i.p). Groups III and IV (test groups) were administered ethanolic extract of Bauhinia purpurea bark (BBE) and unripe pods (BPE) in the dose of 400 mg/kg/day, one hour before gentamicin administration. All the treatments were given for 8 days. On the 9th day, blood and urine samples were used for biochemical analysis and kidneys for histology and assessing anti-oxidant activity. Results: Gentamicin caused nephrotoxicity as evidenced by highly significant (p<0.001) elevation of blood urea, serum creatinine and urine glucose in Group II as compared to Group I. Also administration of Gentamicin in group II caused oxidative stress in rats suggested by significant increase in MDA level and significant decrease in Catalase and Reduced...
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Posted by admin on Sep 1, 2014 in |
The kinetics of oxidation of quinine sulphate [8α, 9R-61– methoxycinchonan- 9 – ol sulphate], (QS) by Chloramine-B (Sodium N-Chlorobenzene Sulphonamide, CAB) has been studied in aqueous hydrochloric acid medium at 303 K. The reaction rate shows a first order with respect to [CAB], fractional order with respect to [HCl] and [QS]. Activation parameters were evaluated from the kinetic data at different temperatures. Negative entropy of activation indicated the involvement of a rigid complex in the activated state. The dielectric constant of the medium has a small effect on the rate. Ionic strength and the reduction product benzene sulphonamide [BSA] do not affect the reaction rate. The solvent isotope effect is studied. The reaction products are identified and characterized by spectral (IR and NMR) data. The proposed reaction mechanism and the derived rate equation are consistent with the observed kinetic...
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Posted by admin on Sep 1, 2014 in |
Overproduction of free radicals can cause oxidative damage to biomolecules (e.g., lipids, proteins, DNA), eventually leading to many chronic diseases, such as atherosclerosis, cancer, diabetes, aging, and other degenerative diseases in humans. Plants may contain a wide variety of free radical scavenging molecules, such as phenolic compounds, nitrogen compounds, vitamins, terpenoids, and some other endogenous metabolites, which are rich in antioxidant activity. In the present study in-vitro antioxidant potential of various fractions of Hibiscus rosa sinensis petal (HRS) ethanolic extract was investigated. Fractions prepared from crude ethanolic extract of HRS were concentrated and used for various in-vitro assays. Ethyl acetate fraction of HRS (EHRS) had a very high content of total phenolics and flavonoids. DPPH radical scavenging activity (IC50-9µg/ml) of EHRS was comparable with standard Quercetin. Superoxide, ABTS, and hydroxyl radical scavenging activities of EHRS were also found higher than other fractions. The total antioxidant activity and reducing power were also high in EHRS. So the study suggests that EHRS may be a potential source of bioactive substances with...
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Posted by admin on Sep 1, 2014 in |
ABSTRACT: Celastrus paniculatus is a known herb used in alternative system of medicines since ancient times. Traditionally seed oil of Celastrus paniculatus has been in various medicinal remedies to cure neuronal disorders, asthma, cough, etc. The constituents found in Celastrus paniculatus reportedly are sesquiterpenoids, triglycerides, triterpenoids. A high-performance thin layer chromatography, which is considered as a sensitive method for accurate quantification method for analysis of compounds. The instrument CAMAG Linomat 5 lamp was used, and the wavelength was 254...
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