Posted by admin on Dec 1, 2014 in |
Background: Drug resistance among Candida species constitutes the most significant problem in the treatment of Candidiasis.Systematic studies on antifungal drug susceptibility which may be useful in deciding clinical strategies are not routinely done in India and other developing countries. Objective: Aim of this study was testing sensitivity of clinical isolates of Candida albicans and non albicans to frequently prescribe antifungal drugs, fluconazole, ketoconazole, Itraconazole, Amphotericin B and Terbinafine. Material and methods: 25 strains of C. albicans and non albicans were tested in vitro for susceptibility to five antifungal agents, by using standard broth macro dilution method (CLSI M 27-A). Results: The present study revealed the percentage and extent of emerging drug resistance and cross resistance among Central Indian clinical isolates of C. albicans and non albicans against azoles and allylamine. In the present study, the total percentage of resistance was found to be 84% (21/25). The drug for which maximum resistance were found was Ketoconazole (64%) followed by Itraconazole (44%) Terbinafine (24%) and lastly Fluconazole (20%). The total percentage...
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Posted by admin on Dec 1, 2014 in |
Some new Sb(III) and As(III) complexes of N∩S donor ligands N’-[1-(2-oxo-2H-chrome-3yl-ethylidene] – hydrazinecarbodithionic acid methyl ester (L1H) and N’ – [1-(2-oxo-2H-chrome-3yl – ethylidene] -hydrazinecarbodithionic acid benzyl ester (L2H) have been synthesized. All the synthesized compounds were characterized by elemental analyses, melting point determinations and a combination of electronic, IR, 1H NMR, and X-ray diffraction spectroscopic techniques. These studies showed that the ligands coordinated to organoantimony and organoarsenic in a monobasic bidentate manner through sulfur and nitrogen donor system. Thus, tetra and pentacoordinated environments around the antimony and arsenic atom have been proposed. In order to evaluate the effect of metal ions upon chelation, both the ligands and their complexes have been screened for their antimicrobial activity against the various pathogenic bacterial and fungal strains. The results indicate that the metal complexes are more potent antimicrobial agents than the parent ligands. Further, the ligands and their corresponding metal complexes have been tested for their pesticidal activity against the Corcyra cephalonica. The results of pesticidal screening are quite...
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Posted by admin on Dec 1, 2014 in |
A simple, sensitive, linear, precise and accurate RP-HPLC method for simultaneous estimation of Zaltoprofen and Paracetamol in bulk and tablet formulation was developed and validated. The proposed RP-HPLC method utilizes Phenomenex C18 column (150 ×4.6 mm i.d., 5 µm), optimum mobile phase consisted of isocratic run of Phosphate buffer (pH-4.2): acetonitrile (40:60V/V) with the effluent flow rate of 1.0ml/min, and UV detection wavelength 260 nm. The developed method was statistically validated for linearity, precision, robustness, ruggedness and specificity. The method was linear over the range of 2-10 µg/ml for Zaltoprofen and 8-40 µg/ml for Paracetamol. The mean recovery was 99.27% and 99.76% for Zaltoprofen and Paracetamol respectively. The intermediate precision data obtained under different experimental setup was quite concurrent with less critical %RSD. The proposed method can be useful in the quality control of Zaltoprofen and Paracetamol in their combined dosage...
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Posted by admin on Dec 1, 2014 in |
Aims: Detection and isolation of restriction endonucleases like topoisomerase Thermophilic Bacillus subtilus strain RC1. Methods and results: Topoisomerase like enzyme activity is detected from thermophilc Bacilus sp. The isolated enzyme is partially characterized. The molecular weight of the enzyme is ~ 33 KDa and the optimum temperature for the activity is 37 0C. The crude and gel purified enzyme activity were performed. The enzyme could be novel one, as the isolate is from hot water ecosystem. The restriction pattern of the new isolated enzyme is dissimilar to the commercially available enzymes. The enzyme retained the activity at a storage temperature of 4 0C about one to two months. Magnesium chloride is found to be essential for the activity. The enzyme activity pattern is like topoisomrase. Activity is enhance in presence of ATP. The further characterization of topo like enzyme is in contined.Conclusion: The endonuclease type of topoisomerase enzyme is detected and isolated from thermophilic Bacillus sp.The restriction pattern studies indicate that the enzyme cuts standard plasmid DNA. Significant impact...
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Posted by admin on Dec 1, 2014 in |
The present chemical analysis deals with Asparagus racemosus root for phytochemical screening and GC-MS analysis. Phytochemical constituent of the root extract was carried out according to the standard method. GC-MS analysis was performed on the methanolic extract of A. racemosus to find out the chemical constituents. The phytochemical screening revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, terpenoids, saponins. GC-MS results revealed the presence of 6 different phytochemical constituents like, 2-Propanone, 1,3-dihydroxy 1), 2-Fruancarboxy aldehyde, 5-(hydroxymethyl) 2), Hexadecanoic acid 3), n-Hexadecanoic acid 4), Ethanol,2(Octyloxy)- 5), 1,9-Nonanediol 6). The presence of various biological active compounds confirms the application of A. racemosus for various diseases by conventional practitioners. The obtained phytochemical constituent results demonstrate that the methanol root extract of Asparagus racemosus could be used corn foot, anticancer, diabetic healer. However, isolation of individual phytochemical constituents might be useful to formulate a novel...
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