Posted by admin on Dec 1, 2014 in |
A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for the determination of Lamivudine, Tenofovir and Efavirenz simultaneously in combined tablet dosage form was developed. Chromatography was performed with mobile phase containing a mixture of methanol: Water (pH adjusted to 2.5) and 0.1 % TEA in the proportion of (68: 32 % v/v) the samples were injected onto Symmetry C18 Column (4.6 x 100mm, 5μm, Make: HYPERSIL ODS) column. The flow rate was 1.2ml.min–1. The samples were detected at 260nm. The UV spectrophotometric method was performed at 272nm for Lamivudine, 260 nm for Tenofovir and 247 nm for Efavirenz, and samples were prepared with a solution of Water methanol (30:70 % v/v). The assay was linear in range from 25% to 150% targeted concentration and regression coefficient for all three drugs was found to be 0.999 highly significant for the method. The proposed methods were simple, rapid, precise, accurate and sensitive, and can be used for the routine of the quality control in...
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Posted by admin on Dec 1, 2014 in |
Adhatoda vasica Nees. has been used in India for more than 2000 years. The drug contains leaf, stem, flower, fruit and seeds. The fruit which holds the most potentialof the herb is a small capsule with four seeds. No reports are available for the pharmacognostical study of the seed, hence the present study was undertaken to investigate the macroscopic, microscopic, powder microscopic, physicochemical, phytochemical analysis, TLC and HPTLC profile. The drug was mounted on FAA solution and sections were taken in rotary microtome, stained with toluidine blue; histochemical tests were observed. Loss on drying, total ash, water soluble and acid insoluble ash, water and alcohol soluble extractive were estimated as per WHO method. TLC/HPTLC studies were based on many trials to fix the better solvent system. The testa comprises outer sclerotesta of 40mm thick and inner sarcotesta. The parenchymatous zone is thin along the lateral part of the seed and it becomes wider and many layered at the chalazal end. The radicle is circular in sectional view which is...
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Posted by admin on Dec 1, 2014 in |
Alzheimer’s disease, the most common form of dementia accounting for about 50-60% of the overall cases among persons over 65 years of age is characterized by the progressive decline in cognitive function, mediated through learning and memory. According to Cholinergic hypothesis, AD is caused by reduced synthesis of the neurotransmitter ACh, wherein the AChE levels were increased which causes damage to the cholinergic neurons finally leading to cognitive impairments. Hence, all therapies for AD are targeted at the cholinergic system. In this context, docking studies play key role in computer-aided drug design paradigms. As an attempt to identify such natural cholinomimetic and neuroprotective activities, a set of 25 drug molecules from the phytoconstituents of the plant, Morinda citrifolia Linn. Were collected from PubChem Database. These compounds were docked against human AChE (PDB ID: 1B41 and 1N5R) proteins retrieved from Protein Data Bank were performed by Pyrex Virtual Screening tool (Auto Dock Vina). After docking, among these 25 compounds, the drug molecule Huperizine A (854026) was found to have the...
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Posted by admin on Dec 1, 2014 in |
Rice is the most widely cultivated food crop over world, whose production is effected by many diseases like as fungal, bacterial and viral. Bacterial blight disease of rice caused by Xanthomonas oryzae. Chemical substance like Bordeaux mixture, copper and mercurial compounds have been used. Extensive use of these chemical causes harmful impacts on human and environment. For reducing problems associated to chemical substance, herbal plant like Aloe Vera gel and Cassia occidentals seed extract can be used for controlling disease cause through Xanthomonas oryzae (Xoo) in rice crop. The result of this study indicated that the Aloe Vera gel and seed extract of Cassia occidentalisplants contain some major bioactive compounds that inhibits the growth of the Xanthomonas oryzae and provides zone of inhibition, as Aloe Vera at 100% concentration provide diameter 14mm zone of inhibition in seed extract of Cassia occidentalis at 30mg/ml, 60mg/ml and 120mg/ml provide 5 mm, 9mm and 12mm diameter zone of inhibition respectively as It can be very important, for disease treatment in...
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Posted by admin on Dec 1, 2014 in |
Herbal and herbo mineral preparations are being traditionally used in Indian system of Medicine especially in Siddha Medicine. Herbo mineral preparations have longer Shelf life. Rasa Chendooram (RC) is prepared as per classical text book of Prana Rakshamirtha Sindhu, for Vadha diseases. Before conducting clinical trial, Pre clinical study should be undergone as per WHO guidelines .The clinical trial has been approved by IEC (IEC NO-GSMC-CH/1/2013/011).The present preclinical study aims to carry out safety and toxicity of RC (IAEC NO.XXXXIX/07/CLBMCP/2013dated 29.06.2013) Adult both sex of Swiss albino rats weighing 220-250 gm were used. Acute & Sub acute toxicity were carried out as per OECD guidelines 423 and 407. Hematological Parameters, biochemical parameters histopathological study were performed for all animals. The study concludes that on oral administration of 100mg/kg of bodyweight of RC to Swiss albino rats, there was no change in behaviour movements and no characteristic clinical sign of toxicity or mortality...
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