Posted by admin on Aug 1, 2014 in |
The aim of the present study was to determine the antibacterial activity of crude extracts from root parts of Catharanthus roseus against several bacterial species of clinical significance. Root part of C. roseus was extracted in appropriate solvent followed by evaluation of antibacterial activity by agar well diffusion assay against a total of eight bacterial stains. Further, minimum inhibitory concentration(s) was evaluated for the crude extracts. Among all the extracts, the chloroform extract was found to be most active against almost all the bacterial species tested. Gram-positive bacteria were found more sensitive than Gram-negative bacteria. Other focuses included the determination of antioxidant activity using DPPH assay and IC50 (Inhibitory concentration) values were also determined using broth dilution assay. Preliminary phytochemical screening of the crude chloroform extract revealed the presence of alkaloids, flavonoids, tannins, saponins, proteins and phenolics. The study promises an interesting future for designing potentially active antibacterial agents from Catharanthus...
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Posted by admin on Aug 1, 2014 in |
Achyranthes aspera (Amaranthaceae) is an important medicinal herb found as a weed throughout India. It has been used in almost all the traditional system of medicine, ayurveda, unani, and sidha from the ancient time. It serves as a folk medicine in traditional uses. In present study, the methanolic extract and aqueous extracts of leaf, stem, inflorescence and roots of Achyranthes aspera was screened. The Physio-Chemical Parameters of Achyranthes aspera like determination of moisture content, total ash content, acid insoluble ash content, water soluble ash content and solvent extractive values were done. Qualitative phytochemicals revealed the presence of alkaloids, saponins, flavonoids and glycosides in each extract. In A.aspera extract the moisture content, total ash content, insoluble ash content in stem and roots was more than leaf and inflorescence and water soluble ash content was found highest in inflorescence followed by stem then leaf and least in...
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Posted by admin on Aug 1, 2014 in |
A new, precise, rapid, accurate RP-HPLC method was developed for the estimation of Decitabinein pharmaceutical dosage form. After optimization the good chromatographic separation was achieved by Isocratic mode with a mixture of Ammonium Acetate buffer of pH 4.5: Acetonitrile (985:15) v/v as the mobile phase withDevelosil Rp Aqueous-AR-5 (150 x 4.6 mm, 5 µm), column as stationary phase at flow rate of 1.5 mL/min and detection wavelength of 244 nm. The Retention time of decitabine was found to be 3.796 min. The linearity of this method was found in the concentration range of 50-150 µg/mL. The correlation coefficient R2value is found to be0.998. The LOD for this method was found to be 0.0003 µg/mL. The LOQ for this method was found to be 0.0009 µg/mL. This method was found to be good percentage recovery about 99.77 indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the...
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Posted by admin on Aug 1, 2014 in |
An accurate, precise and reproducible UV-spectro-photometric methods and liquid chromatographic assay method were developed and validated for the determination of Levofloxacin and Ornidazole in tablet dosage form. Spectrophotometric estimation was done by simultaneous equation method and 50% methanol as solvent. In this method λmax for LEVO and OZ were selected at 293.5nm and 318nm. RP-HPLC analysis was carried out using Prontosil C-18 column (4.6 x 250mm, 5μ particle size) and mobile phase composed of Acetonitrile : 0.05% Ortho-phosphoric acid in water pH 3.0 (45:55% v/v)at a flow rate of 1.0 ml/min and chromatogram was recorded at 303 nm. Linearity was evaluated over the concentration range of 4 -20 μg/ml and 8-40μg/mL for LEVO and OZ in both UV spectrophotometric and RP-HPLC method (the value of r2= 0.999 found were by both the methods for LEVO and OZ). The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both...
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Posted by admin on Aug 1, 2014 in |
The aim of this study was to extract the secondary metabolites of Coscinium fenestratum stem extracts and to determine the alkaloid berberine using HPLC. Phytochemical analysis was carried for acetone, chloroform, methanol and Aqueous fractions of stem extracts. Total phenolic content and total flavonoid contents were estimated using standard Gallic acid and Quercitin respectively. TLC and HPLC were performed for alkaloid to determine the berberine content present in the stem extract. Preliminary phytochemical screening revealed the presence of alkaloids, saponins, steroids, phenolic substances and cardiac glycosides. Steroids and tannins were absent in the stem extract. The stem methanolic extract of C.fenestratum contained large amount of phenolics18.35±0.56mg GAE/g extract and the flavonoid was found to be 12.8±0.88 mg QE/g extract respectively. The concentration of berberine in the stem was found to be...
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