Posted by admin on Oct 1, 2014 in |
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantization of Ceftriaxone (CEF) and Sulbactam (SUL) in parenteral preparation. Chromatographic separation was carried out in a Hypersil Gold C8 column (250 mm × 4.6 mm; 5 μm particle size) of Thermo Scientific with simple mobile phase composition of 10 ml of 40% Tetra Butyl Ammonium Hydroxide(TBAH) in 1000 ml of water (pH 5.5, maintained by dil Phosphoric acid) and acetonitrile (70:30, v/v) at a flow rate of 2.0 ml min-1 with injection Volume of 20 µl where detector was set at 227 nm with a total run time of 10 min. The method was linear over the concentration range of 40-100, μg ml-1 for SUL and 80-200 μg ml-1 with a correlation coefficient of 0.999 and 0.999 respectively. Limit of quantifications (LOQ) of 8.5, 14.4 and limit of detections (LOD) 2.8, 4.7μg ml-1 for CEF and SUL respectively. Accuracy and precision values of both...
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Posted by admin on Oct 1, 2014 in |
A simple, sensitive, cost-effective spectrophotometric method has been developed and validated for the determination of Ambroxol hydrochloride (AMB), in bulk drug and its pharmaceutical formulations. The method is based on the formation of a Schiff base with PDAB; the reaction of drug with reagent gives a bright yellow color. The so formed colored species absorbance was measured at its absorption maximum (λmax) 423 nm. Beer’s law has been obeyed in the concentration range 5-30 µg/ml. The optical parameters were calculated as 2.0702 × 104 (L/mol/cm), 0.0183 (µg/cm2), molar absorptivity and Sandell sensitivity respectively. The LOD and LOQ of the proposed method were calculated 0.0844 (μg/ml), 0.2812 (μg/ml), respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing AMB in its pharmaceutical formulations and critically tested for its accuracy by statistical tests. Good recoveries were obtained by the developed method; the obtained results were critically analyzed and successfully employed...
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Posted by admin on Oct 1, 2014 in |
Lepidium sativum Linn. locally known as ‘fetto’ is a fast-growing edible herb which belongs to the family Brassicaceae is traditionally used for the treatment of various human ailments including cold, headache, colic, abdominal pain, dysentery, swellings and aphrodisiac in Ethiopia. Phytochemical screening and antimicrobial activities were conducted on seeds of Lepidium sativium grown in Ethiopia. Qualitative phytochemical screening test of chloroform/ methanol crude extract showed the presence of phytochemicals: flavonoid, cholesterol, terpenoids, steroids, carbohydrates, glycosides, tannins, alkaloids, phenols, phytosterols, proteins, and saponins. Antimicrobial activities of crude extract were tested against four bacteria: two Gram-negative bacteria (Escherichia coli and Salmonella typhi) and two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtillis) and three fungi (Aspergillus niger, Fusarium oxysporum, and Fusarium solani). The crude extracts of the seeds of the plant were active against the tested bacteria and fungi. The antimicrobial activities of plant seeds crude extract were compared with that of chloramphenicol against bacteria and bavistin against fungi as reference antibiotics. The ethnopharmacological knowledge of Lepidium sativum was documented. It was...
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Posted by admin on Oct 1, 2014 in |
The prevalence of psoriasis has a deep negative impact on Quality of life (QoL) daily. It has been classified under psychophysiological disorders. The prime objective of the present study is to evaluate and assess the Quality of Life (QoL) of psoriasis patients in Osmanabad region of India. A retrospective analyzing study was carried out on 52 psoriatic patients attending outpatient and inpatient departments of the Government Ayurvedic College and Hospital, Osmanabad, Maharashtra state, India. The study was carried out between October 2010 to March 2011. A total of 52 patients suffering from psoriasis who had completed an assessment of disease severity and the dermatology life quality index (DLQI) questionnaire were analyzed. Of these patients, 75% were men. The mean score on the DLQI was 8.28, and almost all patients reported a moderate to extremely large impact on their quality of life (DLQI >7). A higher psoriasis area severity index (PASI), younger age, and initial lesions on the trunk, hands-legs, and scalp significantly negatively impacted patients quality of life. Psoriasis...
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Posted by admin on Oct 1, 2014 in |
To evaluate the effects of different processing parameters on drug-loaded microspheres, the solvent evaporation technique was chosen as the formulation method. Indomethacin-loaded Polylactic acid microspheres were prepared by varying drug masses, then by changing the stirring speed. They were analyzed for their drug content, physical morphology, size, polymer-drug physicochemical compatibility, and in-vitro drug release. Microspheres revealed a spherical profile along with a porous structure. The effect of changing the drug mass was noted on drug encapsulation and loading efficiencies. Indomethacin had better encapsulation when introduced in small amounts. On the other hand, the effect of changing the stirring speed was remarkable on the size of obtained microspheres and drug release profile. The particle size decreased as the speed of mixing increased. Also, a faster drug release was observed for larger...
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